Labshake search
Citations for Merck :
201 - 250 of 1621 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The floated sections were rinsed briefly with 100 mM maleate buffer (pH 7.5) and blocked for 30 minutes against non-specific antibody binding with 2% blocking reagent (Merck) in maleate buffer ...
-
bioRxiv - Neuroscience 2019Quote: ... Slices were then permeabilized in blocking solution containing 5% normal goat serum (NGS, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in 0.5% Triton for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... were incubated for 24 hours in blocking buffer containing 10% (vol/vol) normal donkey serum (NDS) (S30-100ML, Merck), 0.5% (vol/vol ...
-
bioRxiv - Cell Biology 2022Quote: ... The buffers used during the whole procedure had fish gelatine 1.6% (v/v) as a blocking agent (G7765, Merck). The primary antibodies used were anti-NR1D1 (ab174309 ...
-
bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... All primary and secondary antibodies were diluted in a blocking solution consisting of PBS and 0.2% (v/v) bovine serum albumin (BSA, Merck). Cells were first washed with ice-cold PBS and incubated for 5 min in a blocking solution on ice ...
-
bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
-
bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
-
bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
-
bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
-
bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
-
bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck), 100 µM jasplakinolide (Abcam ...
-
bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM sodium pyruvate (Merck TMS-005-C), 2.5 g/L glucose (Sigma G8769) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mitomycin C (10 g/mL) (Merck, Darmstadt, Germany) was then used to treat the cells for two hours and using the tip of a 200 µL pipette ...
-
bioRxiv - Neuroscience 2023Quote: ... c- Fos anti-rabbit (1:200, Merck, ABE457), Gephyrin anti-Mouse-IgG1 (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... 4 °C in 10 kDa Amicon (Merck Millipore) filters ...
-
bioRxiv - Cell Biology 2023Quote: ... and 60 μg/ml catalase (Merck, C-40).
-
bioRxiv - Plant Biology 2023Quote: ... 4°C (Merck 3-16KL, KGaA®, Germany). Supernatant was collected and filtered through PTFE membrane filter (0.2 μm ...
-
bioRxiv - Neuroscience 2024Quote: ... Laminin A/C (#SAB4200236, Merck or #ab108595, Abcam), LRP-1 (#ab92544 ...
-
bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
-
bioRxiv - Developmental Biology 2021Quote: ... They were incubated 1h at RT in a blocking buffer (20% BR + 20% goat serum) and then overnight with an anti-DIG-AP antibody (1:2000, Merck) in the blocking buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... MDMi cells were incubated with a primary antibody against Iba1 (1:1,000; 019-19744, FUJIFILM Wako Chemicals) in blocking buffer (2% bovine serum albumin, 0.1% TritonX100 (Merck, Darmstadt, Germany), and 5% normal donkey serum (017-000-121 ...
-
bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody anti-Rabbit or/and goat IgG antibody conjugated with HRP (GENA934, AP106P, Merck) at room temperature for 1 hour for enhanced chemiluminescence ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were caught and rinsed in 1x PBS (3 × 15 min) prior to incubation in blocking solution (5% NDS (Merck) in 0.3% PBS-Triton-X-100 ...
-
bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
-
bioRxiv - Pathology 2023Quote: ... blocked with normal serum for 1 h at 22–24°C and incubated overnight at 4°C with rabbit polyclonal anti-Lubricin antibody (1:500, MABT401, MERCK, DEU). The sections were then washed three times with PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 minutes, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 minutes, 4°C). After washing ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Single product was validated on 1.5% agarose gel after incubated at 95 °C for two minutes in thermo cycler with hot lid then brought to 22 °C and mixed with formamide (Merck millipore) in 1:3 ratio ...
-
bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...
-
bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
-
bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
-
bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
-
bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
-
bioRxiv - Cell Biology 2023Quote: ... and were treated with various concentrations of peptides (as determined by prior Hoechst 33258 staining in serum–free media) or solvent (DMSO, 0.1% v/v, Merck) or positive control TGF-β1 10ng/ml (Sigma ...
-
bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
-
bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...