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Citations for Merck :
201 - 250 of 1264 citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were visualised on Licor Odyssey Fc (Cambridge, UK) using enhanced chemiluminescence (ECL, Merck Millipore, Darmstadt, Germany). β-actin was used a loading control.
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Plant Biology 2023Quote: ... the sequence of CBL1 was fused with an N-terminal 2xStrepII tag in the pET-24b vector (Merck, Darmstadt, Germany). Sequences of active CPK5 and CIPK26 were fused with an N-terminal 2xStrepII tag in the pIVEX-1.3WG vector (Biotechrabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Cell Biology 2019Quote: ... 2% peptone (Becton Dickinson) and 2% glucose (Merck, Darmstadt, Germany). SC consisted of 0.7% yeast nitrogen base (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 2% peptone (Becton Dickinson) and 2% glucose (Merck, Darmstadt, Germany). SCD-MSG medium consisted of 0.17% yeast nitrogen base without amino acids and ammonium sulfate (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Biophysics 2021Quote: ... Human pancreatic beta cells 1.4E7 were purchased from Merck and cultured in RPMI-1640 medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... conidia were opsonized with normal human serum (Merck Millipore). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... human mitochondria (clone 113-1, Merck Millipore, 1:100), and BrdU (clone BU1/75 ICR1 ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml human EGF (Merck, Cat Number: E9644), 0.5 mg/ml hydrocortisone (Merck ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg/mL mouse anti-human TGN46 (SAB4200355, Merck), or a 1 in 20 dilution of rat anti-β-tubulin (clone YL1/2 ...
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bioRxiv - Biophysics 2021Quote: Rabbit polyclonal anti human IRSp53 (07-786 – Merck Millipore), mouse monoclonal anti CA (24.2 – NIH AIDS Reagent Program – FisherBioServices) ...
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bioRxiv - Biophysics 2021Quote: ... rabbit polyclonal anti human IRSp53 (07-786 – Merck Millipore), mouse anti CA (183H125C – NIH3537) ...
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bioRxiv - Bioengineering 2021Quote: The human immortal chondrocyte cell line – C28/I2 (Merck) was used in this study ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mg/mL Human recombinant Insulin (Merck, Cat. #91077C) and 1% Pen/Strep ...
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.2 IU/ml human chorionic gonadotropin (HCG) (C1063, Merck), 25 µg/ml sodium pyruvate and penicillin-streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg/ml of IgG from human serum (Merck) in PBS were added to all blocking and antibody incubations ...
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bioRxiv - Cell Biology 2023Quote: ... were coated with human plasma fibronectin (Merck Millipore FC010) at a concentration of 5 µg/cm2 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 IU/ml recombinant human FSH (Merck & Co., Inc), 0.125 mg/ml recombinant human hyaluronan (Novozymes) ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-human IgG (AP309P, Merck KGaA). For stripping and re-probing of Western blot membranes ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore), and anti-E-cadherin-1 (ECAD ...
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bioRxiv - Microbiology 2024Quote: ... the bacterial suspension and human serum (Merck, Darmstadt, Germany) were mixed in a 1:1 ratio (100 µL each) ...
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bioRxiv - Cell Biology 2024Quote: ... 50nM Mission esiRNA against human PTGER2 or PTGER4 (Merck). 50nM non-targeting Silencer™ Negative Control #1 siRNA (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Merck), JQ-1 (Sigma Aldrich) ...
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bioRxiv - Genomics 2024Quote: ... Cells were then resuspended in 100µL RPMI + 10% FCS and stimulated for 4h with PMA (100ng/mL, Merck, P8139) and ionomycin (1µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat. #A05174 and Human Adiponectin ELISA, Merck, Cat. # EZHADP-61K). The minimum detectable dose of leptin was 7.8 pg/mL and that of adiponectin was 0.891 μg/mL.
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bioRxiv - Biochemistry 2020Quote: ... was fused to an N-terminal TEV-cleavable His6-GCN4 tag (as described in Sosa et al. (2012)) and cloned into a pRSF-Duet1 (Merck Millipore) vector ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck, or #14793 Cell signaling ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: The SUN domain sequence of human SUN1 (amino-acids 616-812) was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Plant Biology 2023Quote: ... a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa, Merck).
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bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Cancer Biology 2021Quote: ... cells in IMDM+Glutamax medium were cryopreserved in liquid nitrogen until time of analysis complemented 1:1 with 80% FCS and 20% dimethyl sulfoxide (DMSO) (Merck).
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bioRxiv - Immunology 2023Quote: ... for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest) and 10% DMSO (Merck) using a cell freezing container (CoolCell LX ...
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bioRxiv - Immunology 2021Quote: ... Human pancreatic beta cell line 1.4E7 was purchased from Merck and cultured in RPMI-1640 medium (Thermo Fisher Scientific ...