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Citations for Merck :
2651 - 2700 of 2799 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Then sections were blocked for 45 min using a blocking buffer containing 5% goat or donkey serum (depending on the host species of the secondary antibody) (#G9023 and #D9663, Merck) and 0.3M glycine (#56-40-6 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: Proximity ligation assays with Aurora A kinase and MP-GAP were performed in HeLa cells using mouse anti-Aurora A Kinase antibody (1:500, A1231 Merck), rabbit anti-MP-GAP antibody (1:250 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) in 1× PBS for 20 min at room temperature and then incubated with the anti-ADAR antibody HPA051519 (Merck) diluted 1:200 with PBS for 12 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were incubated for 1 h at RT with a mouse anti-2A primary antibody (cat. no. MABS2005, Merck) diluted 1:2000 in 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... the fixation procedure above with and without subsequent membrane permeabilization was used in infected/uninfected organoids that were then stained with a mouse monoclonal anti-mitochondria antibody Cy3 conjugate (Merck) incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled fractions were diluted with 2 vols of KPEM buffer to achieve a final concentration of 125 mM KCl and then incubated for 1 hr with 4 mL anti-FLAG monoclonal antibody conjugated resin (M2 agarose, Merck) using rolling ...
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibody was washed again three times with PBS and the cell nuclei were then counter stained with DAPI (Merck). Epifluorescence microscopy was done in an inverted Olympus IX70 and Confocal microscopy was done in a Zeiss Axioscope 2 Plus microscope equipped with a Bio-Rad Radiance 2100 laser scanning system and Lasersharp 2000 imaging software and a Leica SP8 inverted focus microscope and analyzed with the Leica Application Suite (Las ...
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bioRxiv - Microbiology 2023Quote: ... and a 1:500 dilution of an antibody against the phosphorylated form of H2A histone family member X (γH2Ax) (clone JBW301, Merck) and 1:200 phalloidin coupled to Alexa Fluor 568 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight with an anti-GFP rabbit antibody (0.5 μg/ml) (Tamamaki et al., 2000) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000; MAB3120, Merck Millipore). After a rinse ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... the Immunoprecipitation (IP) was performed following a standard IP protocol and using the following antibodies: H3 (Reference: 07-690, Merck), H3K9me2 (Reference ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... 1:10,000 WB) and mouse monoclonal antibody recognizing the N-terminus β-Actin (#A5441, 1:10,000 WB) were purchased from Merck. Rabbit polyclonal phosphomyosin (Thr18/Ser19 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7, sc-13130, lot no # B1422) or control mouse IgG (12-371, Merck) in IP Buffer with 2X SDS buffer (100mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... fluorescent visualization of the nonisotopic signals used anti-digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1/200; Merck). This was followed by the deposition of biotin tyramide (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the localization was made visible by immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... in Tris- buffered salione containing 0.1% Tween-20 (v/v) (TBST) fore 60 mins at RT and incubated overnight with primary antibody against REST (1:500) (07-579, Merck), H3K9me2 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87 ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 5-10 μg of primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) in 250 μL LB3 with 1% Triton X-100 overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an incubation overnight at 4 °C in blocking solution with anti-puromycin antibody (clone 12D10, MABE343; 1:500) AlexaFluor 488 or AlexaFluor 647 (MABE343-AF488 or MABE343-AF647; Merck). On the following day ...
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bioRxiv - Immunology 2024Quote: ... we included anti-human PD-1 IgG4 S228P antibody (10μg/ml, 1B8/HuPD1B-3, Merck & Co., Inc., Rahway, NJ, USA). For TIM-3 blockade (αTIM-3) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer of the anti-PTP1B antibody was exchanged to PBS-T using a Microcon-30kDa centrifugal filter unit (MRCF0R030, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... bound secondary antibodies were detected using either EZ-Chemiluminescence Reagent (Geneflow Ltd) or Luminata™ Forte Western HRP Substrate (Merck Millipore) and a Biospectrum 500 imaging system (UVP ...
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bioRxiv - Developmental Biology 2021Quote: ... the following antibodies were treated to the organoids and the tissue slices at 4°C overnight: proSFTPC (1:200; Merck, AB3786), E-cadherin (1 ...
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bioRxiv - Genomics 2019Quote: ... We sequenced two biological and two technical replicates per isolate and used antibodies against the euchromatin histone mark H3K4me2 (#07–030, Merck Millipore). Sequencing was performed at the OSU Center for Genome Research and Biocomputing (Oregon State University ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...
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bioRxiv - Immunology 2021Quote: ... Histone neutralisation experiments were performed via intraperitoneal injection with dialysed and combined a-Histone 3 and a-Histone 4 antibodies (Merck Millipore) or control polyclonal rabbit IgG (BioXCell) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunofluorescence using primary polyclonal antibodies that label neurons (NeuN, mouse; 1: 1000, Millipore MAB377, Merck Millipore), reactive astrocytes (GFAP ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemistry was performed on colon sections as described earlier on brain sections58 using additional antibodies (α-Syn: ab27766, Abcam or L001, Enzo; NeuN: MAB377, Merck; MAP2 ...
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bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies or lectin were used: an anti-aggrecan rabbit antibody (1:4500 for DAB staining; 1:500 for fluorescence staining; AB1031, Merck Millipore), an anti-GABA chicken antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed and stained according to the procedure described above using primary antibodies against NESTIN (Merck, ABD69, 1:300 dilution) and TUBB3 (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Microbiology 2020Quote: ... Fifty picomolar DNA were incubated with 50pM anti-digoxigenin-covered beads (antibody: Roche, #11093274910 and carboxylate-modified beads F1-XC030, Merck-Estapor) in Buffer A (PBS 1X ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... and detected using primary and corresponding HRP-conjugated secondary antibodies on Fusion SL imaging system (Vibler) using Immobilon Western Chemiluminescent HRP Substrate (Merck, WBKLS0500). Molecular size of bands is marked in each panel [kDa] ...
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bioRxiv - Molecular Biology 2022Quote: Antibody-bead mixture was washed twice in iCLIP lysis buffer to remove unbound antibody and RNase-treated cell lysates were added alongside cOmplete EDTA-free Protease Inhibitor Cocktail (Merck, #11836170001) and incubated for 2 hours at 4°C on a rotating wheel ...
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bioRxiv - Biophysics 2022Quote: ... for 10 min prior to fixation after washing out puromycin were fixed with 4% PAF for 30 min at 37°C and subjected to immunoblotting using puromycin antibody (Merck, MABE343). For the negative control ...
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bioRxiv - Immunology 2022Quote: ... Antibody preparations for in vivo infusions were micro-filtered (Ultrafree®-CL devices - 0.1 µm PVDF membrane, Merck-Millipore, Darmstadt, Germany), and checked for endotoxins levels using the ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript).
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore ...