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Citations for Merck :
2451 - 2500 of 3401 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... each coverslip was carefully removed from the plates and attached (side opposite to biofilm) on the surface of a regular glass slide using Entellan mounting medium (Merck, Darmstadt, Germany) for subsequent scanning as below.
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bioRxiv - Microbiology 2020Quote: Filter to remove host bacteria by transferring 200 µl (punch through pierceable tape) from each well to a 96-well filter Plate (0.45 µm) (e.g. MSHAS4510, Merck Millipore, Burlington MA US), pipette up and down a few times before extracting ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µL (= 5 mg fresh weight) of total lipids were applied onto a silica-coated chromatography plate (Silicagel 60 F254, 10x 20 cm; Merck, Rahway, NJ) and developed with hexane/ethyl ether/acetic acid (21 ...
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bioRxiv - Immunology 2024Quote: Thin-layer chromatographic (TLC) analysis of lipids was performed on chromatographic plates (TLC Silica gel 60 F254; Merck KGaA 64071, Darmstadt, Germany) according to our previously published protocol20.
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bioRxiv - Microbiology 2024Quote: ... standard dilution or sample was added into the wells of a 96-well clear bottom plate and 250 μL of Bradford reagent (Merck, Darmstadt, Germany) was added ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293A cells transfected with G-CASE and pcDNA or N-terminally FLAG-tagged H1/2R constructs were grown for 48 hours in transparent 96-well plates (Brand, Wertheim, Germany) and washed once with 0.5% BSA (Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Plant Biology 2023Quote: ... An aliquot of each sample was subjected to TLC using a silica gel 60 F254 aluminum TLC plate (Merck Millipore, Burlington, USA), using a running buffer containing ethyl acetate/acetic acid/methanol/formic acid/water at a ratio of 80:40:10:10:10 (v/v) ...
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bioRxiv - Synthetic Biology 2023Quote: ... All moisture-sensitive reactions were carried out in oven-dried glassware under N2 Thin-layer chromatograms (TLC) and flash chromatography separations were respectively performed on precoated silica gel 60 F254 plates (Merck, 0.25 mm) and on silica gel 60 (230-400 mesh) ...
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bioRxiv - Microbiology 2023Quote: Quantification of polar lipids content on HPTLC was carried out as follows: Lipid extracts and polar lipids standards at varying concentrations were applied onto silica gel 60 F254 HPTLC plates (Merck KGaA, Germany) using an ATS 5 automatic TLC sampler (Camag ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM DTT were heated in a LightCycler® 480 Multiwell white 384-well plate in a Roche LightCycler® 480 II from 20 to 80°C in the presence of SYPRO™ Orange (Merck) diluted 1:160.
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bioRxiv - Cell Biology 2020Quote: ... pDEST42-Ctdnep1_C-ter plasmid was transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore), Bacteria cultures (500 ml ...
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bioRxiv - Cell Biology 2020Quote: A549 cells were treated with 2 ng/ml TGF-β1 (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 2 days in DMEM with 10% FBS on non-treated culture dish surfaces (31 ...
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bioRxiv - Immunology 2020Quote: ... After each cycle the flow channels were regenerated for 10 seconds with 2 M NaCl (Merck) at 10 µL/min ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Bioengineering 2021Quote: ... with Pellicon 2 mini cassettes (300 kDa, 0.1 m2, BioMax polyethersulfone membrane with C-screen; Merck), as previously described (4) ...
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bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37°C and purified using a Amicon 30 kDa MWCO filter (Merck). Deep Vent exo-DNA polymerase (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... cells nuclei were stained with DAPI (4′,6-diamidino-2-fenilindol, 1:10000, Merck, cat#D9542) for 5 minutes at RT and mounted in Lab Vision™ PermaFluor™ Aqueous Mounting Medium (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in E.coli Rosetta-2 DE3 cells (EMD Millipore, Merck KGaA, Darmstadt, Germany). Cells were transformed via heat shock (42°C for 35 secs ...
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bioRxiv - Immunology 2021Quote: ... The left lung was inflated with 0.6mL PBS/2% (w/v) low melting point agarose (Merck) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were blocked with 2% BSA in PBS with 0.1% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in serum-free MEM supplemented with 2 µg/mL TPCK-treated trypsin (Merck) for 2 days at 37 °C in 5 % CO2 ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 10×106/100μL in PBS containing 2% FCS (Merck). Staining for cell surface antigens was performed for 20 minutes at 4°C in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified cells were cultured into organoids and treated with 2 μg/ml doxycycline (DOX, Merck, D9891) and 10 μM trimethoprim (TMP ...
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bioRxiv - Cancer Biology 2024Quote: ... counterstained with 5µg of 4’,6-diamidino-2-phenylindole (DAPI – 1:1) (Sigma/Merck, D9542-10mg) to eliminate dead cells before running through the flow cytometer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human Caco-2 colorectal adenocarcinoma cells (1.7×105 cells/cm2) were cultured in DMEM medium (Merck) containing 10% FCS at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Finally, clearing was performed in BABB (benzyl alcohol:benzyl benzoate 1:2, (Merck 8187011000 and Sigma 108006) for 48h.
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bioRxiv - Microbiology 2023Quote: ... One mL of culture broth was mixed in a 2:1 ratio with chloroform (Merck, Mumbai), followed by centrifugation (15,300 g ...
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bioRxiv - Biochemistry 2023Quote: ... in 30% (2-Hydroxypropyl)-β-cyclodextrin (HPβCD) or Sulfobutylether-β-Cyclodextrin (SBEβCD) (Sigma Aldrich, Merck, Germany). When tumors reached a size of 60-100 mm3 ...
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bioRxiv - Developmental Biology 2022Quote: ... During day 13 to 15 they were treated with 2 pulses of 3μM CHIR99021 (Merck, 361571) to dorsalise the tissue ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 μl of 2 mg/ml of 5ʹ-nucleotidase (snake venom from Crotalus atrox; Merck KGaA) in 0.1 M Tris-HCl pH 8.0 were added ...
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bioRxiv - Physiology 2023Quote: ... an amount of 2 g/kg body weight D-glucose (20% solution in PBS+/+, Merck, USA) was injected ...
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bioRxiv - Biophysics 2023Quote: ... 2 mL of supernatant containing viral particles was harvested and filtered with 0.45 μm filter (Merck). Supernatant was immediately used for transduction or stored at -80°C in aliquots.
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bioRxiv - Microbiology 2024Quote: One mL of culture broth was mixed in a 2:1 ratio with chloroform (Merck, Mumbai), followed by centrifugation (15,300 g ...
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bioRxiv - Physiology 2024Quote: ... The supernatant was filtered through glass wool and mixed with 2 liters of DEAE-sepharose (Merck) pre-equilibrated with Buffer A+ ...
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bioRxiv - Cell Biology 2024Quote: ... The loading pump supplied a solution of 0.1% trifluoroacetic acid in 98% water/acetonitrile 2% (Merck) at 3 µL/min ...
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bioRxiv - Synthetic Biology 2024Quote: ... 500 μl 0.2 μg/ml 4’,6’-diamidino-2-phenyl-indole (DAPI, Merck, catalog no. D9542) in PBS was added for 15 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:5,000; Merck Millipore) and covered with Mowiol (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: pCold-DDX43 and pCold-DDX6 were transformed into Rosetta 2 (DE3) competent cells (Merck Millipore/Novagen). The cells were cultured at 37°C until the OD600 reached ∼0.6 ...
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bioRxiv - Neuroscience 2024Quote: ... the neuronal microtubule cytoskeleton was labeled using rabbit anti-MAP-2 (Merck Millipore, #AB5622, 1:800). Following three washes ...
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bioRxiv - Bioengineering 2024Quote: ... followed by 10 min of crosslinking in a 2% (w/v) calcium chloride (CaCl2; Merck, Germany) solution at RT ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured with 50 U/ml of rhIL-2 in RPPMI-1640 medium (#8758, Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone ...
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bioRxiv - Cancer Biology 2024Quote: ... cells have been lysed using 2× Laemmli buffer supplied with 100mM DTT (Dithiothreitol, Merck Sigma Aldrich), boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
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bioRxiv - Biochemistry 2019Quote: ... The fractions corresponding to the single peak were pooled and concentrated using an Amicon Ultra −15 centrifugal filter (3 kDa, Merck Millipore Ltd. Ireland). Copper-(I ...