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Citations for Merck :
2801 - 2850 of 5854 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... AT2 Organoids were fixed with PFA for 3 minutes and then released from matrigel by incubating the culture with Corning Cell Recovery Solution (Merck, CLS354253) for 2 hours at 4 °C.
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM MgCl2) before addition of Laemmli buffer with 0.25 U/µL benzonase (final concentration, Merck Millipore) for 10 min followed by 5 min denaturation at 95°C.
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bioRxiv - Immunology 2021Quote: ... The plates were finally washed 5 times with HSWB before adding 100 µL of TMB (Merck, Germany) to each well ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Table S1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used a slightly modified version of the iron-limited CAA medium: 5 g Casein Hydrolysate (Merck), 0.25 g MgSO4*7H2O (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and purified by filtration through 5 μm pore polycarbonate membranes (Merck Millipore Co. Cork, Ireland).
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bioRxiv - Cancer Biology 2022Quote: PEGylated-poly T coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 minutes followed by wash with IMB ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBS and blocked with 5% normal donkey serum (NDS) (Merck-Millipore), 0.1% Triton X-100 in PBS for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with 2.5 µM IWP4 (Stemgent, Cambridge, MA, USA) and 5 µM XAV939 (Merck, Kenilworth, NJ, USA). The culture medium was refreshed with RPMI□+□B27+insulin medium every other day ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared via centrifugation and loaded on two 5 mL HiTrap Heparin HP (Cytiva, Merck) columns (connected in series ...
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bioRxiv - Neuroscience 2022Quote: Conditioned media from astrocytes were collected at 5 DIV and filtrated with Millex-GP filter (SLGPR33RS, Merck). ACM was concentrated with Amicon Ultra 10kDa (UFC501008 ...
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bioRxiv - Cell Biology 2022Quote: ... at a final concentration of 5 μg/ml in M2 medium or Latrunculin B (428020-1MG, Merck) at a final concentration of 5 μM in M2 medium (to disrupt F-actin) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Supplemental Table 2 ...
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bioRxiv - Immunology 2020Quote: ... Lysates were further cleared by sterile filtration employing a 5 µm filter unit (Merck Millipore, Darmstadt, Germany). The first column contained 1 mg of W6/32 antibody coupled to sepharose ...
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bioRxiv - Systems Biology 2019Quote: ... a SeQuant ZIC-pHILIC (100 mm, 2.1 mm I.D. and 5 μm particle size; Merck, Damstadt, Germany) column was used ...
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bioRxiv - Immunology 2021Quote: ... pH 5 (0.1 M citric acid monohydrate from Sigma and 0.2 M disodium phosphate dihydrate from Merck)) was added to the plate and incubated for 30 minutes in the dark ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml Hemin (Mercury Cat#3741) and 5ul/ml vitamin K1 (Merck Cas#84-80-0)) under anaerobic conditions.
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 5 times with PBS-T and 100µl of 3,3′,5,5′-Tetramethylbenzidine (TMB) (Merck Millipore) equilibrated at room temperature were added ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were then incubated with 10 ml blocking buffer (5% skim milk powder (Merck Millipore, 115363) in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Metabolite separation was achieved by using a ZIC-pHILC column (150 x 4.6 mm, 5 μm; Merck) and Dionex UltiMate 3000 liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... At L4 larval stage animals were transferred to plates containing 10 µM of 5-Fluorouracil (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1.2 mL of a 10 mM sodium periodate solution (Merck, cat# 7790-28-5) was added to the suspension ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then cultured in G-IVF PLUS (Vitrolife, #10136) under mineral oil (Merck, # 8042-47-5) at 37°C in 5% O2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... twice in 100% ethanol for 5’ each followed by washing in Xylene (Sigma-Aldrich, Merck, Overijse, Belgium) twice for 5’ each ...
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bioRxiv - Biophysics 2023Quote: ... the actin was mixed with a 5-fold molar excess of NHS-XX-Biotin (Merck KGaA, Germany) and incubated in the dark for 15 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The heptose-phosphates were separated by a SeQuant ZIC-pHILIC (5 µm, 150×2.1 mm) column (Merck). Solvent A ...
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bioRxiv - Genetics 2023Quote: ... At L4 larval stage animals were transferred to plates containing 10 µM of 5-Fluorouracil (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Microbiology 2023Quote: ... 5% H2) at 37°C in Bacteroides minimal medium (BMM) containing 27.5 μM of iron (FeSO4, Merck)91 ...
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bioRxiv - Cancer Biology 2023Quote: PEGylated-poly(T)-coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were treated with 0.5% Periodic acid for 5 min and stained in Schiff’s reagent (Merck) for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... 250-mm long) using an acetonitrile gradient (5–35% acetonitrile for 160 min, Merck KGaA, Darmstadt, Germany) in the presence of 0.1% formic acid at a flow rate of 250 nl/min ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with PBS and counterstained with a Hoechst-34580 DNA dye (5 μg/mL; Merck) in some cases supplemented with combinations of UEA1 Atto 488-conjugated lectin (10 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... and treated for 24h with 10 μM Nutlin 3a and/or 5 μg/ml 17β-Estradiol (Merck). Four independent experiments were performed ...
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bioRxiv - Biophysics 2024Quote: ... mN2GCH was concentrated down to 500 μl using an Amicon Ultra 5 centrifugal filters (Merck, Darmdstad, Germany) and then injected onto a Superdex 200 10/300 GL column (Sigma-Aldritch ...
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bioRxiv - Molecular Biology 2024Quote: ... equal amounts of 100 μM 5’-Cy5 labelled forward strand and unlabelled reverse strand oligonucleotides (Evrogen/Merck) were mixed in the annealing buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... after which they were washed three times in PBST and blocked overnight at 4°C in 10% serum (Merck) in PBS/0.3% TritonX100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449; Merck Millipore, Burlington, MA, USA) or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745 ...
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bioRxiv - Microbiology 2020Quote: ... RNA pellet thus obtained by centrifugation at 16,000 × g for 20 min (4°C) was washed using 70% ethanol (Merck) and resuspended in nuclease-free water after air drying ...
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bioRxiv - Physiology 2020Quote: ... Proteins in the conditioned medium were concentrated up to 1.2ug/ul of total protein using cellulose membrane Ultra-4 Centrifugal Filter Unit of 3KDa cut-off (Merck) as per the manufacturer instructions.
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with a total of 300 ng DNA (per well of a 4-well 3.5 cm dish) using 0.75 µl Genejuice (Merck Millipore) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2019Quote: ... Approximately 4×105 of the cultured cells were then transferred to each well of 96-well filter (Merck Milipore) pre-coated with 2 µg/ml TT in 1x PBS or only 1xPBS ...
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bioRxiv - Biophysics 2020Quote: ... fluorescently-labeled and biotinylated H57-scFV were again concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK) and stored in PBS supplemented with 50% glycerol at −20°C ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed 2x 4 min in PBS and subsequently overlaid with 0.1 mg/ml collagen IV (C7521, Merck) and placed at 4°C overnight (ON) ...
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bioRxiv - Cancer Biology 2021Quote: Cells grown on coverslips were washed with PBS and incubated 15 min in 4% formaldehyde (formalin solution buffered at pH 6.8, Merck). Cells were washed once with 50 mM ammonium chloride (Carl Roth ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... and then equal volumes were concentrated 50-fold using Amicon Ultra-4 centrifugal filter units (Merck Millipore, Watford, U.K.). For analysis of cell-associated proteins ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... previously equilibrated with 50 mM HEPES buffer (pH 7.0) and concentrated with an Amicon Ultra-4 10K centrifugal filter (Merck).