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Citations for Merck :
2451 - 2500 of 5132 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v) (Merck, Roth) in a developing chamber (CAMAG ...
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bioRxiv - Biochemistry 2020Quote: ... One and a half μl of protein solutions were mixed on the target plate with 0.5 μl of the 20% 2,5-dihydroxybenzoic acid (Merck) solution in 20% ACN ...
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bioRxiv - Neuroscience 2020Quote: ... The larval samples were digested overnight at room temperature with concentrated (65%) nitric acid (Suprapur®grade, Merck) followed by 20 minutes at 90°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated in 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of the total lysates were mixed with 1mL 0.1 mg/mL pepsin in 0.5M acetic acid (Merck) and put at 4°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The separation was carried out using a reversed-phase Eclipse plus C 18 column (1.8 μm, 2.1 × 50 mm) with corresponding solvent A [0.1 % formic acid (FA; Merck, USA) in water] and solvent B [0.1 % FA in acetonitrile (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... prior to fixation embryonic zona pellucidae were removed in prewarmed (37°C) drops of Acid Tyrode’s solution (Merck) diluted in M2 ...
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bioRxiv - Cell Biology 2023Quote: ... overnight cultures were grown at 30 °C at 200 rpm in Yeast Nitrogen Base without amino acids (Merck) prepared according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 200 ng was loaded onto Evosep tips according to manufacturer’s instructions and separated using the pre-defined 30 samples per day protocol in combination with an Evosep 150 mm x 0.15mm C18 reversed phase column (EV1106 endurance column packed with 1.9 µm C18AQ particles from Dr Maisch) using 0.1% formic acid (Merck) in ultra-pure water (Biosolve ...
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bioRxiv - Cancer Biology 2024Quote: ... The final concentrations of fatty acids used in the HFHS medium were: 150 µM palmitate (Merck cat# P5585), 150 µM stearic acid (Thermo Scientific cat# A12244.06) ...
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bioRxiv - Biophysics 2024Quote: ... Modified amino acids Fmoc-Tyr(SO2ONp)-OH and Fmoc-Tyr(PO(OBzl)OH)-OH were purchased from Merck Life Science ...
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bioRxiv - Biochemistry 2024Quote: ... Phospho peptides were then reconstituted in 10 μl of MS loading buffer containing 0.3% Trifluoroactetic acid (TFA)(Merck) and 2% Acetonitrile (ACN ...
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bioRxiv - Microbiology 2019Quote: ... 6 μl of the reaction was spotted on foil backed silica plate (Silicagel 60, 20 x 20, Merck) and develop in butanol:acetic acid:water 2:1:1 (mobile phase) ...
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bioRxiv - Systems Biology 2020Quote: ... 400 µl were either filled into three wells of a 6-well TPP® tissue culture plate (Merck) supplemented with 0.5 % glucose and incubated under static conditions or used for inoculation of 3 × 10 mL TSB ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Cell Biology 2022Quote: ... then brought to 10 ml with ddH2O) and ECL2 solution (6 µl 30% (v/v) H2O2 (Merck, #H1009) in 10 ml with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: 6-7 dpf zebrafish larvæ were deeply anesthetized using 0.2% Ethyl3-aminobenzoate methanesulfonate (MS222; Merck KGaA, Darmstadt, Germany) diluted in EM ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% sodium dodecyl sulfate (SDS; AppliChem; Darmstadt, Germany) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II (Merck ...
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bioRxiv - Microbiology 2019Quote: ... IFNβ production was stimulated by transfecting cells with (10 μg/mL) polyinosinic: polycytidylic acid (poly I:C) (Sigma Aldrich, Merck). Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega) ...
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bioRxiv - Bioengineering 2022Quote: ... that was recently established by a disruption of both AAH1 and APT1.18 Yeast strains were grown overnight at 30 °C in a synthetic defined SD medium (6.7g/L Yeast Nitrogen Base without Amino Acids (Difco) and 20g/L glucose (Merck)) containing a specific mixture of amino acids and nucleobases ...
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bioRxiv - Bioengineering 2021Quote: ... The chemical reagents of L-cysteine (analytical reagent) and lactic acid (analytical reagent) were purchased from Merck (Darmstadt, Germany) and Fischer scientific (Merelbeke ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were washed three times with 1X PBS and IgG was eluted using 0.1M formic acid (Merck, Darmstadt, Germany) followed by immediate neutralisation with 1M ammonium bicarbonate (Acros Organics ...
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bioRxiv - Biochemistry 2020Quote: ... ammonia (25% in H2O), acetic acid (≥ 98.5%), ethanol (≥ 99.5%), and sodium hydrogencarbonate (NaHCO3, ≥ 99.5%) were purchased from Merck (Darmstadt, Germany). Tris(hydroxymethyl)amino-methane (TRIS ...
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bioRxiv - Microbiology 2020Quote: ... The LC flow rate was 300 nL/min and the 100-min linear gradient ranged from 95% solvent A (H2O with 0.1% formic acid (Merck)) to 40% solvent B (100% acetonitrile with 0.1% formic acid) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 10 minutes at room temperature, Ponceau (C.I.14700, FUJIFILM-Wako Pure Chemical Corp., Osaka, Japan) acid fuchsin (C.I.42685, Merck-Millipore) azofloxine (C.I.18050 ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the brain sections were thawed in a desiccator and spray-coated with 80 µl of a freshly prepared solution of 2,5-dihydroxybenzoic acid (DHB, Merck) using a SMALDIPrep ultrafine pneumatic spraying system (TransMIT GmbH) ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) and then transferred to 1.5 mL tubes prewarmed at 65°C acid-washed glass beads (G8772-100 Merck) suspended in 25 µL of 10% filtered SDS and 300 µL of acid phenol chloroform pH 4.5 ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant aliquots were kept at -18°C before being diluted with 2.5 mM sulfuric acid (H2SO4, Merck, Darmstadt, Germany) at 1:6 ...
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Neuroscience 2023Quote: ... mice were fasted for 6 hours prior to being given an IP bolus of 0.75 units/kg insulin (I9278, Merck). Blood was collected at 0 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviruses were added to cells together with 6 µg/ml of polybrene (TR-1003-G, Merck Milipore, Darmstadt, Germany) and incubated for 24 hours.
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
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bioRxiv - Immunology 2021Quote: ... The digested products were then diluted with formic acid to a final concentration of 0.1% and cleaned up using ZipTip C18 (Merck Millipore). Data was obtained on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... HPLC-grade ammonium formate (>98%) and formic acid (>98%) used in the LC mobile phase were purchased from Merck (Germany) and Sigma-Aldrich (USA) ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Microbiology 2020Quote: ... the supernatant was then filtered through an acid-washed 0.22 μm polycarbonate filter (GSV, Life Science) using a Sterifil 47 mm filter holder (Merck Millipore). The flow-through liquid was transferred into an acid-washed 10 L polycarbonate (PC ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Bioengineering 2022Quote: ... Nitric acid 65%, N,N-dimethylformamide (DMF), sodium hydroxide (NaOH) and Ethylenediamine (EDA, ≥99 %) were purchased from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4) at room temperature in a glass staining block ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...