Labshake search
Citations for Merck :
2501 - 2550 of 2746 citations for 6 chloro 4 methyl 2 phenylimino benzo b thiophen 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... The Lipid II extract was assessed by spotting 1-2 μL on a HPTLC silica gel 60F254 plate (Merck). The TLC plate was developed in a mixture of chloroform:methanol:water:ammonia (88:48:10:1 ...
-
bioRxiv - Plant Biology 2022Quote: ... the CDS of wild type (WT) PpGS1b.1 and PpGS1b.2 were subcloned into pET30a vector (Merck, Darmstadt, Germany) including a N-terminal 6xHis-tag by PCR ...
-
bioRxiv - Molecular Biology 2022Quote: ... samples were cleaned with a solid phase extraction method using 2 layers of C18 Empore™ SPE Disks (Merck) followed by 2 layers of SCX strong cation exchange Empore™ SPE Disks (Merck) ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated in xylene for at least 2 min and then covered with mounting solution Entellan ® (Merck). Images were acquired at a S360 Hamamatsu slide scanner using a 40x objective ...
-
bioRxiv - Neuroscience 2024Quote: ... concentrated using 100-kDa molecular weight cut-off (MWCO) filters Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at - 80°C ...
-
bioRxiv - Plant Biology 2024Quote: A pTYB2-based vector for the expression of PSM polypeptides and a pCDF-based vector that contained heme oxygenase 1 and phytochromobilin synthase genes for the synthesis of PΦB (Muramoto et al., 1999; Kohchi et al., 2001) were transformed into the Escherichia coli Rosetta 2 strain (Merck). E ...
-
bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected as control and the beads were thoroughly washed 3 x with TBS/Tween 0.2% after which bound proteins were eluted by adding hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck) and boiling for 5 min at 99 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... medium—composed of 6.7 g Yeast Nitrogen Base without amino acids (Difco 291940) and 0.8 g complete supplement mixture drop-out (Formedium DCS0019) in 1 L—supplemented with 2% glucose (Merck). Cells from glycerol stocks or agar plates were inoculated into YPD and incubated at 30°C on an orbital shaker at 250 rpm (in an Innova 4230 incubator ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...
-
bioRxiv - Biophysics 2023Quote: ... in digestion buffer (800 mM guanidine HCl, 2 mM CaCl2, 0.5 % Triton X-100, in 50 mM TRIS, #8382J008706, Merck), overnight at 50°C ...
-
bioRxiv - Cell Biology 2023Quote: ... To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml, Merck, Cat.N. 3072) for the duration of the experiment ...
-
bioRxiv - Immunology 2023Quote: ... Afterward, the cells were washed twice in R10 media (RPMI-1640 with 10% FBS, 2 mM glutamine (G7513, Merck), 1% penicillin-streptomycin (P0781 ...
-
bioRxiv - Genomics 2023Quote: ... a stock solution of 2 mg/mL colistin sulfate (Sigma-Aldrich Merck KGaA, Germany; 100 mg, 15,000 U/mg) was prepared and aliquots were stored at −80 °C ...
-
bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were washed trice for 10 min with 0.05% Tergitol in TBS (Tris-Buffered Saline) and blocked for 1 hour with 2% BSA (Merck) in TBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... They were further incubated with a 2× volume of 0.25× diluted TES buffer with a trace amount of benzonase nuclease (Merck) at 4°C for 45 min.
-
bioRxiv - Cell Biology 2023Quote: ... specimens were post-fixed for 2 h at RT in 1% osmium tetroxide in 0.05 M cacodylate buffer pH 7.4 (Merck, Germany) and dehydrated stepwise in a graded ethanol series followed by 100% acetone ...
-
bioRxiv - Neuroscience 2023Quote: ... they were washed 3 times with 1X PBS for 5 minutes and incubated with DAPI (4’6-Diamidino-2-phenylindole; 1 µg/mL, MERCK) for 5 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections or cells were pre-incubated in blocking buffer containing 2–10% normal donkey serum (Merck Millipore, Burlington, MA) and 0.1% Triton X-100 at RT for 1 h and incubated overnight at 4 °C with primary antibodies and subsequently incubated with secondary antibodies conjugated with Alexa Fluor 488 ...
-
bioRxiv - Microbiology 2024Quote: ... coli SM10 pCVD442-up-dotB-down and the SP-1303 ΔdotB mutant by mixing equivalent volumes of adjusted overnight (O/N) liquid culture to OD=2 on a 0.45 µm filter (peel corner, MERCK) positioned on a LB agar plate for 6 h at 28°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
-
bioRxiv - Molecular Biology 2024Quote: ... On-beads trypsin digestion was then performed by resuspending the beads in 200 µL of Digestion Buffer supplemented with 2 µL of 0.5 µg/µL Sequencing Grade Trypsin (Merck). Samples were incubated overnight at 37°C with constant agitation at 750 rpm ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 washes with 1X HBSS and an incubation in a trypsin-based digestion solution containing DNAse I (Merck, D5025) for 15 minutes at room temperature (RT) ...
-
bioRxiv - Neuroscience 2024Quote: ... whose composition is as plating media but with 5% FBS and 2 μM cytosine β-d-arabinofuranoside (Merck, C6645) to limit glial growth ...
-
bioRxiv - Plant Biology 2024Quote: ... 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 2% w/v PVPP, 10 mM DTT, 0.2% IGEPAL® [Merck]) supplemented with EDTA-free protease inhibitor tablets (Roche) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
-
bioRxiv - Genomics 2021Quote: ... The lysate was incubated for 2 hours at 4°C with 180 µl of anti-flag agarose beads (50 % slurry, ANTI-FLAG® M2 Affinity Gel, Merck, Kenilworth, NJ, USA), prewashed 3 × with 1 ml isotonic wash buffer (IsoWB ...
-
bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: HPLC separations were performed on a Waters 2695 separations module equipped with a Waters 2996 photodiode array detector and HPLC analysis was carried out using a LiChrospher® 100 RP-18 column (4 mm × 250 mm, 5 μm) (Merck, Kenilworth, NJ, USA). The mobile phase consisted of two solvent reservoirs ...
-
bioRxiv - Biochemistry 2023Quote: ... we first added 5 μL of clean PBS buffer to a silicone gasket (Grace Bio-Labs reusable CultureWell™ gaskets, 50 wells, 3 mm x1mm, Merck Life Science UK Limited) mounted on the clean coverslip to find the glass/buffer interface ...
-
bioRxiv - Molecular Biology 2019Quote: ... the remaining ultracentrifuged supernatant was concentrated to approximately 220 µL using Amicon Ultra-2 10 kDa molecular weight cutoff filters (Merck KGaA ...
-
bioRxiv - Physiology 2022Quote: ... frozen fresh tissue (∼20-45 mg) was placed into a 2 ml sterile microcentrifuge tube pre-loaded with ∼15-20 glass beads (Merck) while 200 μL of ice-cold methanol (Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated overnight with a primary antibody mixture of guinea pig anti-glycine transporter 2 (GlyT2; 1:10,000; AB1773, Merck, RRID:AB_90953) and mouse anti-GAD67 (1 µg/ml ...
-
bioRxiv - Molecular Biology 2019Quote: ... The proteins were again diluted in 9 volumes of Exchange Buffer 2 (Exchange Buffer 1 with 200 mM NaCl) and concentrated with 30 MWCO Amicon Ultra-15 (Merck).
-
bioRxiv - Neuroscience 2019Quote: ... Samples were digested by addition of porcine trypsin (enzyme to protein ratio of 1:100; for 2 x104 microglial samples, 300 ng was used; Merck) and incubated overnight at 37 °C with shaking ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 ml of pre-warmed 2xSSC with or without RNAseA (50 μg/ml final concentration, Merck) and incubated 1h30 min at 37°C ...
-
bioRxiv - Bioengineering 2021Quote: ... The cells were transduced with LV particles at low multiplicity of infection (MOI: 1-2) in the presence of 10 µg/mL of polybrene (Merck). HEK293T-EGFP were selected with 1 µg/mL of puromycin ...
-
bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
-
bioRxiv - Biochemistry 2020Quote: ... were incubated in standard assay buffer at 37°C and 2 µL aliquots were removed at appropriate time points and spotted on a silica gel 60 F454 plate (Merck). The separation was carried out in butanol:ethanol:milliQ water (5:3:2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Mouse LL/2 (LLC1; ATCC no. CRL-1642; obtained in 2015) was grown in BME with Earle′s salts (Merck) with the same supplementation as mentioned above ...
-
bioRxiv - Cell Biology 2021Quote: Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad) ...
-
bioRxiv - Plant Biology 2021Quote: ... (+/-)-Abscisic acid (ABA, CAS No:14375-45-2), and Gibberellic acid (GA3, CAS No: 77-06-5) were purchased from Merck KGaA/ Sigma-Aldrich (Darmstadt ...
-
bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
-
bioRxiv - Genomics 2021Quote: Cultures of three Lasiodiplodia and five Neofusicoccum species (Table 1) were inoculated onto cellophane covered 2 % malt extract agar (MEA; Biolab, Merck) and incubated at 22 °C ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
-
bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... The blocks were then washed with DDW 2 times for 10 min and incubated in 1% (w/v) uranyl acetate (Merck)/70% (v/v ...