Labshake search
Citations for Merck :
251 - 300 of 910 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The sample was buffer exchanged into TNE buffer by serial dilution and concentration using Amicon Ultra centrifugal filters (Merck).
-
bioRxiv - Biochemistry 2023Quote: ... Cells were washed twice in 40 µL/well flow buffer and resuspended in 40 µL/well flow buffer with 2.5 µg/mL DAPI (Merck). The median fluorescence intensity of living single cells was determined in triplicates using a ZE5 Cell Analyzer (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... bound secondary antibodies were detected using either EZ-Chemiluminescence Reagent (Geneflow Ltd) or Luminata™ Forte Western HRP Substrate (Merck Millipore) and a Biospectrum 500 imaging system (UVP ...
-
bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
-
bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... and detected using primary and corresponding HRP-conjugated secondary antibodies on Fusion SL imaging system (Vibler) using Immobilon Western Chemiluminescent HRP Substrate (Merck, WBKLS0500). Molecular size of bands is marked in each panel [kDa] ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour with a species-appropriate HRP-conjugated secondary antibody (diluted 1:5000 in TBST) before bands were visualised using Immobilon Forte Western HRP Substrate (Merck Millipore). Primary antibodies used were mouse anti-αSMA (Dako ...
-
bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with goat anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody and the bands developed with Luminata Forte Western HRP Substrate (Merck Millipore).
-
bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed 3x in TBST for 10 min again and afterwards incubated in Immobilon ECL Ultra Western HRP Substrate (Merck, WBULS0100) for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
-
bioRxiv - Plant Biology 2022Quote: ... Secondary antibody and detection procedure was carried out following the manufacturer’s instructions (ECL™ Prime Western Blotting System, Merck; Darmstadt, Germany).
-
bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
-
bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... The signal was visualized using appropriate primary and secondary antibodies and the Immobilon™ Western Chemiluminescent HRP substrate (Merck, Darmstadt, Germany) and developed on an X-ray film ...
-
bioRxiv - Cell Biology 2020Quote: 1×106 wt and hem1-/- DCs were spun down and lysed in 50µl 1x lysis buffer (1ml 10x RIPA buffer - NEB, 9ml H2O, 1 tablet PhosStop and 1 tablet protease inhibitor mini - both Merck). Lysates were spun at full speed for 10min at 4°C and supernatants transferred to new tubes ...
-
bioRxiv - Molecular Biology 2019Quote: ... The proteins were again diluted in 9 volumes of Exchange Buffer 2 (Exchange Buffer 1 with 200 mM NaCl) and concentrated with 30 MWCO Amicon Ultra-15 (Merck).
-
bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore ...
-
bioRxiv - Microbiology 2023Quote: ... were pre-prepared by washing 3 times in immunoprecipitation buffer then resuspended in 200μl immunoprecipitation buffer and coated with 2 μl FLAG M2 (Merck Millipore) or IgG (Merck Millipore ...
-
bioRxiv - Biophysics 2023Quote: ... in Tyrode buffer lacking Ca2+ (to reduce drastically both exo-and endocytosis; the Tyrode buffer contained 124 mM NaCl (#K52190904041, Merck), 5 mM KCl ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was removed by placing samples on a magnetic stand, the beads were resuspended in 100 μL of Antibody buffer (Wash buffer, 0.025% Digitonin (Merck, 300410), EDTA 2 mM ...
-
bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and post-fixed in 17 % sucrose buffer (Merck) containing 1 % OsO4 (Roth ...
-
bioRxiv - Molecular Biology 2020Quote: ... dropwise to HBS buffer (Sigma-Aldrich; Merck KGaA). This transfection complex was added dropwise to 7080% confluent cells then the cells were incubated at 37°C for 8 hours ...
-
bioRxiv - Microbiology 2020Quote: ... Loading buffer was 0.1% trifluoroacetic acid (Merck Millipore) in water ...
-
bioRxiv - Physiology 2019Quote: ... in 0.1M Na-cacodylate-buffer (Merck, Darmstadt, Germany) with a pH of 7.33 ...
-
bioRxiv - Microbiology 2020Quote: ... RIPA buffer (no. R0278; Sigma Aldrich-Merck, USA), Hypoxanthine ...
-
bioRxiv - Neuroscience 2022Quote: ... in tris-borate-EDTA buffer 1X (T4415, Merck). Cubes of 1 cm3 were cut and incubated overnight in an aqueous solution of 150 mM NaCl (S9888 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 μl of PopCulture lysis buffer (Merck Millipore) was added to each well ...
-
bioRxiv - Bioengineering 2023Quote: Cells were lysed with RIPA buffer (Merck Millipore) containing 1% protease and phosphatase inhibitor cocktails on ice ...
-
bioRxiv - Immunology 2023Quote: ... and resuspended in 1X RIPA buffer (Merck Millipore) containing protease inhibitor cocktail (Roche) ...
-
bioRxiv - Biochemistry 2023Quote: Buffers were prepared using MilliQ water (Millipore, Merck). Glycine hydrochloride buffer ...
-
bioRxiv - Bioengineering 2023Quote: ... 100 µl of 1x RIPA lysis buffer (Merck) supplemented with 10 % glycerol and a proteasome inhibitor (Roche ...
-
bioRxiv - Genomics 2023Quote: ... and buffers were purchased from Merck (Darmstadt, Germany), Sisco Research Laboratories Pvt Ltd (Mumbai ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 40 μL RIPA buffer 1X (Merck, UK) with added cOmplete ULTRA protease inhibitors (Roche ...
-
bioRxiv - Neuroscience 2024Quote: ... buffer and amino acids were obtained from Merck| Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: Buffers contains cycloheximide (10 µg/mL, Merck, C7698) at each step of this protocol ...
-
bioRxiv - Biophysics 2021Quote: ... Eluted VWF constructs were buffer exchanged (to the running buffer) and concentrated by centrifuge filtration using Amicon UltraMWCO 100 kDa (Merck Millipore). After 5 washing steps with 500 μl of running buffer ...
-
bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged GB1 and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to HEPES buffer containing 40 mM imidazole using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S75 Increase 10/300 column equilibrated in HEPES buffer without imidazole and a pH of 7.5 ...
-
bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged Mistics and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to Tris buffer containing 40 mM imidazole in an Amicon centrifugal filter (Merck Millipore) with adequate cutoff ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleaved His-tagged GB1 and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to Buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... This was followed by the removal of the buffer by placing the microtube in a magnetic rack and three times washing with IP buffer.200 μl IP buffer wasalong with fragmented RNA and 10 μl RNAse Inhibitor (Merck #CS216138) was then added to the antibody coupled to beads ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.5 ml nuclei wash, and suspension buffer (NWS-Buffer: 2% BSA, 2 mM MgCl2, 0.5% Protector RNAse Inhibitor (SKU3335399001, Merck, Soeborg, Denmark)) was carefully added ...
-
bioRxiv - Biochemistry 2024Quote: ... Cleaved His-tagged GB1 fragment and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex 200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... The next day cells were lysed in 200 µl of PEG switch sample buffer (PS buffer) (1% SDS, 50 mM HEPES) supplemented with 100 mM NEM (Merck, E3876), 1 mM PMSF (Merck ...
-
bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... Blots were then reprobed with monoclonal anti-β-actin-peroxidase clone AC-15 antibody (a3854, Merck, Saint Louis, MO, dilution 1:5000) in TBS/T for 90 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
-
bioRxiv - Biochemistry 2020Quote: ... Pooled fractions were buffer exchanged to buffer A and concentrated by ultrafiltration on 3000 MWCO Amicon filter units (Millipore Merck, Darmstadt Germany). A final concentration of 10.2 mg.mL-1 was measured by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...