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Citations for Merck :
2801 - 2850 of 2911 citations for Mouse Anti Chikungunya Virus E2 Protein 18H01 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... blocked in 5% NGS and 0.5% Triton diluted in PBS and incubated over night at 4°C with primary antibody: rabbit anti-NG2 (1/50, AB5320, Merck), goat anti-PDGFRα (1/200 ...
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bioRxiv - Microbiology 2021Quote: ... 12,000g) and 500 µg of lysate was incubated with pre-washed EZview™ Red ANTI-HA Affinity Gel (E6779, Merck) or ANTI-Flag®M2 Affinity Gel (A2220 ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM Mif2-6xHis-6xFlag or Mif2 S217-240A-6xHis-6xFlag was incubated with 20 µM Mtw1-Nnf1-6xHis and immobilized on anti-FlagM2 beads (Merck) for 1 h at 4 °C and 1200 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK), HIF2A (Novusbio/NB100-122, Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK) and GAPDH (Merck/MAB374 ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... and the remaining supernatant was used for chromatin immunoprecipitation and mixed with magnetic beads (60 μl) coated with anti-FLAG M2 antibody (Merck). Immunoprecipitation was performed overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Double staining with Fluro Jade C and anti-SARS-COV2 Nucleocapsid antibody were performed by following the protocol from the Fluro Jade C supplier (AG325, Merck) with some minor modifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibody incubation was performed at room temperature for 90 min utilizing a horseradish-peroxidase (HRP)-labeled anti-rabbit IgG (Merck), and staining was visualized with DAB (Dako ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
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bioRxiv - Immunology 2021Quote: ... plates were fixed with 1:1 acetone and methanol Anti-S1 rabbit polyclonal antibody (Novus) was added to each well and incubated for 1Hr followed by incubation with FITC labelled anti-rabbit antibody (Merck). Fluorescence images were captured using an inverted microscope (ZeissAX10).
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bioRxiv - Molecular Biology 2020Quote: ... Another washing step was conducted as before and the Anti-Human IgE (ε-chain specific) detecting antibody (Merck, Darmstadt, Germany) was diluted 1:1000 (v/v ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... They were incubated 1h at RT in a blocking buffer (20% BR + 20% goat serum) and then overnight with an anti-DIG-AP antibody (1:2000, Merck) in the blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse Cy3 conjugated secondary antibody (cat no. AP124C) and anti-rabbit Cy3 conjugated secondary antibody (cat no. AP132C) were procured from Merck. Anti-rabbit Alexa Fluor 488-conjugated secondary antibody (cat ...
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bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... Fragmented RNA was subjected to two rounds of m6A immunoprecipitation for 2 hours each using an anti-m6A antibody (ABE572, Merck) previously conjugated to protein-A magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Molecular Biology 2022Quote: ... was assessed before and after induction with Doxycycline (1 μg/mL) by Western blot analysis using an anti-FANCJ (cat. B1310, Merck) and/or an anti-Flag antibody.
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bioRxiv - Neuroscience 2022Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... A sample of the supernatant was collected as “input” and the remaining solutions were either mixed or separately incubated with previously washed Anti-Flag M2 beads (Merck) by tumbling for 150 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... chromatin samples cross-linked by formalin were enzymatically shared and incubated with rabbit anti-AcH4 antibody or isotype control antibody (Merck) at 4 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated with an anti-sera peptide based polyclonal rabbit GLK2-antibody (1:3,000) and a peroxidase-conjugated anti-rabbit antibody (1:10,000) (Merck, Darmstadt, Germany) for 1 h at room temperature in TBST [Tris-HCl 20 mM (pH 7.6) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) in 1× PBS for 20 min at room temperature and then incubated with the anti-ADAR antibody HPA051519 (Merck) diluted 1:200 with PBS for 12 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin- labeled probes and biotin-labeled probes were detected with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/1,000 diluted streptavidin conjugated with DyLight 549 (Vector Laboratories ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody anti-Rabbit or/and goat IgG antibody conjugated with HRP (GENA934, AP106P, Merck) at room temperature for 1 hour for enhanced chemiluminescence ...
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bioRxiv - Cell Biology 2023Quote: ... the buffer was removed and 50 μl per well of monoclonal 3F10 anti-HA-Peroxidase (Merck Life Science, Gillingham, UK) 12.5 ng per ml in washing buffer was added and the plate incubated 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled fractions were diluted with 2 vols of KPEM buffer to achieve a final concentration of 125 mM KCl and then incubated for 1 hr with 4 mL anti-FLAG monoclonal antibody conjugated resin (M2 agarose, Merck) using rolling ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 µl of undiluted chromatin was incubated overnight at 4°C with Dynabeads M-270 Epoxy preincubated with anti-Tyr1P (MABE350, Merck), anti-Ser2P (C15200005-50 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were mounted using a mowiol-based mounting medium with DABCO (1,4-Diazabicyclo[2.2.2]octane) anti-fading agent (Merck Life Science SLU). Images were acquired using a Carl Zeiss-Axio Imager.M2 microscope and processed with ZEN 3.9 software (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... the localization was made visible by immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... Secondary incubation was completed with a 1:40 dilution of Goat anti-Rabbit IgG 10 nm gold conjugate for a further hour (Merck).
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bioRxiv - Immunology 2024Quote: ... we included anti-human PD-1 IgG4 S228P antibody (10μg/ml, 1B8/HuPD1B-3, Merck & Co., Inc., Rahway, NJ, USA). For TIM-3 blockade (αTIM-3) ...
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bioRxiv - Molecular Biology 2024Quote: ... the plates were incubated for 1 h at 25°C with peroxidase-conjugated donkey anti-chicken IgY (Merck, NJ, USA) diluted 5000 times with HEPES-EDTA containing 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an incubation overnight at 4 °C in blocking solution with anti-puromycin antibody (clone 12D10, MABE343; 1:500) AlexaFluor 488 or AlexaFluor 647 (MABE343-AF488 or MABE343-AF647; Merck). On the following day ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer of the anti-PTP1B antibody was exchanged to PBS-T using a Microcon-30kDa centrifugal filter unit (MRCF0R030, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were clarified by centrifugation and a minimum of 200μg of lysate was incubated with pre-washed EZview™ Red ANTI-FLAG® M2 Affinity Gel (F2426, Merck) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilised mitochondria were then clarified by centrifugation at 16,000 g for 10 min and incubated with pre-washed anti-Flag® M2 Affinity Gel (Merck; A2220) at 4 °C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies or lectin were used: an anti-aggrecan rabbit antibody (1:4500 for DAB staining; 1:500 for fluorescence staining; AB1031, Merck Millipore), an anti-GABA chicken antibody (1:200 ...