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Citations for Merck :
201 - 232 of 232 citations for Recombinant Rat Pdcd1 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 µg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA] + 10% fetal calf serum [FCS; Merck, Darmstadt, Germany]).
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... urinary human menopausal gonadotropin (hMG) (HMG-lepori 75 UI, Angelini Farmacéutica, S.A, Spain) and recombinant hCG (Ovitrelle 250 micrograms, Merck Serono Europe Limited, Spain) or luprorelin acetate (Procrin 1 mg/0,2 ml ...
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bioRxiv - Bioengineering 2021Quote: ... rats were sacrificed via injections of pentobarbital/phenytoin at 150 mg/kg pentobarbital (Beuthanasia®-D C IIIN, Merck Animal Health ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each group was then further split into two subgroups depending on whether the rats received bicuculline (Merck, Darmstadt, Germany) or vehicle (artificial cerebrospinal fluid ...
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bioRxiv - Bioengineering 2021Quote: ... the chemically decellularized aortas were treated with 900 units (25 μg/mL DNase I recombinant, RNase free, Cat. No. 4716728001, Roche, Merck KGaA, Darmstadt, Germany) using a total of 3 DNase treatment for 2 hours each to achieve the desired level of DNA assessed by (Quant-iT™ PicoGreen™ dsDNA Assay Kit ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... while the rat abdominal aortic rings (15 mg wet mass/mL) were pre-incubated for 10 min in Medium 199 (Merck). The examined enzyme reaction started after pipetting the tracer substrate 1-14C-AA (3.7 kBq ...
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bioRxiv - Neuroscience 2020Quote: ... A single intravitreal injection of ML240 or EerI to all P23H rats previously anesthetized (ST and LT: Diethyl ether, Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Biophysics 2021Quote: Cultures of dissociated rat hippocampal primary neurons were prepared from postnatal P0-P2 Wistar rats of either sex and cultured on glass coverslips coated with 100 µg/mL poly-ornithine (Merck KGaA) and 1 µg /mL laminin (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The chemoreflex was activated by systemic bolus intravenous injections of 0.1 mL of KCN (40 μg per rat; Merck, Darmstadt, Germany) following procedures described by Franchini & Krieger (1993 ...
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bioRxiv - Immunology 2021Quote: ... Freshly isolated cells (4 x 106) were seeded in a pre-prepared gel matrix containing 1 mg/mL rat tail collagen type I (Merck Millipore) in DMEM ...
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bioRxiv - Microbiology 2022Quote: ... Cytokine and chemokine levels were measured with a commercial rat cytokine/chemokine magnetic bead panel 96-well plate assay kit (Milliplex MAP kit, Merck Millipore), which detects 5 cytokines and chemokines including IP-10/CXCL10 ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: Astrocytes for long-term neuronal cultures were prepared by dissociating newborn rat cortices with 2.5% trypsin and 1 mg/ml DNase (Merck, cat# 10104159001) and plating the cells in MEM with L-glutamine supplemented with 0.6% glucose ...
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bioRxiv - Neuroscience 2023Quote: ... rats were anesthetized and underwent transcardial perfusion with 100 mL of saline followed by 100 mL of 4% paraformaldehyde (Merck, Germany) at 72 h following ICH induction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and follicle stimulating hormone (FSH) were determined using MILLIPLEX MAP Rat Pituitary Magnetic Bead Panel following manufacturer’s protocol (Merck-Millipore, Burlington, MA). Serum free fatty acids were measured using kits from Cell Biolabs ...
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bioRxiv - Biophysics 2019Quote: The DNA sequence encoding for the ARD of rat TRPV1 (residues 100-262) was cloned into a pET28c vector (Merck-Millipore, USA). For expression ...
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253, Darmstadt, Germany), 1:25000 diluted primary mouse monoclonal anti-beta actin antibody (AC-15 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the cell lysates was measured using the BCA assay and insulin levels were quantified by radioimmunoassay (rat insulin RIA kit, Merck, Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Microbiology 2019Quote: ... were maintained at 37°C on rat collagen diluted at 100µg/mL in water (Cultrex; catalog no. 3443-100-01) in EndoGro medium (Merck Millipore; catalog no. SCME004) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... rat polyclonal anti-Vangl2 (clone 2G4, 1:200 for Western blotting, 1:400 for immunoprecipitation, 1:500 for immunostaining, Merck Millipore, catalog no. MABN750), mouse monoclonal IgG1k anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells (primary culture of rat ENS) and tissues from mice (hippocampus, ileum, proximal and distal colon) were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing phosphatase inhibitor cocktail II (Roche ...
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bioRxiv - Genetics 2022Quote: ... Alendronate 1.0 ug/kg body weight was subcutaneously injected into rats in ALN group daily (alendronate sodium pure dry, provided by Merck and Co., Inc., Rahway, NJ, USA) (Diab ...