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Citations for Merck :
3051 - 3100 of 3939 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Neuroscience 2023Quote: ... they were washed 3 times with 1X PBS for 5 minutes and incubated with DAPI (4’6-Diamidino-2-phenylindole; 1 µg/mL, MERCK) for 5 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes were labeled before experiments with 1% of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhodamine) (Merck) at a concentration of 10µg/mL.
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bioRxiv - Cell Biology 2023Quote: ... including in an additional experiment where 1 mM of membrane permeable ATP (ATP disodium salt hydrate [A2383 – Merck, Australia]) was added in an attempt to rescue motility ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Biophysics 2023Quote: ... 5.5 µL of fibril sample was mixed with 0.5 µL of 1 mg/mL pronase protease (53702, Merck-Millipore) followed by adsorbing onto carbon-coated copper grids ...
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bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
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bioRxiv - Cancer Biology 2024Quote: The following primary antibodies and dilutions were used: mouse-anti-γH2AXSer139 1:1,000 (#05-636, clone JBW301, Merck Millipore) and rabbit-anti-53BP1 1:1,000 (NB#100-304 ...
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bioRxiv - Microbiology 2024Quote: ... Mouse mAb anti-HA (clone HA-7) and mouse anti-tubulin (clone B5-1-12) were purchased from Merck. Mouse mAb anti-V5 (SV5-PK1 ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed by resuspension in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.2% IGEPAL® [Merck]) and pelleted again in the magnetic rack ...
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bioRxiv - Plant Biology 2024Quote: ... the buffer was replaced with complex IV staining solution [1 mg/mL DAB (3,3′-Diaminobenzidine tetrahydrochloride hydrate; Merck D5637), 1 mg/mL cytochrome c (Merck C7752)] in complex IV buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained residue was derivatized with N-trimethylsilylimidazole/ trimethylchlorosilane reagent (TMSI:TMCS, 99:1, v/v; 50uL; both from Merck) and pyridine (50uL ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibodies against γH2AX (the same antibody used for wester blotting) and 53BP1 (1:1000, MAB3802 Merck). Cells were washed and incubated with secondary anti-rabbit-Alexa Fluor 488 (1:500 ...
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bioRxiv - Plant Biology 2024Quote: ... 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 2% w/v PVPP, 10 mM DTT, 0.2% IGEPAL® [Merck]) supplemented with EDTA-free protease inhibitor tablets (Roche) ...
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bioRxiv - Microbiology 2024Quote: “Swim” agar plates were made by pouring 0.3% LB agar into petri dishes with a 1-50 numbered grid sticker on them (Merck). Ciprofloxacin was added from a stock solution to each plate to a final concentration of either 0.015 μg mL-1 ...
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bioRxiv - Neuroscience 2024Quote: ... 10 worms from each RNAi treatment group were collected and cleaned in H2O before depositing in 1 μL of lysis buffer (0.25 mM EDTA (Merck), 5mM Tris pH 8.0 (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... NPCs were thawed in STEMdif Neural Induction Medium + SMADi on 0.01% Poly-L-ornithine/10-20 µg ml−1 laminin-coated (MERCK) cell culture plates and differentiated using the STEMdiff Forebrain Neuron Differentiation Kit (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.2 U μl−1 SUPERaseIn and 0.1% Triton X-100 in nuclease-free water) using a glass Dounce tissue grinder set (Merck). Samples were dissociated with 10-20 strokes of a loose pestle ‘A’ followed by 10 strokes of a tight pestle ‘B’ when tissue fragments remained ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were preincubated for 1 h at RT in blocking buffer: 5% normal donkey serum (NDS, Vector) and 0.2 % Triton X-100 (Merck) diluted in PBS ...
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bioRxiv - Zoology 2024Quote: ... Fish were anesthetized with MS-222 (0.1 g l-1 of sea water) (Sigma-Aldrich; Merck group, Darmstadt, DE) before each sampling and injection procedure ...
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bioRxiv - Immunology 2024Quote: ... Labeling efficiency was determined by ITLC using Whatman no.1 strips as solid phase and ethyl acetate (109623, Merck) as running buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet from a 3 l culture was resuspended in 80 ml TE pH 7.5 buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA) supplemented with Complete EDTA-free protease inhibitor cocktail (Merck) and 2.5 U ml−1 benzonase until sample was homogenous ...
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bioRxiv - Microbiology 2024Quote: ... The lipid film was hydrated with 1 ml of buffer A (10 mM HEPES pH 7.4, 150 mM KCl) containing 300 µM calcein (Merck), by vortexing in the presence of a 3 mm glass pearl ...
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bioRxiv - Microbiology 2024Quote: ... Micrococcin P1 was produced as described previously and solubilized to a concentration of 10 mg/ml in dimethyl sulfoxide (Sigma-Aldrich).30 Nisin A was obtained as part of a commercial fermentate which was solubilized to a concentration of 1 mg/ml in a 0.05% acetic acid solution (Merck). AuresinePlus was solubilized to a stock concentration 1 mg/ml in a buffer containing 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8) and permeabilized and blocked with 1X TBS supplemented with 1% Triton-X100 (MERCK; Cat #9036-19-5) and 6% FBS (Biological Industries ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... Both input and immunoprecipitated samples were separated by 12% SDS-PAGE and detected using anti-GFP (1:2000, Merck) and anti-Myc (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... DMSO (0.1%; D4540), L-glutamic acid (L-glutamate, 1 mM; G1251) and Sodium Propionate (5mM; P1880) were obtained from Merck Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... After heat induced antigen retrieval (Citrate buffer, pH6) of samples an anit-TSP1 antibody (1:400, Merck/Millipore MABT879) was diluted in blocking solution and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... SB431542 and LDN193189 were added until day 7 when cultures were supplemented with 1 μM smoothened agonist (SAG; Merck) and 0.5 μM purmorphamine (Pur ...
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bioRxiv - Biochemistry 2024Quote: The monomeric recombinant peptide was prepared by resuspending it in a solution of 1% NH4OH (Merck Cat. No. 221228) in PBS buffer at a concentration of 1 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... GSLs solubilized in chloroform/methanol (2:1, v/v) were applied to HPTLC plates (Kieselgel 60, Merck, Darmstadt, Germany) and developed in chloroform/methanol/water (55:45:9 ...
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bioRxiv - Cell Biology 2024Quote: ... depending on experiment (usually 104 cells/cm2 for three-day differentiation) in medium supplemented with 1 μM RA (Merck) and deprived of LIF for indicated periods of time ...
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bioRxiv - Cell Biology 2024Quote: ... One of them was an anti-phospho-histone γH2A.X antibody (Cell Signalling Technology, Danvers, United State; 1:250) and the second was anti-NeuN (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellets (1.5 x 106 cells) were lysed with 150 µL of 1× RIPA buffer (Merck Millipore), kept on ice ...
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bioRxiv - Genetics 2024Quote: ... Then 1 μl of the digest RNA sample was spotted on cellulose F TLC plates (20 × 20 cm, Merck) and run for the first dimension in a solvent system of iso-butyric acid:NH4OH(28-32% ...
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bioRxiv - Cell Biology 2024Quote: ... Fabs were concentrated up to more than 1 mg/mL using 10 k cut-off filters (Amicon Ultra-0.5 10 k; Merck), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: Blocks of cells or tissue were incubated overnight in 2.3M sucrose at 4°C (Merck, K17687153) in 0.1M phosphate buffer ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were detached in a similar manner: 4 mg/mL lidocaine hydrochloride (L5647; Merck KGaA) were solved in Versene Solution freshly for each detachment ...
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bioRxiv - Neuroscience 2021Quote: ... Stained sections were washed 4 times in PBS and then embedded using Aquatex (Merck Millipore, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... the medium was removed and cells were fixed for 10 min in 4% formaldehyde solution (Merck). The fixation solution was removed and the cells were washed twice with nuclease-free H2O (nfH2O) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with 0.1% SDS (Merck Millipore, Burlington, MA). Primary antibodies were incubated for 1 h at RT a humid chamber ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of each sample were concentrated with Amicon ultra Centrifugal filters (Cat no. UFC510024, Merck). Samples were centrifuged (14 000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated with Amicon Ultra-4 Centrifugal Filter Unit (100 kDa cut-off, Merck Millipore, Cat#UFC810024) to ~1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting ultrafiltrate (15min 14000g 15°C) was acidified with 20 µl 4% formic acid (Merck) in dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-integrin β1 antibody (clone HUTS-4) was obtained from Chemicon (Merck Millipore, Billerica, MA). Rabbit polyclonal antibodies to fibronectin and fibrinogen were from Abcam (Cambridge ...