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Citations for Merck :
201 - 250 of 1256 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... nsp16 and nsp7-11 were exchanged by six rounds of dilution and concentration in centrifugal filter units (Amicon Ultra, 10 K MWCO, Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Biophysics 2024Quote: Elution fractions containing the MS-ring composed of FliFG fusion proteins with the FliG-G214S or FliG-G215A mutations were concentrated 5-fold using an Amicon Ultra 100 K device (Merck Millipore). The G214S and G215A mutants were diluted 50- and 100-fold ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV titer was quantified usizg PCR (5′-TGA GTC ACC CAC ACA AAG GA-3′ and 5′-CCA AGC TGG CCT AAC TTC AG-3′) after proteinase K treatment (Merck Millipore). Under anesthesia with a mixture of medetomidine (0.3 mg/kg ...
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bioRxiv - Cell Biology 2024Quote: ... The cell culture medium was concentrated using Amicon® Ultra-15 3 K centrifugal filters (Merck Millipore Ltd., Burlington, MA, USA) into a final volume of 80 ml ...
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bioRxiv - Biophysics 2024Quote: ... Fractions containing FF A39G were concentrated and exchanged in the NMR buffer (100 mM sodium acetate, 200 mM sodium chloride, pH = 5.7) with Amicon Ultra 15 MWCO = 3 K (Merck, Darmstadt, Germany). The cleaved protein product was 71-residues long with no cloning artifacts at either end (GSQPAKKTYT WNTKEEAKQA FKELLKEKRV PSNASWEQGM KMIINDPRYS ALAKLSEKKQ AFNAYKVQTE K).
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bioRxiv - Cell Biology 2024Quote: ... This was achieved by buffer exchange with PBS buffer using Amicon® Ultra-0.5 3 K centrifugal filters (Merck Millipore Ltd.).
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bioRxiv - Neuroscience 2020Quote: ... RIPA and FA fractions was assessed using the Human Aβ1-42 enzyme-linked immunosorbent assay (ELISA) Kit (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Standard ligation of restriction enzyme digest products was performed using the Roche Rapid Ligation Kit (Merck Life Sciences). All reagents were used in accordance with the manufacturer’s specifications.
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bioRxiv - Microbiology 2021Quote: ... This crude enzyme solution was desalted using ultra centrifugal filters of 10 kDa cutoff value (Amicon Ultra, Merck) and then loaded on a 15 mm× 200 mm DEAE cellulose column pre-equilibrated with 20 mM Tris-Cl buffer (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: The enzyme assay couples the formation of ADP to NADH oxidation via PK (rabbit muscle, (Merck, Darmstadt, Germany)) and LDH (rabbit muscle ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin- labeled probes and biotin-labeled probes were detected with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/1,000 diluted streptavidin conjugated with DyLight 549 (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: Microvessels cultured under different flow conditions were perfused at day 7 or 14 with 70 kDa FITC-labelled dextran (Merck) by the application of a hydrostatic pressure drop across the central gel channel ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Biophysics 2021Quote: ... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
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bioRxiv - Biophysics 2021Quote: ... aSyn was extensively dialysed against H2O and used immediately for experiments or dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA, Darmstadt, Germany) and stored at −80 °C until use ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were combined and a buffer exchange and the protein were concentrated with a 30 K Amicon® Ultra Centrifugal Filter spin column (Merck KGaA) to the storage buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 100 mM NaCl) and concentrated down to 500 mL using an Amicon® Ultra-15 10 K Centrifuge Filter (Merck Millipore).
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bioRxiv - Microbiology 2024Quote: ... the SN was concentrated by ultrafiltration at 4000□g and 4°C using 100 K Amicon® Ultra-15 centrifugal filters (Merck Millipore). The concentrated SN was filtered through a 0.45-µm-pore-size filter ...
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bioRxiv - Cell Biology 2024Quote: ... cellular morphology was documented in HL-60 and K-562 by staining with 4-Nitro blue tetrazolium chloride (NBT) (James et al., 1997) () or Neutral Red solution (Merck/Sigma-Aldrich) (Russo et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... The enzymes were concentrated to 10-19.5 mg ml−1 with Amicon Ultra 10k and 30k MWCO centrifugal filters (Merck), flash frozen in liquid nitrogen and stored directly at −80°C ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was digested with restriction enzymes and then ligated into pET-17b (Merck Millipore, Burlington, MA, USA) to construct the expression vector pStSOR ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Developmental Biology 2024Quote: ... the lung distal regions were cut into small pieces and dissociated in 5 mL of enzyme mixture (0.125 mg/mL Collagenase, Merck, C9891 ...
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bioRxiv - Cell Biology 2024Quote: ... Medium in the capillary channel was then replaced with serum free endothelial cell media containing 40 mg/ml FITC-albumin (Merck, A9771), and the non-cellular channel medium replaced with serum-free RPMI ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were stopped by separating the enzyme and soluble products from the insoluble chitin substrate by filtration using 96-well filter plates (Merck). The concentration of oxidized LPMO products was also determined in aliquots taken from the pre-incubation reaction with β-chitin to allow accounting for carry-over of products potentially formed in the pre-incubation phase.
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bioRxiv - Developmental Biology 2023Quote: Upper and lower arms were harvested from 4 cm double reporter axolotls (Alx4:mCherry_Hand2:EGFP) and dissociated into single cell suspensions using Liberase TM enzyme (Merck 05401127001) as described in 49 ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
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bioRxiv - Biophysics 2024Quote: ... Primary anti-KI67 antibody (Merck, rabbit polyclonal antibody) was diluted 1:300 in antibody buffer (0.2 % Tween 20 ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... enzymes were concentrated by centrifugation in Centriprep® 30K and Amicon® Ultra-4 Ultracel®-10K devices (Merck Millipore Ltd). Purity of enzymes was assessed by SDS–polyacrylamide gel electrophoresis ...
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Low-cost drug discovery with engineered E. coli reveals an anti-mycobacterial activity of benazeprilbioRxiv - Synthetic Biology 2021Quote: ... in which L-alanine produced by ALR drives the stoichiometric production fluorescence-readable NADH through the activity of the coupling enzyme L-alanine dehydrogenase (Merck A7189).
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bioRxiv - Molecular Biology 2021Quote: ... 300-500bp homology fragments were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Merck Life Science). N-terminal FKBP12F36V fragments were originally amplified originally from pLEX_305-N-dTAG (Addgene #91797 ...
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bioRxiv - Microbiology 2024Quote: The activity of the superoxide dismutase (SOD) enzyme was measured in cell lysates using the SOD Determination Kit (Sigma Merck®), and calculations were carried out following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Non-infected and MCMV-infected mice were orally gavaged with 150 μl of 100 mg/ml 4 kDa FITC-dextran-labeled dextran beads (Sigma-Aldrich/Merck, Darmstadt, Germany) in PBS 4 hours prior to sacrifice ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
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bioRxiv - Biochemistry 2022Quote: ... [2H7]22R-Hydroxycholest-4-en-3-one ([2H7]22R- HCO) was prepared from [2H7]22R-HC by treatment with cholesterol oxidase enzyme (Streptomyces sp., Merck, Dorset, UK) (11).
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...
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bioRxiv - Plant Biology 2022Quote: ... GSL in 1 ml crude extracts were bound on 250 µl DEAE-Sephadex A-25 columns and digested on the column for 18 h at room temperature with 18U sulfatase H1 enzyme (Merck KGaA, Germany). Desulfoglucosinolates were eluted twice with 1 ml ddH2O ...
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bioRxiv - Microbiology 2022Quote: ... At least 5,000 cells were counted per run and FITC-positive cells were detected with a FlowSight imaging flow cytometer (Amins, Merck Millipore, Billerica, MA, USA) equipped with lasers at 405 nm (violet) ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibody (Merck guinea pig anti-NeuN 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were mouse anti-EGFR antibody (#GR01; Merck Millipore), goat anti-EEA1 (#sc-6414 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody RNA-DNA hybrid (s9.6) antibody (Merck Millipore, #MABE1095, 3ul) or GFP (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Biochemistry 2020Quote: ... The enzyme was then removed via diafiltration by passing the mixture through a 30 kDa centrifugal filter device (Amicon®, Merck Millipore Ltd., Ireland). The absence of AtXOAT1 in the filtrate ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...