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Citations for Merck :
251 - 300 of 3905 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from cells or tissues by RIPA buffer supplemented with a protease inhibitor cocktail (Merck, #11836170001) and phenylmethanesulfonyl fluoride (Beyotime ...
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bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Biochemistry 2020Quote: ... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
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bioRxiv - Cancer Biology 2022Quote: PEGylated-poly T coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 minutes followed by wash with IMB ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 5 times with PBS-T and 100µl of 3,3′,5,5′-Tetramethylbenzidine (TMB) (Merck Millipore) equilibrated at room temperature were added ...
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bioRxiv - Cancer Biology 2023Quote: PEGylated-poly(T)-coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell lysates were transferred to a 0.45 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore), centrifuged for 5 min at 500 xg and 4°C to remove aggregates and collected in a 96 well polypropylene plate ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated O/N at 4 °C with the following primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP; Merck, Madrid, Spain; #MAB360; 1:1000) and rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with directly conjugated fluorescent antibodies in PBS/1% (w/v) BSA (Merck)/0.05% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 400 μl cell lysate were incubated with 4 μl HTT MAB2166 rabbit primary antibody (Merck, #MAB2166) for 2 hours at 4° C on a rotating device ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), Collagen I (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... neural cells were stained with rabbit polyclonal anti-tyrosine hydroxylase antibody (Merck, AB152, 1:200 dilution), guinea pig polyclonal anti-DCX antibody (Merck ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...
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bioRxiv - Physiology 2024Quote: Total protein carbonylation was detected by immunoblotting using the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was first incubated for 1 h in 50 mL 5% [w/v] semi-skimmed milk in TBS-T (150 mM NaCl, 20 mM Tris/HCl pH 7.6, 0.05%[v/v] Tween 20 (Merck) at room temperature (RT) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
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bioRxiv - Immunology 2024Quote: ... plates were washed in PBS/T and incubated for 1 h with goat anti-mouse IgG-AP (Merck). Plates were then developed as per the mAb ELISA ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 1XPBS + 0,1% Tween (1XPBS-T) with non-fat dry milk (5%, Merck Millipore) for 30 minutes at RT and then incubated for 3 hours at RT with a primary antibody resuspended in 1XPBS-T with 5% milk ...
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bioRxiv - Bioengineering 2024Quote: ... the membranes were washed three times for 10 min in PBS-T (PBS with 0.1% Tween-20, Merck). Then ...
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2024Quote: Plasmids used for protein expression and purification (listed in S3 Table) were transformed into Escherichia coli Rosetta (DE3) cells (Merck Chemicals). A single positive colony of fresh E ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
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bioRxiv - Neuroscience 2022Quote: ... and one of the following primary antibodies: mouse anti-GAD67 for staining GABAergic cells (Merck & Co., MAB5406), goat anti-Substance P for staining D1-containing cells (Santa Cruz ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... cells were incubated with an IF-optimized EMRE antibody (anti-SMDT1/C22orf32/EMRE; #HPA060340, 1:250; Merck), washed 3 times in PBS ...
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bioRxiv - Microbiology 2024Quote: ... cells were secondary labelled with a fluorescein isothiocyanate (FITC) conjugated goat anti-mouse IgG antibody (F5897, Merck). Cells were fixed with 1% paraformaldehyde ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking buffer before the addition of secondary antibody and DAPI (Merck) for 45 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... yeast cells were resuspended in 1:10-diluted primary mouse anti-cMyc antibody (#OP10, EMD Millipore, Merck) in PBS (50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...