Labshake search
Citations for Merck :
201 - 250 of 613 citations for WesternBright MCF Fluorescent Western Blot Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).
-
bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were stirred using Teflon-coated magnetic stirrer bars and monitored by thin layer silica gel chromatography (TLC) using 0.25 mm silica gel 60F plates with fluorescent indicator from Merck. Plates were visualized under UV or treated by KMnO4 stain with gentle heating ...
-
bioRxiv - Microbiology 2021Quote: ... Fluorescent profiles were recorded using a Guava easyCyte flow cytometer and analyzed with InCyte software (Merck Millipore, Guyancourt, France).
-
bioRxiv - Developmental Biology 2021Quote: Fluorescent bead labeling solution was prepared by diluting 2µl aqueous suspension of fluorescent yellow-green beads (Diameter=1µm) (Merck L4655) in 8ml freshly filtered (0.2 μm ...
-
bioRxiv - Microbiology 2021Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
-
bioRxiv - Microbiology 2023Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... Calibration curves were acquired using defined amounts of the fluorescent product 4-methylumbelliferone sodium salt (#M1508, Sigma-Aldrich/Merck). Fluorescence of 4-methylumbelliferone was normalized to crystal violet intensity.
-
bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... anti-A17L (1:500) in combination with horseradish peroxidase (HRP)-coupled secondary antibodies (1:2,000) and Immoblion Forte Western HRP substrate (Merck) or with IR-Dye secondary antibodies (Licor ...
-
bioRxiv - Biochemistry 2019Quote: ... was used as a source of the secondary antibody and detected with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore) on Amersham Imager 600 (GE Healthcare).
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed thrice and bound antibodies were detected by using Immobilon ECL Ultra Western HRP Substrate (Merck Millipore) on the ChemiDoc XRS+ system.
-
bioRxiv - Cell Biology 2023Quote: ... Knockout of the molecules was confirmed by SDS-PAGE and western blotting using rabbit anti-ADAM10 polyclonal (Merck AB19026) at 1/500 followed by goat anti-rabbit HRP (BioRad ...
-
bioRxiv - Cancer Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
-
bioRxiv - Cell Biology 2023Quote: ... The immunoreactive bands were detected by using enhanced chemiluminescence (ECL) reagent (Immobilon Crescendo Western HRP substrate; Merck Millipore, DE). Band densities were quantified by densitometry using Image LabTM Version 6.0.1 2017 (Bio-Rad Laboratories ...
-
bioRxiv - Genomics 2023Quote: ... Western blotting with two additional antibodies was not successful: anti-trimethyl histone H3 lysine 27 (H3K27me3, Merck 07-449) (its 6 a.a ...
-
bioRxiv - Cell Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
-
bioRxiv - Biochemistry 2022Quote: ... Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin, Bovine Serum, 12659, Merck Millipore) powder solved in 1 × TBST ...
-
bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
-
bioRxiv - Immunology 2022Quote: Decidual cells were isolated as described above and incubated in the presence of fluorescence-labeled latex beads (50 beads per cell; latex beads, 1 µm, carboxylate-modified polystyrene, fluorescent yellow-green, Merck) for 2 hours at 37°C ...
-
mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore ...
-
bioRxiv - Immunology 2021Quote: ... was mixed 1:1 and incubated at RT for 2-3 min or ECL substrate is added (Immobilon crescendo western HRP substrate, WBLUR0100, Merck). Membranes were then exposed to film and developed or visualised by chemiluminescence using the G:BOX Chemi gel doc Imaging System Instrument (Syngene) ...
-
bioRxiv - Genetics 2021Quote: ... For western blotting the proteins were resolved in SDS-polyacrylamide gels and transferred electrophoretically at room temperature to PVDF membranes (Merck) for 1 h at 50 mA using a Tris-glycine buffer system ...
-
bioRxiv - Genetics 2021Quote: ... Prior to EMSAs the in vitro translations of Rbpj (WT) and mutant proteins were monitored by western blotting using an anti-Flag antibody (M5, Merck).
-
bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were detected with HRP-conjugated anti-rabbit or anti-mouse IgG and visualised with Immobilon Western HRP substrate (Merck).
-
bioRxiv - Genetics 2021Quote: ... Blots were washed thrice with TBS-T and visualized on a LAS4000 Fuji imaging system after incubating with Immobilon Western Chemiluminescent HRP substrate (Merck). The following antibodies were used ...
-
bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
-
bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the respective HRP-conjugated secondary antibody in 5% dry milk powder in TBS-T for 1h at room temperature washed three times in TBS-T and imaged on an ECL ChemoCam 3.2 Imager (Intas) using Immobilon Forte Western HRP substrate (Merck Millipore). For pulldown of gH-V5 or gH-V5/ gL-Flag complexes ...
-
bioRxiv - Cancer Biology 2022Quote: ... HRP substrate was applied (Lumi-Light Western Blotting Substrate cat#12015200001 from Sigma Aldrich, Immobilon Crescendo cat#WBLUR0020 from Merck Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
-
bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to a low fluorescent polyvinylidene difluoride membrane Immobilon-FL (0.45 μm pore size; Merck-Millipore, Czech Republic) by a Mini Trans-Blot® cell system (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
-
bioRxiv - Neuroscience 2023Quote: ... we applied with unlabeled (S)-PhPrM and (S)-PhPr solutions to the origin point on a reversed-phase plate coated with fluorescent indicator (RP-18 F254S, Merck Millipore). The developed spots were detected with ultraviolet light (254 nm).
-
bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
-
bioRxiv - Cell Biology 2020Quote: ... bound secondary antibodies were detected using either EZ-Chemiluminescence Reagent (Geneflow Ltd) or Luminata™ Forte Western HRP Substrate (Merck Millipore) and a Biospectrum 500 imaging system (UVP ...
-
bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
-
bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... and detected using primary and corresponding HRP-conjugated secondary antibodies on Fusion SL imaging system (Vibler) using Immobilon Western Chemiluminescent HRP Substrate (Merck, WBKLS0500). Molecular size of bands is marked in each panel [kDa] ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour with a species-appropriate HRP-conjugated secondary antibody (diluted 1:5000 in TBST) before bands were visualised using Immobilon Forte Western HRP Substrate (Merck Millipore). Primary antibodies used were mouse anti-αSMA (Dako ...
-
bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with goat anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody and the bands developed with Luminata Forte Western HRP Substrate (Merck Millipore).
-
bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed 3x in TBST for 10 min again and afterwards incubated in Immobilon ECL Ultra Western HRP Substrate (Merck, WBULS0100) for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
-
bioRxiv - Plant Biology 2022Quote: ... Secondary antibody and detection procedure was carried out following the manufacturer’s instructions (ECL™ Prime Western Blotting System, Merck; Darmstadt, Germany).
-
bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
-
bioRxiv - Cancer Biology 2023Quote: ... The signal was visualized using appropriate primary and secondary antibodies and the Immobilon™ Western Chemiluminescent HRP substrate (Merck, Darmstadt, Germany) and developed on an X-ray film ...
-
bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
-
bioRxiv - Neuroscience 2021Quote: ... the SH-SY5Y cells were collected and the ROS levels were detected using the fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA). Cells were incubated with 5 µM DHE dye for 20 min at 37°C in the dark and then the fluorescence intensity was measured by flow cytometry in the PE channel ...