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Citations for Merck :
3151 - 3200 of 3401 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 2 h at room temperature with the following affinity pure primary antibodies: rabbit polyclonal anti-TRPM4 (Cat.# ABN418; Merck Millipore, Darmstadt, Germany) and mouse monoclonal anti-Aquaporin 4 antibody [4/18] (ab9512 ...
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bioRxiv - Microbiology 2019Quote: ... 25 µl of the extract was spotted (2 µl/transfer) onto a reverse-phase thin layer chromatography silica gel 60 RP-18 sheet (Merck, Kenilworth, NJ, USA). In addition to the LC510 AHL extract ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Microbiology 2022Quote: The His6-eVP40-pET46 expression vector was a kind gift from Erica Ollmann Saphire (La Jolla Institute for Immunology) and was expressed and grown in RosettaTM 2 BL21 DE3 competent cells (Merck Millipore, Billerica MA). The pet28a-His6-Lact C2 bacterial expression plasmid was a kind gift from Dr ...
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Microbiology 2022Quote: ... were sequentially filtered by vacuum through hydrophilic polycarbonate filters with a pore size of 20 µm (47 mm; Sterlitech, Kent, WA, US) and then 2 µm (Isopore, 47 mm; Merck Millipore, Cork, Ireland). Filters were immediately plunged into liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Cell Biology 2019Quote: ... The percentage of COX-2 positive cells was quantified on 5.103 dissociated UC-hMSCs using a Guava® easyCyte Flow Cytometer (Merck Millipore, Guyancourt, France). The results were compared to the fluorescent intensity distribution obtained by image analysis.
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bioRxiv - Microbiology 2019Quote: ... TEER was evaluated with the Millicell ERS-2 Voltohmmeter at the beginning and at the end of the assay (Merck-Millipore, Overijse, Belgium).
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Microbiology 2022Quote: ... medium was added to the apical side 30min prior to measurement with a chopstick electrode connected to a Volt-Ohm-meter (Millicell® ERS-2, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... injections 3x a week (consecutive days) during 2 weeks of 25 mg/kg 3PO or 75 mg/kg 3PO or dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Merck, Overijse, Belgium) as control ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit (Merck) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The DNA was extracted using a DNA purification kit (Merck Millipore, Darmstadt, Germany). Anti-RNA polymerase II antibody and normal mouse IgG were used as specific antibodies and the negative control ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA was extracted using GenElute™ Mammalian Genomic DNA Miniprep Kit (Merck), following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... The nitrate content was measured using Spectroquant Nitrate Test Kit HC707906 (Merck, Germany) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Blots were developed with the ECL kit (Thermo Fischer Scientific or Merck Millipore) and x-ray film and quantified using ImageLab.
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bioRxiv - Cell Biology 2020Quote: ... Blots were developed with the ECL kit (Thermo Fischer Scientific or Merck Millipore) and x-ray films.
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Gram staining of bacterial strains was performed using a Gram staining kit (Merck). Strain Ha5T cultured in LMG medium 404 broth for 24 hours was determined for cellular morphology using scanning electron microscope (FEI Quanta FEG 650) ...
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bioRxiv - Microbiology 2022Quote: ... Peptide content in each fraction was verified using QuantiPro BCA Assay Kit (Merck). Zn content was determined using PAR against a standard curve.
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bioRxiv - Cancer Biology 2022Quote: ... which was performed using the EZ-Nuclear RIP Kit (17-701, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: SecA ATPase activity assay was done using malachite green kit (MAK307, Merck/Sigma). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... the cytoskeleton) were extracted using the “ProteoExtract Subcellular Proteome Extraction kit” (Calbiochem/ MERCK) according to the supplier’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The PLA was performed using the Duolink Proximity Ligation Assay Kit Red (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... aeruginosa NCTC 11442 was extracted using the GenElute Bacterial Genomic DNA kit (Merck). Plasmid backbones and inserts for cloning were amplified from the purified chromosomal DNA using Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally embedded in epoxy resin (Epoxy embedding medium kit, Merck KGaA) that was then baked for 24 hours at 60 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... enhanced chemiluminescence assays were performed using the Millipore kit (Merck-Millipore, Stockholm, Sweden). The antibodies used are listed in the Supplementary Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were pre-stained with the PKH67 Green Fluorescent Cell Linker Kit (Merck) as a ground truth label ...
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bioRxiv - Neuroscience 2023Quote: ... Active (acylated) ghrelin concentrations were determined using the EZGRA-88K kit (Merck, Germany) in duplicates (total analytical CV at 488 pg/ml 12%).
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bioRxiv - Cell Biology 2023Quote: Saporin was labelled with an ATTO 550 protein labelling kit (Merck, 51146-1KT).
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bioRxiv - Neuroscience 2023Quote: ... Active (acylated) ghrelin concentrations were determined using the EZGRA-88K kit (Merck, Germany) in duplicates (total analytical CV at 488 pg/ml 12%).
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bioRxiv - Microbiology 2024Quote: The ATP was measured using the Roche ATP Bioluminescent HSII kit (Merck, Germany) as described by Nakagawa et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA extraction was performed using SPECTRUM(TM) PLANT TOTAL RNA KIT from Merck Catalogue #STRN50-1KT and eluted in water ...
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bioRxiv - Cell Biology 2024Quote: Rac1-GTP assay was performed using Non-radioactive Rac1 Activation Assay Kit (Merck) following manufacturer’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... the washed seedlings were transferred into TetSee X solution (15% Na-deoxycholate, 25% urea, 10 % glycerol, 5% 2’2-thiodiethanol [Merck, Product No. 166782], 1% Triton X-100) and kept for 3 days at 4°C with daily changes of the TetSee X solution ...