Labshake search
Citations for Merck :
3001 - 3050 of 3327 citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... along with the amino ligand to be coupled, in our case melittin (5 mg/ml, Genescript, USA) and Bovine serum albumin (BSA) (1 mg/ml, Merck) was used per bead ...
-
bioRxiv - Molecular Biology 2024Quote: Coverslips were transferred to a humid chamber and incubated overnight with the primary antibody diluted in 1% BSA/PBS (anti-MAP2 antibody (AB554, Merck), 1/500 dilution ...
-
bioRxiv - Developmental Biology 2024Quote: ... in PBS for 30 minutes and washed twice in PBS for 1 minute each prior to permeabilization: sections were briefly digested with 0.5 mg/ml pepsin (Merck P7012) in 0.1 M HCl (Fisher 10325710 ...
-
bioRxiv - Microbiology 2024Quote: ... routine wash and control runs were done by injecting 5 µl LC-MS solution containing 5 µL of 10 fmol/µL BSA or enolase digest and 1 µL of 10 fmol/µL angiotensin III (Fluka, Thermo)/oxytocin (Merck) to check the performance of the platform on each component (nano-LC ...
-
bioRxiv - Microbiology 2024Quote: ... LS-ARS2 overnight culture was inoculated (1% v/v) in MRS broth adjusted to pH 4 and pH 3 with HCl (1N, Merck), followed by incubation for 0 ...
-
bioRxiv - Immunology 2024Quote: ... Monocytes were plated in serum-free RPMI containing 10 mM sodium pyruvate for 1 hr prior to changing media to complete RPMI supplemented with 10% human serum (from human male AB plasma, Merck), 1 U/mL penicillin and 0.1 mg/mL streptomycin ...
-
bioRxiv - Molecular Biology 2024Quote: ... cell pellets were resuspended in 500 μL Nuclear RNA lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% BSA (Merck, #B9000S), 0.1% Tween (Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... each sample was pelleted by centrifugation at 3,000 g for 10 min at 4°C and the pellet was resuspended in 100 μl of 1% SDS lysis buffer (1% SDS in 50 mM Tris-HCl (pH=8.0) and supplemented with 1X protease inhibitor cocktail (from 100X; Merck, P8849), 1 mM phenylmethylsulfonyl fluoride (PMSF ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 (PBST)) for 10 minutes and blocked for 1 hour with 10% normal donkey serum (Merck Millipore), 1% bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2024Quote: ... 130 pg of gRNA and 250 pg of the Cas9 protein were co-injected into a 1-cell stage fertilized embryos of AB WT fish with phenol red (pn. 114537-5G, Merck) as a tracer dye ...
-
bioRxiv - Biochemistry 2024Quote: ... and concentrated to ∼ 10 mg/mL (as judged by the extinction coefficient χ410= 213 mM-1cm-1) using Amicon 50K MWCO centrifugal concentrators (Merck). Dimeric NmqNOR was immediately used to make grids following SEC ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
-
bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
-
bioRxiv - Microbiology 2024Quote: ... Peak fractions were concentrated to 1.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by an incubation overnight at 4 °C in blocking solution with anti-puromycin antibody (clone 12D10, MABE343; 1:500) AlexaFluor 488 or AlexaFluor 647 (MABE343-AF488 or MABE343-AF647; Merck). On the following day ...
-
bioRxiv - Neuroscience 2024Quote: ... Medium change was performed every 72 h by exchanging half of the volume of the medium with fresh NB medium with complements and AraC (1 μg/ml for the first, and 0.5 μg/ml for the second medium change, Sigma/Merck, Germany), cells were harvested at DIV7 (ChIPseq ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Zn and Mn extraction from earthworm tissue was performed using Teflon vessels and 7 mL of reverse aqua regia (3:1 ratio of HCl:HNO3; Merck, Darmstadt) on hot plates in an open system ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated at 4°C (48h) with the rabbit polyclonal primary antibody anti-TH (1:1000, Merck, Germany, #AB152), rinsed three times in 0.1 M PB ...
-
bioRxiv - Physiology 2024Quote: ... followed by 7 days treatment with primary antibodies diluted 1:10,000 in 100 ml immunostaining buffer (3% horse serum, 10% CHAPS (Merck, 220201), 2% Triton X-100 ...
-
bioRxiv - Biochemistry 2024Quote: Peak fractions from size-exclusion chromatography were concentrated to 4-6 mg mL-1 using a 50 kDa MWCO spin column (Merck), followed by addition of 30 mM D-glucose ...
-
bioRxiv - Systems Biology 2024Quote: ... rehydrated sections [20] in a 800 ml glass container filled with the retrieval solutions (EDTA pH 8; 1 mM EDTA in 10 mM Tris-buffer pH 8, Merck Life Science S.r.l.,Milano ...
-
bioRxiv - Cancer Biology 2024Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies anti-acetylated tubulin (Merck, T7451, 1/2000); anti-Arl13b (Sanbio ...
-
bioRxiv - Biophysics 2024Quote: ... the cells were incubated overnight at 4°C with the primary antibodies: rabbit anti-NFH (1:1000, Merck millipore, Germany) and mouse anti-SMI312 (1:250 ...
-
bioRxiv - Bioengineering 2024Quote: ... and seeded on four 60 mm dishes (3010-060, AGC Techno Glass) precoated with 1 µg/cm2 fibronectin (FC010, Merck) and cultured for 2 days in StemPro-34 SFM with 2 mM GlutaMAX ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 μL of this complex was added to a mixture of 15 μL of TIRF-Casein buffer supplemented with 1 μL each of an oxygen scavenging system (4 mg/mL catalase, Merck; and 30 mg/mL glucose oxidase ...
-
bioRxiv - Cell Biology 2024Quote: ... they were incubated for 1 hour at RT with a blocking solution that consisted of 5% chemiBLOCKER (Merck, Darmstadt, Germany) in 0.1% PBS Triton X-100 ...
-
bioRxiv - Bioengineering 2024Quote: Fresh macroscopically normal omental and peritoneal tissues were cut into 1-2 mm3 pieces and placed into non-adherent U-bottom 96 well plates (Merck). A solution with 10,000 cells in 10 μl media was added and cells were allowed to attach to the tissues for 2 h at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... EV-enriched fractions (1-4) were pooled and concentrated using Amicon 15 Ultra RC 10 kDa MWCO filters (Merck Millipore). Expi293F-palmGRET EV aliquots in Dulbecco’s phosphate buffered saline (DPBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... routine wash and control runs were done by injecting 5 µl LC-MS solution containing 5 µL of 10 fmol/µL BSA or enolase digest and 1 µL of 10 fmol/µL angiotensin III (Fluka, Thermo)/oxytocin (Merck) to check the performance of the platform on each component (nano-LC ...
-
bioRxiv - Cell Biology 2024Quote: ... organoid culture medium is removed and replaced by fresh medium containing the fluorescent probes to stain the DNA (Hoechst, 1:3000 v/v) and the tubulin (Biotracker 488 Microtubules, Merck, dilution 1:500 used with Verapamil 1:1000 to contain the probe inside the cells).
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were then incubated for 1 hour in 10 ml of 80% THF/H2O and twice 1 hour in 100% THF and then in dichloromethane (DCM) (270997-12X100ML, Merck) until they sank at the bottom of the vial ...
-
bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
-
bioRxiv - Biophysics 2020Quote: GMPCPP-stabilized microtubules were grown using a mixture of 1.3 mg ml−1 tubulin and 2 mM GMPCPP (Merck, NU-405L) in BRB80 and incubated for 1 hour (stepping and photobleaching assay ...
-
bioRxiv - Cell Biology 2020Quote: ... All supernatant was removed using a 1 ml syringe and the beads were resuspended in 30 μl 2X SDS Sample Buffer (Merck Millipore). The samples were incubated at 98°C for 5 minutes before electrophoresis.
-
bioRxiv - Developmental Biology 2021Quote: ... Lymphocytes were isolated from the spleen and incubated in a tissue culture tube at a cell concentration of 1 × 106/ml in RPMI1640 (Nacalai Tesque) containing lipopolysaccharide (10 μg/ml, Merck KGaA), concanavalin A (3 μg/ml ...
-
bioRxiv - Molecular Biology 2021Quote: ... The chromatin pellet was digested in 100 μl of nuclease-free water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 minutes at 37°C in a thermomixer at 1,400 rpm ...
-
bioRxiv - Developmental Biology 2021Quote: ... the following antibodies were treated to the organoids and the tissue slices at 4°C overnight: proSFTPC (1:200; Merck, AB3786), E-cadherin (1 ...
-
bioRxiv - Cell Biology 2022Quote: ... The solution consisted of collagenase type I (1 mg/ml) prepared in Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12, Sigma Aldrich/Merck, Poznan, Poland). The tissue fragments were digested for 40 minutes in CO2 incubator at 37 °C with periodic vortexing ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were lysed using ice-cold RIPA buffer containing 1% of protease and phosphatase inhibitor cocktail (Sigma Aldrich/Merck, Poznan, Polska). The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck ...
-
bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
-
bioRxiv - Biophysics 2020Quote: ... dialyzed against Milli-Q water and concentrated to 4 mg ml-1 using Amicon Ultra centrifugal filter units with Ultracel-50K (Merck Millipore). UMODe filaments were obtained by limited digestion of UMODfl with elastase (Jovine et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were fixed and stained according to the procedure described above using primary antibodies against NESTIN (Merck, ABD69, 1:300 dilution) and TUBB3 (Santa Cruz ...
-
bioRxiv - Neuroscience 2021Quote: ... The brains were removed and post-fixed for 1-2 days and then transferred to 30% sucrose (Merck KGaA, Darmstadt, Germany) dissolved in PBS ...
-
bioRxiv - Biophysics 2021Quote: ... and concentrated to >1.5 mg/mL.The DNA and antibody solutions were cross-reacted at a 10:1 molar ratio overnight and excess DNA was filtered through a 100 kDa Amicon spin column (UFC510096, Merck Milipore). The antibody-DNA solution was stored at 4°C.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
-
bioRxiv - Plant Biology 2021Quote: ... The eluate containing the nanodiscs was concentrated to 1 ml with the use of an Amicon Ultra-15 10K device (Merck Millipore) and then mixed with 10 ml of translation buffer containing 30 mM HEPES-KOH (pH 7.8 ...
-
bioRxiv - Biochemistry 2021Quote: ... truncatula leaf tissue was ground in liquid nitrogen and extracted three times in 1 ml of 80% HPLC-grade methanol (Merck, Germany). Each time the mixture was sonicated for 15 min at room temperature and centrifuged for 10 min at 15,000 ×g ...