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Citations for Merck :
201 - 250 of 1335 citations for Recombinant Bovine FCGRT & B2M Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... with 10% (vol/vol) fetal bovine serum (Merck, F9665-500mL), 2CmM L-glutamine (Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% fetal bovine serum (Merck Life Science NV) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were blocked with 1% bovine serum albumin (BSA; Merck), 5 % normal goat serum (NGS ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% Foetal bovine serum (FBS) (Merck, F4135-500ML), 100 IU/ml pencillin and 100 µg / ml streptomycin (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were blocked with 3% bovine serum albumin (Merck) in PBST at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2% fetal bovine serum (FBS, heat inactivated, Merck), 5 µg/ml insulin (Merck ...
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bioRxiv - Biophysics 2020Quote: ... fluorescently-labeled and biotinylated H57-scFV were again concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK) and stored in PBS supplemented with 50% glycerol at −20°C ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented by 10 % fetal bovine serum (FBS) (Sigma/Merck, Darmstadt, Germany) and 1 % Penicillin/Streptomycin (Gibco/Thermo Fisher scientific ...
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bioRxiv - Neuroscience 2020Quote: ... slices were blocked with 20% bovine serum albumin (BSA, Sigma, Merck) and PBS-0.3% Triton X-100 for a minimum of 4hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Germany) supplemented with 10 % calcium-free foetal bovine serum (S0615, Merck), 2 mmol/l stable glutamine (BioConcept ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal bovine serum (FBS; Biochrom/Merck, Schaffhausen, Switzerland) at 37°C and 5% CO2 ...
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bioRxiv - Plant Biology 2022Quote: ... To this was added 10 mM bovine bile salts (Merck B3883), 0.6 mM CaCl2(H2O)2 ...
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bioRxiv - Neuroscience 2021Quote: ... except that B27 was replaced with 10% fetal bovine serum (Merck) and 2.5S NGF was lowered to 30 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... with added 10% foetal bovine serum (FBS) (Sigma-Aldrich, Merck, KGaA), 2 mM glutamine (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... 1% (w/v) fatty acid-free bovine serum albumin (BSA, Merck) and 1x EDTA-free cOmplete™ protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% (vol/vol) fetal bovine serum (FBS) (Merck, F9665), 2 mM L-glutamine (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 15% EmbryoMax fetal bovine serum (ES-FBS Merck-Millipore) and 20 ng/ml leukemia inhibitory factor (LIF ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were blocked with 3% bovine serum albumin (Merck Aldrich) in PBST at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... media was supplemented with 10% heat inactivated Fetal Bovine Serum (FBS, MERCK), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Cell Biology 2021Quote: ... in D-PBS supplemented with 1% bovine serum albumin (BSA, Merck, 05470). Primary antibodies diluted in blocking buffer were incubated with the cells at RT for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspended in 1X PBS with 0.05% Bovine Serum Albumin (BSA; Merck, 05479), and passed through a 35μm filter (Corning ...
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bioRxiv - Biophysics 2021Quote: ... and 10% (v/v) fetal bovine serum (Merck Millipore, Burlington, MA, USA). U-2 OS cells (ATCC ...
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bioRxiv - Physiology 2022Quote: ... SFM was replaced with SFM containing 2 % bovine serum albumin (BSA) (Merck). To stimulate the βARs ...
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bioRxiv - Microbiology 2022Quote: ... The membrane were blocked with 5% bovine serum albumin (Merck Millipore, USA) in TBST (1x Tris-Buffered Saline ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: Two milligrams of insoluble fibrillar collagen from bovine Achilles tendon (Merck, Germany) were added to an Nanosep microcentrifugal device (0.2 µm pore size ...
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bioRxiv - Bioengineering 2023Quote: ... They were then incubated with a 3% bovine serum albumin (BSA, Merck)/ 0.1% Triton X-100 solution in PBS for 2 h at RT and subsequently overnight at 4 °C with primary or conjugated antibodies in a 1% BSA/ 0.1% Triton X-100 solution ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, South America, Merck, Darmstadt, DE), 1% L-glutamine and 1% penicillin–streptomycin (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... 0.05% TWEEN-20) supplemented with 200–400 U bovine DNase I (Merck) and 200 μL EDTA-free protease inhibitor cocktail (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...