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Citations for Merck :
251 - 300 of 753 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Cancer Biology 2021Quote: Urine samples were first adjusted to pH 8.0 using 500 mM ABC supplemented with EDTA-free protease inhibitor and then filtered through an Amicon Ultra-0.5 mL Centrifugal Filter Device (10 kDa, Merck Millipore). Collection of the retentate was performed by flipping the filter device upside-down and conducting centrifugation at 1,000g for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... after which the filtrate was filtered onto 0.2 µm pore size (free-living cells) polycarbonate filters (Merck Millipore, Darmstadt, Germany). Foam from the Timor Sea (Station 8 ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with ice-cold PBS and lysed for 5 min on ice in 50 μL RIPA buffer supplemented with protease inhibitors (Protease Inhibitor Cocktail set III, EDTA free, Merck) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Cell Biology 2020Quote: ... in ice-cold serum-free RPMI media (R5886, Merck, with 100 U/μg/ml penicillin/streptomycin [P/S, P0781, Merck] and 2 mM L-glutamine [L-glut ...
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bioRxiv - Evolutionary Biology 2023Quote: ... starting with a digestion of animal tissues form different life-stages in 500ml of Animal Free Collagenase/Dispase Blend I (Merck) dissolved in 35‰ FSASW (filter sterile artificial sea water ...
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Developmental Biology 2022Quote: ... The cloudy mass of sperms was pulled away from the epithelial layer using the needle and transferred into a 1.5 ml microfuge tube kept on the ice having 100 μl of 1X PBS containing 1X protease inhibitor cocktail (PIC) (cOmplete EDTA-free, Merck). The sperms isolated from 75-100 male flies were used for further processing.
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bioRxiv - Neuroscience 2022Quote: ... slide-mounted OE sections or free-floating OB slices were washed with PBS and incubated in 3 % BSA (Merck, A2153) with 0.25 % triton and 0.02 % sodium azide (Severn Biotech Ltd ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmin used for activation was not removed but inhibited using cOmplete™ EDTA-free Protease Inhibitor Cocktail (Merck, 04693132001) or Pefabloc® SC (CarlRoth ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... grown in semi-suspension in 150 mm plates in a mixture of serum free media (ACF CHO medium EX-CELL Merck, CHO ONE media Capricorn Scientific ...
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bioRxiv - Biochemistry 2024Quote: Milli-Q water (EQ7000 Ultrapure Water Purification System, Merck KGaA, Darmstadt, Germany) was used to prepare all aqueous solutions ...
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bioRxiv - Plant Biology 2020Quote: ... treated with RNase A (Merck), and DNA was precipitated with isopropanol at −20°C ...
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bioRxiv - Neuroscience 2020Quote: ... 20% water (Merck Millipore), and 50 pmol/ml of amino acid internal standard MIX AA (Cambridge) ...
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bioRxiv - Microbiology 2020Quote: ... Water (HPLC grade, Merck) was acidified to pH 3.2 using orthophosphoric acid ...
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bioRxiv - Biochemistry 2022Quote: ... and ultrapure water (Merck) at 70°C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... water (Lichrosolv®; Merck) and DNA template (1-2 μL of genomic DNA) ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Molecular Biology 2020Quote: ... all cell lines were seeded in black-walled 96 well plates at a density of 1×104 cells/well or and were maintained in the dark after induction of optogenetic receptor expression through 24 hours incubation in serum-free medium containing 100ng/ml doxycycline hyclate (Merck, #D9891). Optical stimulation was performed at room temperature using either a custom-built Arduino-controlled LED array device using 470nm LEDs (Ballister et al. ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III, EDTA-free, Merck/Sigma-Aldrich) supplemented with glycine and isolated by centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... pH 7.0) containing the cOmpleteTM EDTA-Free protease inhibitor at a 1 x concentration following manufactureŕs instructions (Merck Millipore, Darmstadt, Germany). The buffer volume was proportional to the cell density of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by approx ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were then washed in cold 50 mL cold PBS cOmplete™ EDTA-free Protease Inhibitor Cocktail tablets (Merck, #73567200) and centrifuged at 2000 rpm for a further 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Final polishing of pooled fractions was achieved by SEC on a Superdex 200 with on-column buffer exchange to endotoxin-free PBS (Merck Millipore) and then snap-frozen in liquid nitrogen and stored at -20 °C until use.
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bioRxiv - Cancer Biology 2024Quote: ... cell culture media was changed to FCS-free high glucose DMEM containing 12.5 µM 13C16-labelled or unlabelled palmitate conjugated to 2% fatty acid-free bovine serum albumin (BSA, Merck Life Sciences). Palmitate was prepared in cell culture media from 100 mM ethanolic stocks of palmitic acid and conjugated to fatty acid-free BSA by incubating at 55°C in media for 2 h ...
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bioRxiv - Biophysics 2024Quote: ... and resuspended in 1 mL PBS supplemented with protease inhibitor and protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... Medium in the capillary channel was then replaced with serum free endothelial cell media containing 40 mg/ml FITC-albumin (Merck, A9771), and the non-cellular channel medium replaced with serum-free RPMI ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNase I (Merck). Bacterial lysis was performed by sonication (QSonica ...
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bioRxiv - Microbiology 2023Quote: ... DNase I (4716728001, Merck), S1 (N5661-50KU ...
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bioRxiv - Biochemistry 2024Quote: ... and DNAse I (Merck). After lysate clearing by centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Cancer Biology 2024Quote: ... a concentrated lipid mixture stock solution of fatty acids conjugated to fatty acid-free BSA (10% solution in PBS) (Merck cat# A1595) was created as previously described71 ...
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bioRxiv - Cancer Biology 2024Quote: All cell lines were routinely (quarterly test intervals) confirmed to be free of mycoplasma infection using PCR LookOut® Mycoplasma PCR Detection Kit (Merck, UK).
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bioRxiv - Cell Biology 2024Quote: ... The cells were resuspended in 1 mL PBS containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck, #11836153001) (PBSI ...
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bioRxiv - Plant Biology 2020Quote: ... Conidia for either seeding liquid cultures or for use in pathogenicity assessments were collected by washing 4-week-old MM cultures with sterile water (SW, Milli-Q Integral Water Purification System for Ultrapure water, Merck KGaA, MA, USA), then filtered through glass wool to remove mycelial debris ...
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bioRxiv - Molecular Biology 2023Quote: A total of three water matrices were used in the experiment: Milli-Q tap water (from Independent Q-POD® ultrapure water dispensing unit (Merck, Darmstadt, Germany), water from Lake Zurich ...
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bioRxiv - Cancer Biology 2023Quote: ... Water was purified with a Milli-Q Simplicity 185 Water Purification System (Merck Millipore). All other chemicals and solvents obtained from commercial suppliers were used without further purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Water was purified with a Milli-Q Simplicity 185 Water Purification System (Merck Millipore). All other chemicals and solvents obtained from commercial suppliers were used without further purification ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μg/ml RNase A (Merck) was added ...
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bioRxiv - Cell Biology 2021Quote: ... with DEPC-treated water (Merck) in the water bath ...
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bioRxiv - Physiology 2022Quote: ... water and formic acid (Merck) in different proportions ...
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bioRxiv - Biochemistry 2022Quote: ... LC-MS grade water (Merck), and LC-MS grade acetonitrile (Merck).