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Citations for Merck :
2851 - 2900 of 5053 citations for 8 chloro 10 3 dimethylamino propyl phenothiazin 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... with a SuperdexTM 200 Increase 10/300 GL (Cytiva) prior to up-concentration using Amicon® Ultra-0.5 Centrifugal Filter Units (Merck Millipore). The protein eluted as a dimer.
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bioRxiv - Microbiology 2022Quote: ... dialyzed in buffer C and then concentrated in a centrifugal concentrator with a 10-kDa molecular weight cutoff membrane (Merck Millipore).
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bioRxiv - Microbiology 2022Quote: ... cultures were maintained at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol ...
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bioRxiv - Immunology 2022Quote: ... Inhibition of respiratory burst was performed by adding 50 µg/mL of catalase to digest H2O2 and 10 µM of Diphenyleneiodonium chloride (DPI) to inhibit NADPH oxidase (Sigma-Aldrich, Merck) during the overnight coculture ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the preparation was washed three times with PBS for 10 min and mounted with FluorSave™ Reagent (Merck, Darmstadt, Germany). Confocal images were collected using the Leica TCS SP8 confocal microscope with HC PL APO CS2 20x/0.75 NA IMM ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... After incubation the mixtures were buffer exchanged in centrifugal filter units (Amicon Ultra, 0.5 ml, 10 K, Merck Millipore, Darmstadt, Germany) washed three times with 50 µl of sodium phosphate buffer (20 mM Na2HPO4 ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysate (30 μg) was loaded onto and run through a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck, IPVH00010). Membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2019Quote: ... Imidazole and salts were removed by three washing steps with 21 ml dialysis buffer (20 mM Tris-HCl pH 8.0, 200 mM KCl, 10 mM MgCl2) on Amicon 30 kDa filters (Merck Millipore, USA). Cas9 protein was diluted to 10 mg ml−1 in dialysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... peptides were loaded onto an in-house zwitterionic Zic-HILIC SPE cartridge containing 20 mg of Zic-HILIC particles (10 μm, 200 Å; Sequant/Merck). The flow-through was collected and re-circulated through the column an additional three times ...
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bioRxiv - Plant Biology 2021Quote: ... were moved to new tubes and concentrated at 14,000×g for 10 min at 4 °C using Amicon ultra-0.5 ml centrifugal filters (catalog number Z677094, Merck millipore, Sweden). Thylakoid ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated to 33 mg/ml using an Amicon Ultra 50 ml 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) and then aliquoted before flash freezing the protein sample in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... rhizoxinica were grown at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol.
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Cancer Biology 2020Quote: ... dipped in MilliQ, and mounted in Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Calbiochem, Merck Millipore, Darmstadt, Germany)) ...
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bioRxiv - Cell Biology 2020Quote: ... from affinity purification were pooled and buffer exchanged using Amicon™ Ultra-15 Centrifugal Filter Unit (10 kDa cutoff; Merck™) in 10 mM HEPES-NaOH buffer ...
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bioRxiv - Neuroscience 2021Quote: ... PBMCs were washed twice with PBS containing 1mM EDTA and subsequently frozen in 10% dimethyl sulphoxide (DMSO) (Merck KGaA, Hesse, Germany) and 90% foetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... 10 mL of CM or qEV fractions 6-10 diluted in PBS were loaded on 15 mL Amicon Tubes with a cut-off of 100 kDa (Merck, Germany) and spun for 1 hour RT at 5000 xg on a FA-45-6-30 fixed angle rotor ...
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bioRxiv - Microbiology 2020Quote: ... the apical medium was removed and basolateral medium was switched to differentiation medium ± 10 ng/ml IL-13 (IL012; Merck Millipore). Differentiation medium consisted of a 50:50 mixture of DMEM-H and LHC Basal (Thermo Fisher ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and concentrated using Amicon concentrator devices (molecular weight cut-off, 10 000 and for full-length protein 50 000; Merck Millipore). Recombinant His-tagged proteins (MA4376 ...
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bioRxiv - Microbiology 2020Quote: ... Purified DesX was desalted and concentrated by centrifugal filtration using an Amicon Ultra centrifugal filter unit (10 kDa cutoff; Merck Millipore). Before the assay ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of 10 µl of the reaction mixtures is spotted on HPTLC (High-Performance TLC) Silica Gel 60 Glass Plates (Merck KGaA) after centrifugation at 16.000 g for 2 min ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed by incubation with 2% Saponin in HBSS and lysates were serially diluted in 0.05% Tween-80 / dH20 and plated on 7H10 agar plates containing 10% heat-inactivated bovine serum (Merck, Darmstadt, Germany). Plates were incubated for 3 to 4 weeks at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... method [68] with the following modification: Equal volumes of spent media were concentrated to approximately 50 μL with Microcon - 10 kDa centrifugal filter units (Merck Millipore) at a speed of 14,000 x g ...
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bioRxiv - Immunology 2022Quote: ... Supernatant from doxycycline-induced or uninduced cells (40mL per condition) was collected and concentrated to 500 μL using Amicon Ultra-15 filters with 10 kDa cutoff (Merck Millipore). The concentrated samples were loaded on a Superose 6 10/300GL column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently visualized by Coomassie-stained SDS-PAGE before being concentrated using a 10 kDa MWCO Amicon Ultra centrifugal filter (Merck Millipore) to appropriate concentrations for further analysis.
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed 3x in TBST for 10 min again and afterwards incubated in Immobilon ECL Ultra Western HRP Substrate (Merck, WBULS0100) for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was loaded on a 5 mL Ni-Sepharose cartridge and the flow-through was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Cancer Biology 2022Quote: ... HIC1 KO cells were seeded on coverslips and DNA damage was induced by incubation with 10 μM etoposide (Sigma-Aldrich/Merck) for 1 hour ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Microbiology 2022Quote: ... This was followed by the removal of the buffer by placing the microtube in a magnetic rack and three times washing with IP buffer.200 μl IP buffer wasalong with fragmented RNA and 10 μl RNAse Inhibitor (Merck #CS216138) was then added to the antibody coupled to beads ...
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bioRxiv - Plant Biology 2022Quote: ... 2 plugs of C18 octadecyl 47mm Disks 2215 (Empore™) material and 1mg:10 μg of LiChroprep® RP-18 (Merck) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 10 min at room temperature and stained with the Hemacolor Rapid Staining Kit (Merck Millipore). Images were collected on BX61 upright microscope (Olympus ...
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bioRxiv - Plant Biology 2024Quote: ... the seeds of transformed plants were germinated and grown for 10 days on Murashige-Skoog (MS) plates containing Hygromycine B (Merck H3274). Resistant plants were transferred to soil and genotyped with the primers listed in Table S1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Neuroscience 2024Quote: ... Forebrain slices 250 µm thick were transferred in Millicell chambers and cultured for 4 hours in F12/DMEM medium supplemented with CFS 10% in a CO2 incubator (Merck Millipore) prior to pharmacological treatment and recording ...
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Cell Biology 2023Quote: ... except that SDS-PAGE was carried out using Millipore mPAGE® 10% Bis-Tris Precast Gels (MP10W10/ MP10W12/ MP10W15) with MES SDS running buffer (MPMES) from Merck, before protein transfer to PVDF membranes.
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bioRxiv - Immunology 2024Quote: ... All Hoxb8-Fl cell lines were cultured in very low endotoxin containing RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Biophysics 2024Quote: ... nsp16 and nsp7-11 were exchanged by six rounds of dilution and concentration in centrifugal filter units (Amicon Ultra, 10 K MWCO, Merck Millipore).
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated using an Amicon Ultra 10 kDa molecular weight cutoff centrifugal filter unit (Merck Millipore). The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...