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Citations for Merck :
2701 - 2750 of 3327 citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... anti-A17L (1:500) in combination with horseradish peroxidase (HRP)-coupled secondary antibodies (1:2,000) and Immoblion Forte Western HRP substrate (Merck) or with IR-Dye secondary antibodies (Licor ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
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bioRxiv - Immunology 2021Quote: ... Samples were postfixed with 0.1 M sodium cacodylate buffer supplemented with 1 % osmium tetroxide (Science Services) and potassium ferrocyanide (Merck) and embedded in 1 % agarose (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies diluted in 5% milk/PBS-Tween were: mouse anti-Actin (1:2000; Merck, Germany, Cat. No. MAB1501), rat anti-MYC (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: Type 1 diabetes was induced with a single intraperitoneal (i.p.) injection of 150 mg/kg streptozotocin (STZ; Merck, France) freshly dissolved in 0,9 % NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were then dried and stained on a heating block with a 1% toluidine blue (Merck, 89640-5G) solution including 2% Borax for 90 s at 60°C ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Bioengineering 2020Quote: ... labeled for 30 minutes at 4ºC and washed with PBA (PBS with 0.2% sodium azide [Merck]; 1% BSA [Sigma]).DAPI (final concentration of 1 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Pathology 2021Quote: ... 96-well plate was coated with 1 μg/ml monoclonal anti-lubricin antibody clone 9G3 (Merck Millipore, Billerica, MA) in TBS at 4°C over-night ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-actin monoclonal antibody (#MAB1501R, used at 1:5,000 dilution for the Western blot analyses, Merck Inc.); a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374 ...
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bioRxiv - Cell Biology 2021Quote: ... Surfaces were functionalised by flooding the device with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500™ (3M™) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged at 70 – 90% confluency using 1% PBS and trypsin/EDTA (0.25%,0.02%) in T75 tissue culture flasks (Merck, Australia) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... pellets were resuspended in urea lysis buffer (8 M Urea, 50 mM Tris pH 7.5, and 1:200 freshly added protease inhibitor cocktail (Merck)) ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... cells were starved overnight in serum-free DMEM and then treated for 1 hour with 5 ng/ml TGFβ1 (Preprotech) or with 5 μM SB431542 (Merck), and intensities quantified by ImageJ ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Microbiology 2020Quote: ... NO2- concentrations were determined using colorimetric test strips (NO2--test, 0-10 mgNO2-N·L-1, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Microbiology 2022Quote: Nasal sIgA was obtained from 1 ml nasal swab eluates by centrifugation (1 minute, 10 000 xg) and supernatant was sterile-filtered through a 0.4 μm pore (Merck). We determined sIgA concentrations by ELISA (Human IgA ELISA kit ...
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... The pH of the solution was adjusted to pH 7.1-7.4 with 1 M NaOH (Merck KGaA, Darmstadt, Germany) and filtered with a silicone- and latex free syringe (#4050-X00V0 ...
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bioRxiv - Neuroscience 2022Quote: Fixed tissue was cut into 1 cm fragments and first impregnated during 3h in a 15%-sucrose solution (Merck) in 1X PBS ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted 1:400 v/v in 0.5% low gelling temperature agarose in Ames’ medium at 37°C (A1420; Merck). A thin film was polymerized over a pure agarose meniscus in a Petri dish and covered with Ames’ medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The Lipid II extract was assessed by spotting 1-2 μL on a HPTLC silica gel 60F254 plate (Merck). The TLC plate was developed in a mixture of chloroform:methanol:water:ammonia (88:48:10:1 ...
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bioRxiv - Neuroscience 2022Quote: ... the second step consisted of incubating overnight in PBS-Triton with rabbit anti-Aldh1a1 (1:500, HPA002123, Sigma Merck) and goat anti-calbindin (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and lysed in RIPA buffer (50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 % (v/v) Triton X-100 (Merck), 0.5 % (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... the CDS of wild type (WT) PpGS1b.1 and PpGS1b.2 were subcloned into pET30a vector (Merck, Darmstadt, Germany) including a N-terminal 6xHis-tag by PCR ...
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bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred on PVDF membranes and rabbit polyclonal antibodies were used at a 1:500 dilution (anti-Pic and anti-SepA) or a 1:250 dilution (anti-SigA) in 1X TBS supplemented with 0,5% of Tween 20 (Merck) and 3% BSA (Euromedex) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... tyrosine-hydroxylase (TH) was detected with the primary antibody anti-TH in rabbit (1:600, EMD Millipore - Merck, USA). After washing in PBS ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated o/n at 4°C in 10 ml washing buffer (1% [w/v] milk powder in TBS-T) containing a 1:10,000 dilution of anti-pan-ADP-ribose binding reagent MABE1016 (Merck) at 4°C48 ...
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bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
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bioRxiv - Bioengineering 2024Quote: ... the THP-1 macrophages were detached from the MadSurface by incubating in 1mL/well Accutase (Merck Life Science NV) in the incubator for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... The cytoskeleton was stained using a 1:40 dilution of Alexa Fluor 488 conjugated phalloidin (Merck Life Science NV) in PBS and nuclei were stained using a 1:1000 dilution of DAPI (Merck Life Science NV) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 4 times for 5 min with 1 x PBS/Tween20 0.1 % (v/v) and the HRP conjugated secondary antibodies (A5420 and A4416, Merck) were added 1:5000 (goat ...
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bioRxiv - Neuroscience 2023Quote: ... For the example images for survival assay neurons were incubated with chicken polyclonal anti-MAP2 (1:2000; Merck Milipore) and secondary antibody Alexa Fluor 488 ...