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Citations for Merck :
2851 - 2900 of 4702 citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... neurons were stained with 1 μg/ml HOECHST-33342 (H-33342) and 1 μM calcein-AM (both from Merck, Darmstadt, Germany) one hour prior to the imaging ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Bioengineering 2022Quote: ... freeze-dried mucin-MA and unreacted mucin samples were separately dissolved at 1 mg mL−1 in 0.1 M sodium bicarbonate buffer pH 8.5 (Merck, Kenilworth, NJ, USA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated for 1 hour using the following antibodies: 1:500 rabbit anti-H3S10ph (Sigma-Aldrich, Merck, 06-570), 1:500 mouse anticyclin B1 (BD Pharmingen ...
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bioRxiv - Neuroscience 2024Quote: ... The different tau isoforms 3R and 4R were checked with monoclonal RD3 (1:200 dilution) (clone 8E6/C11) and RD4 (1:200 dilution) (clone 1E1/A6) antibodies (Merck Millipore). Other antibodies used were rabbit anti P62/ SQSTM1 (1:100 dilution ...
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bioRxiv - Biophysics 2023Quote: ... 5 µL of 25 µM GSK3β-phosphorylated tau fibrils were sonicated for 1 minute using a water bath sonicator (Bandelin Sonorex digitec) and then mixed with 1 µL of 1 mg/mL pronase (53702, Merck-Millipore). The mixture was then quickly added to the Quantifoil 2/1 grids and plunged-frozen after an incubation of 5 seconds using a Leica EM GP2 automatic plunge freezer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Sodium dodecyl sulfate (final concentration, 1 mg/mL) and proteinase K (final concentration, 1 mg/mL; MERCK Group Japan, Tokyo, Japan) were added to the sample ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906; Sigma-Aldrich, MERCK) for 1.5h and bands were visualized by chemiluminescence using the Clarity ECL Western blotting substrate (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... the membranes were washed thrice with TBS-T for 5 minutes each and then incubated with the respective secondary antibodies at room temperature for 1 hour - either 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Sigma-Aldrich/Merck, #A9169) or anti-mouse IgG (Sigma-Aldrich/Merck ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL) for 10 minutes, followed by base piranha cleaning (1:1:4 H2O2 (000855032300, Bio-Lab, IL):NH3 (105432, Merck, DE):H2O ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... PBS rehydrated tissue was then blocked using 10% normal horse serum in 0.2% Triton X-100 in PBS for ∼1 h and then primary antibodies specific for S100 (Merck, S2532, 1:200) TUJ1 (Synaptic systems ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Plant Biology 2021Quote: ... USA) was conditioned with 1 ml of 100% methanol and 1 ml of deionized water (Milli-Q, Merck Millipore, Burlington, MA, USA). After the conditioning steps ...
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bioRxiv - Biochemistry 2021Quote: ... The synthetic cassette also contained flanking NcoI and SalI sites that were used to clone the pre-array into MCS-1 of pCDFDuet™-1 (Novagen, Merck Millipore). Vibrio metoecus cas6f ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were derivatized for 1 hour at 85 °C using N-tert-Butyldimethylsilyl-N-methyl-trifluroacetamide with 1% tert-Butyldimethylchlorosilane (Merck, NJ, USA) and immediately analysed by GC-MS ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Microbiology 2024Quote: ... rivuli cultures were incubated with 1 mg of chitin suspended in 220 μL 1% PBS (phosphate buffered saline, Sigma Aldrich/Merck, US) in LC-MS grade water ...
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bioRxiv - Synthetic Biology 2023Quote: ... VpALDH and SlAlaR were combined (each at 0.05 μM) with 1 U mL−1 D-amino acid oxidase (DAAO) from porcine kidney (Merck product no. A5222) and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Fat pad tissue was minced and dissociated using the gentleMACS dissociator with an enzymatic digestion solution consisting of 1 mg ml−1 collagenase II (Merck Millipore, 234155), 1 mg ml−1 collagenase IV (Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was incubated overnight with primary antibodies (anti-PSD95, MA1-046, ThermoFischer Scientific, USA, 1:1000) and anti-stargazin Rabbit anti-stargazin (TARP γ2) (AB9876, Merck, 1:1000), washed with TBST ...
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bioRxiv - Immunology 2024Quote: ... The membrane was washed with PBST before adding the secondary HRP-coupled antibodies at 1:3000 dilution (α-rabbit, sigma) or at 1:3000 dilution (α-rat, Merck). Blots were visualised with ECL (Amersham ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in 50 µl of water and thoroughly mixed at 1:1 volumetric ratio with matrix solution (2,5-Dihydroxybenzoic acid) (Merck KGaA, Darmstadt, Germany). One µl of each sample was deposited onto the MALDI (Matrix Assisted Laser Desorption Ionization ...
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bioRxiv - Genetics 2024Quote: Cells were washed twice with PBS prior to protein extraction using a 1:1 mixture of PBS and 2x Concentrate Laemmli buffer (Merck Sigma-Aldrich) containing 1:1000 benzonase (Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: Dual-emission potential-sensitive fluorescence dye JC-1 (Merck) was used to measure mitochondrial membrane potential of cells following CAP treatment ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies for puromycin (1:25,000 dilution; MABE343, Merck), 1:1000 dilution of Esrra (ab16363 ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti–GAPDH (Mouse 1 μg/ml; Merck/Millipore). HRP-conjugated secondary antibodies were used as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... and adding 1 g of haematoxylin (Merck, cat. H9627). After complete dissolution ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Cux1 (1:100, ABE217; Merck Millipore). Nuclei were stained using Hoechst 33258 (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2022Quote: ... and probed with antibodies recognizing FAM21 (Merck, 1:1000), WASH (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 1:10000 mouse anti-tubulin (Merck Cat. No. T5168). Then ...
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bioRxiv - Neuroscience 2020Quote: ... TRA-1-60 (Merck Millipore, Germany; Cat. No. MAB4360), and TRA-1-81 (Merck Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-phospho Histone H3 (1/500, 06-570, Merck), and anti-ZO-1 (1/50 ...
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bioRxiv - Physiology 2021Quote: ... anti-Bcrp (1:100 – Bcrp [MAB4146]; Merck Millipore, USA), anti-Abcg1 (1:100 – [PA5-13462] ...
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bioRxiv - Biochemistry 2020Quote: ... GAPDH (MAB374, diluted 1:5000 for Western Blotting, Merck) and GFP (ab290 ...
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bioRxiv - Biochemistry 2020Quote: ... mix and 1 μL benzonase (250 units/μL, Merck) for 30 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... or actin (MAB1501R, 1:500; Merck-Millipore, Burlington, MA) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and rabbit anti-Tbr2 (1:1000, Merck Millipore, AB15894). Species-specific Cy2- ...
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bioRxiv - Neuroscience 2020Quote: ... 1× cOmplete ULTRA EDTA-free protease inhibitor cocktail (Merck)) ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and benzonase (2.5 ku, Merck Millipore) were added and cells were lysed using Emulsiflex (Avestin ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-rat IgG HRP(1:5000; Merck); and goat anti-mouse IgG HRP (1:5000 ...
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bioRxiv - Systems Biology 2021Quote: ... and 1% penicillin/streptomycin (p/s, Sigma-Aldrich/Merck). For differentiation ...
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bioRxiv - Plant Biology 2020Quote: ... autoclaved) with 1% β-Mercaptoethanol (Cat No.: M3148, Merck) and 100 μL of Ambion Plant RNA Isolation Aid (Cat No. ...