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Citations for Merck :
251 - 300 of 2390 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Biochemistry 2022Quote: ... Using TLC plate aluminum foils (Silicagel 60, 20 × 20, Merck) which were cut to the desired size (minimum height of 10 cm) ...
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bioRxiv - Plant Biology 2020Quote: ... Analytical grade zinc acetate dehydrate (Zn(CH3COO)2.2H2O) and citric acid (C6H8O7) were purchased from Merck and used as received ...
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bioRxiv - Biochemistry 2021Quote: ... in centrifuge filters (Corning Costar Spin-X 0.45 um pore size cellulose acetate membrane, Merck, UK) following standard methods ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To each sample we added 375 μl of ammonium acetate buffer (NH4CH3COO-) 1M (Merck, Darmstadt, Germany), at PH=5 ...
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bioRxiv - Immunology 2024Quote: ... the cells were stimulated with 200 ng/ml phorbol 12-myristate 13-acetate (PMA; P1585; Merck), 100 ng/ml calcium ionomycin (CaI ...
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bioRxiv - Cancer Biology 2023Quote: ... 20% DMSO (Merck) in TBS (0.1M Tris ...
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bioRxiv - Neuroscience 2020Quote: ... half of the medium was replaced with fresh medium containing 20 μM of 5-fluorodeoxyuridine and uridine (Sigma Aldrich, Merck, Darmstadt, Germany) every 3 days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were pelleted (1500 RPM 5 min) and resuspended in PBS containing 20 µg/ml propium iodide and 200 µg/ml RNase (Merck Life science).
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... 3% casein (Merck) in 20 mM TBS (pH 11.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nutlin-3 (Merck) was dissolved in DMSO and diluted in saline.
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bioRxiv - Molecular Biology 2021Quote: ... membranes were washed 3 times in Tris-buffered saline with tween-20 (TBST, 20 mM Tris-base, 137 nM NaCl, 0.05 % Tween-20 (Merck), pH=7.6 ...
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bioRxiv - Immunology 2020Quote: ... All ELISAs were developed with 100 µl substrate solution per well containing 0.11 M sodium acetate (Merck), 0.1 mg/mL 3,5,3’,5’-tetramethylbenzidine (TMB ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were dehydrated in graded alcohol and further stained with 1% uranyl acetate (Merck, Darmstadt, Germany) in 70% alcohol ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... SEC separation was carried using a 0.300 mL/min flow rate of 50mM ammonium acetate (Merck Sigma) for 20 minutes in isocratic conditions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three triterpenoids (betulin [BE]) were extracted first from the media twice with 1.0 ml ethyl acetate (Merck) for 60 min in RT and centrifuged for 5 min in 15 000 rpm according to Cao et al ...
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bioRxiv - Cell Biology 2024Quote: ... pituitary suppression was achieved by administering a GnRH antagonist (0.25 mg of cetrorelix acetate, Cetrotide®; Merck Serono ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Genetics 2022Quote: ... 5 µg SA8 was dissolved in 10 µL 10 mM hydrochloric acid and digested with 20 ng/µL pepsin (Merck, cat. No. 10108057001) at 37 °C for 6 h ...
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bioRxiv - Neuroscience 2023Quote: ... before transcardially perfusing with 21-28 ml of ice-cold PBS and 4%PFA-0,12% picric acid (Merck, Søborg, Denmark). Spleens were excised and weighed ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 g/L peptone (Difco) and 20 g/L glucose (Merck Millipore). Synthetic complete medium without uracil (SC-URA) ...
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bioRxiv - Neuroscience 2020Quote: ... 20% water (Merck Millipore), and 50 pmol/ml of amino acid internal standard MIX AA (Cambridge) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% Tween 20 (Merck)) with subsequent storage at 4°C.
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bioRxiv - Genetics 2021Quote: ... and 20% ethanol (Merck). Once the colonies were stained ...
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bioRxiv - Synthetic Biology 2020Quote: ... 20 mM CaCl2 (Merck), pH 8 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% Tween-20 (Merck), 1% BSA (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% Tween-20 (Merck) 3 × 5 min before incubation with secondary antibodies (Table 3 ...
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bioRxiv - Cell Biology 2024Quote: ... and 20% ethanol (Merck). Once the colonies were stained ...
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bioRxiv - Cell Biology 2023Quote: ... and 20% ethanol (Merck). Once the colonies were stained ...
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bioRxiv - Cell Biology 2023Quote: ... PR619 (20 µM; Merck), CaCl2 (2 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.05% Tween-20 (Merck), 20% DMSO (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... 20 mM imdidazole (Merck) and 0.01% Tween-20 (Merck) ...
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bioRxiv - Neuroscience 2022Quote: ... then filtered using a 0.45μm spin filter (Spin-X centrifuge tube filter, 0.45 μm Cellulose Acetate, Merck, Germany) to remove particulates ...
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bioRxiv - Microbiology 2020Quote: ... Organic acids (lactate, acetate, pyruvate and succinate) were analyzed using a reversed-phased HPLC (Merck-Hitachi, Darmstadt, Germany) equipped with a diode array detector (L-7450 ...
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bioRxiv - Immunology 2022Quote: DNase activity was measured by incubating 0.25 μg/μL Lambda DNA with 0.5 M acetate/NaOH at pH 4.8 (Merck Millipore), 50 mM CaCl2(Merck Millipore) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All samples (fresh wight ~500mg, dry weight ~30mg) were extracted twice with 1.0 ml of ethyl acetate (Merck) by vortexing for 15 min and centrifuged for 10 min in 15000 rpm according to Cao et al ...
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bioRxiv - Microbiology 2022Quote: ... The ethyl acetate portion showing biosurfactant activity was concentrated and applied to silica gel column chromatography (Merck, Germany) eluted with CHCl3:MeOH (50:1 ...
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bioRxiv - Cell Biology 2024Quote: ... L-glutamine and antibiotic/antimycotics and decidualized with 1µM PGE2 or 1µM relaxin-2 (accession # P04090) (both Biotechne Ltd, Abbington, U.K.) in combination with 1µM medroxyprogesterone acetate (MPA) (Merck) for up to 4 days ...
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bioRxiv - Plant Biology 2021Quote: ... 14-3-3 binding was detected using anti-GST monoclonal antibody (Merck).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 0.15% 3-[(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Merck Chemicals Ltd.). NADH:decylubiquinone (DQ) ...