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Citations for Merck :
2651 - 2700 of 2965 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by washing with 1XPBS two times for 2’ each and blocked again with Biotin (0.001%, Sigma-Aldrich, Merck, Overijse, Belgium) for 20’ and washed twice for 2’ in 1XPBS each ...
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bioRxiv - Paleontology 2023Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... ParT (S48C)-His6 and ParT (Q271C)-His6 aliquots were supplemented with 1 mM tris (2-carboxyethyl) phosphine hydrochloride (Merck; cat# C4706) before flash-freezing in liquid nitrogen.
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bioRxiv - Biophysics 2019Quote: ... Constructs of Kmpv1 and KmpvSP1 were transiently expressed as fusion proteins with GFP on the C-terminus using the liposomal transfection reagent GeneJuice® (MERCK KGaA, Darmstadt, Germany). Measurements were performed at room temperature in a bath solution containing ...
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bioRxiv - Bioengineering 2019Quote: ... 40 µg of total protein were run on a 12% SDS-PAGE and transferred onto a 0.45 µm Immobilon®-P Transfer Membrane (Merck Millipore, Cat. NO#IPVH00010). The membrane was hybridized with a custom antibody at a 1 µg/mL dilution (GenScript ...
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS- PAGE and those containing pure tag free CBX8 protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer consisting of 20mM MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated to the desired concentration using centrifugal filters (#UFC800308; Amicon® Ultra centrifugal filters, Ultracel® −3K or larger; Merck Millipore Ltd.)
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bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
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bioRxiv - Cell Biology 2020Quote: ... ChIP assays were performed using a specific kit (Merck Millipore, Darmstadt, Germany). After crosslinking ...
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bioRxiv - Cell Biology 2020Quote: PP2A activity were measured by PP2A phosphatase activity assay kit from Merck Millipore (PP2A immunoprecipitation phosphatase assay kit ...
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bioRxiv - Microbiology 2021Quote: ... the serum levels of total GLP-1 (ELISA kit, Merck, Darmstadt, Germany) and IAA (ELISA kit ...
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bioRxiv - Microbiology 2021Quote: ... and a solution of XTT/PMS (Cell Proliferation Kit II; Merck, Germany) and culture medium was added to the wells ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using a Magna Nuclear RIP Kit from Merck Millipore (17-10520 ...
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bioRxiv - Cell Biology 2022Quote: We performed the proximity ligation assay129 using the Duolink PLA Kit (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was purified using the GenElute Mammalian total RNA Miniprep kit (MERCK). Samples from cell culture were harvested in the RNA extraction buffer supplemented with 10mM ß-ME ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cell viability was studied using Cell Counting Kit-8 (CCK8, Merck, Germany). VeroE6 cells were seeded at 10,000 cells per well of flat bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The resulting RNA was then subjected to amplification (Merck WTA2 Amplification Kit) to produce cDNA that was subsequently purified by column capture (Qiagen QiaQuick PCR purification kit ...
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bioRxiv - Immunology 2021Quote: QCM Boyden chamber chemotaxis neutrophil migration assay kits were purchased from Merck Millipore (ECM505 ...
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bioRxiv - Microbiology 2021Quote: ... KOD Xtreme Hotstart DNA polymerase kit was obtained from Merck (Darmstadt, Germany). T4 DNA ligase was obtained from New England Biolabs (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ELISA kits were used for measurements Growth Hormone (Merck-SIGMA EZRMGH-45K) according to manufacturers’ instructions.
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bioRxiv - Immunology 2022Quote: ... or with vaccine RNAs using RiboJuice™ mRNA Transfection Kit (Merck Millipore) according to the manufacturer’s instructions and incubated for 18 h ...
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bioRxiv - Plant Biology 2024Quote: ChIPCqPCR assays were performed using a commercial chromatin immunoCprecipitation Kit (Merck Millipore) in accordance with the method described by Hu et al ...
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bioRxiv - Developmental Biology 2024Quote: Proximity ligation was performed using Duolink PLA assay kit (Merck, Cat. #DUO92102) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Merck, DUO92101-1KT) was used to detect protein-protein interactions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... QPCRs were performed using a KAPA SYBR FAST kit (Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... We measured Leptin levels using mouse leptin ELISA kit (Merck Life Sciences) according to manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... plasmid DNA was extracted (GenElute HP Plasmid Midiprep Kit, Merck #NA0200-1KT) from all of the E ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma leptin and insulin levels were measured by murine ELISA kits (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... stained with haematoxylin and eosin (H&E) using a kit (Merck, Germany) and myotube diameter measured by light microscopy analysis of DIC captured images (Olympus CKX41 ...
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bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were allowed to adhere overnight (16 h) in the presence of 2 mM Thymidine (S-phase block; Merck, Darmstadt, Germany) and released into fresh medium ...
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bioRxiv - Neuroscience 2021Quote: TEER across cellular monolayers was measured with chopstick electrodes in 24-well Transwell inserts using the Millicell® ERS-2 Voltohmmeter (Merck Millipore). The absolute TEER of eGFP-hCldn5-MDCK II cells before treatment was recorded as a baseline reading ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Semithin sections (1–2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac ...
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bioRxiv - Biochemistry 2021Quote: ... After adding 1 drop of bovine thrombin (Biomed, Lublin, Poland) to 2 drops of fibrinogen (1mg/ml; Sigma-Aldrich, St. Louis, MO; Merck KGaA) the cells were entrapped within the fibrin clots ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequent thorough rinsing with ultrapure water (18.2 MΩ at 25 °C, maximum 2 ppb total organic carbon, Milli-Q Integral 5 Water Purification System, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biochemistry 2020Quote: DNA encoding for Pry1 and Na-ASP-2 were PCR amplified and cloned into NcoI and XhoI restriction sites of pET22b vector (Novagen, Merck, Darmstadt, Germany), which contains a pelB signal sequence to direct the secretion of expressed protein into the periplasmic space ...