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Citations for Merck :
251 - 300 of 1295 citations for Recombinant Human Interleukin 22 Receptor Alpha 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% aprotinin and 0.1% Human Insulin Solution (Merck). Medium was changed every other day with EHT medium.
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HTF medium (EmbryoMax Human Tubal Fluid; Merck Millipore) was mixed 1:1 with mineral oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal human epidermal keratinocytes were obtained from Merck. All lines were maintained in 3:1 DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... growth in LB and human serum (Merck #H4522) was measured ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (all Merck). The pH was adjusted to 7.3-7.4 (S20 ...
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bioRxiv - Biophysics 2023Quote: ... 2-aminoethoxydiphenyl borate (2-APB) (Sigma-Aldrich/Merck, Germany), BL-1249 ...
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 1000 units/ml of recombinant Leukemia Inhibitory Factor (LIF, Interchim, #8V6280) and 0.1% β-mercaptoethanol (Merck, #M6250) on gelatin-coated plates ...
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bioRxiv - Cell Biology 2019Quote: ... 2% peptone (Becton Dickinson) and 2% glucose (Merck, Darmstadt, Germany). SC consisted of 0.7% yeast nitrogen base (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 2% peptone (Becton Dickinson) and 2% glucose (Merck, Darmstadt, Germany). SCD-MSG medium consisted of 0.17% yeast nitrogen base without amino acids and ammonium sulfate (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Biophysics 2021Quote: ... Human pancreatic beta cells 1.4E7 were purchased from Merck and cultured in RPMI-1640 medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... conidia were opsonized with normal human serum (Merck Millipore). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... human mitochondria (clone 113-1, Merck Millipore, 1:100), and BrdU (clone BU1/75 ICR1 ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml human EGF (Merck, Cat Number: E9644), 0.5 mg/ml hydrocortisone (Merck ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg/mL mouse anti-human TGN46 (SAB4200355, Merck), or a 1 in 20 dilution of rat anti-β-tubulin (clone YL1/2 ...
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bioRxiv - Biophysics 2021Quote: Rabbit polyclonal anti human IRSp53 (07-786 – Merck Millipore), mouse monoclonal anti CA (24.2 – NIH AIDS Reagent Program – FisherBioServices) ...
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bioRxiv - Biophysics 2021Quote: ... rabbit polyclonal anti human IRSp53 (07-786 – Merck Millipore), mouse anti CA (183H125C – NIH3537) ...
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bioRxiv - Bioengineering 2021Quote: The human immortal chondrocyte cell line – C28/I2 (Merck) was used in this study ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.2 IU/ml human chorionic gonadotropin (HCG) (C1063, Merck), 25 µg/ml sodium pyruvate and penicillin-streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg/ml of IgG from human serum (Merck) in PBS were added to all blocking and antibody incubations ...
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bioRxiv - Cell Biology 2023Quote: ... were coated with human plasma fibronectin (Merck Millipore FC010) at a concentration of 5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-human IgG (AP309P, Merck KGaA). For stripping and re-probing of Western blot membranes ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore), and anti-E-cadherin-1 (ECAD ...
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bioRxiv - Microbiology 2024Quote: ... the bacterial suspension and human serum (Merck, Darmstadt, Germany) were mixed in a 1:1 ratio (100 µL each) ...
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bioRxiv - Cell Biology 2024Quote: ... 50nM Mission esiRNA against human PTGER2 or PTGER4 (Merck). 50nM non-targeting Silencer™ Negative Control #1 siRNA (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Merck), JQ-1 (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Cell Biology 2023Quote: ... pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
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bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat. #A05174 and Human Adiponectin ELISA, Merck, Cat. # EZHADP-61K). The minimum detectable dose of leptin was 7.8 pg/mL and that of adiponectin was 0.891 μg/mL.
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... antigen retrieval and antibody staining (anti-PER2, Alpha Diagnostic, cat. PER21-A, 1:200 dilution; anti-GFAP, Abcam, cat. ab53554, 1:500 dilution; anti-NeuN, Merck Millipore, cat. mab377, 1:250 dilution). The procedures have been described by our laboratory in detail elsewhere (Brenna et al. ...
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bioRxiv - Immunology 2021Quote: ... Human pancreatic beta cell line 1.4E7 was purchased from Merck and cultured in RPMI-1640 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Part B was made of 10U/mL human thrombin (Merck) in DMEM/F12 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and purified human C3a was purchased from Merck (Perth, Australia). Recombinant human C5a (rhC5a ...
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bioRxiv - Immunology 2020Quote: ... FITC conjugated goat anti-human IgG Fc was from Merck, Dorset ...