Labshake search
Citations for Merck :
201 - 250 of 1902 citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Cell Biology 2023Quote: ... each sample was incubated for 12 h (or overnight) in bleaching solution containing hydrogen peroxide solution (H2O2; Cat. No. H1009; Sigma-Aldrich, Merck, Germany) at a final concentration of 15% (v/v)) ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... a cell viability assay was performed after the luciferase assay by adding 12 μL of resazurin (Merck Life Sciences, Cat #: TOX8-1KT) and the cells incubated for an additional 2 hours followed by fluorescence reading on a Promega Glomax multimode plate reader.
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng recombinant H6-EIF4EBP1 (cloned and purified as described above), 500 mM AZD8055 (MedChem Express, HY-50706) and 0.133 mM ATP (Merck, 74804-12-9).
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bioRxiv - Microbiology 2024Quote: ... the water samples were filtered gently using a volume pump (<300 mbar) and a 12 µm cellulose filter (WHA10400012, Whatman, Merck, Darmstadt, Germany). The collected material (particles of ≥12 µm) ...
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bioRxiv - Immunology 2024Quote: ... the cells were stimulated with 500 nM ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) (both from Merck Sigma-Aldrich), and BD GolgiPlug Protein Transport Inhibitor (containing Brefeldin A ...
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bioRxiv - Biochemistry 2024Quote: ... 5x 105 cells/well were seeded into 6-well plates and incubated with 20 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma/Merck, Darmstadt, Germany) in RPMI1640 medium containing 10% v/v heat-inactivated FCS for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... OVCAR8 landing pad lines were generated by incubating 0.5×106 OVCAR8 cells per well in a 12-well plate with viral supernatant diluted ¼ into culture media with 8 µg/ml polybrene (Merck, Cat #H9268) and spun for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were dissociated by mincing and enzymatic digestion of the extracellular matrix using 500 µg/ml collagenase type 1a (Merck Life Sciences UK Ltd, Gillingham, U.K) and 100 µg/ml DNAse I (Lorne Laboratories Ltd ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Flash chromatography was performed on a Varian 971-FP flash purification system using silica gel cartridges (Varian, particle size 50 μm) or on glass columns using silica gel type 60 (Merck, particle size 230 μm, 400 mesh). All compounds were obtained as oils ...
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bioRxiv - Cell Biology 2024Quote: ... and EnSCs were dissociated from endometrial biopsies by enzymatic digestion of the extracellular matrix using 500 µg/mL collagenase type Ia (Merck Life Sciences UK Ltd, Gillingham, UK) and 100 µg/mL DNase I (Lorne Laboratories Ltd ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... Suspensions were passed through a 40-µm strainer into a 50 ml tube and rinsed again in 12 ml of DMEM with 10% Fetal bovine serum (FBS) (Merck, Kenilworth New Jersey) in 15 ml tubes ...
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Zoology 2020Quote: ... Wing scales were removed using the methods published Carvajal et al.12 and were then mounted on a glass slide and cover slip with Aquatex® (Merck, Darmstadt, Germany). Wing images were captured with 3.5x magnification using Nikon SMZ800 dissecting microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 1,500 worms per replicate were harvested at 12 h post L4 and washed across a Steriflip® 20 μm nylon mesh filter (Merck; Darmstadt, Germany) with M9 buffer ...
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bioRxiv - Bioengineering 2024Quote: ... cells were cultured under standard cell culture conditions in FBS-free RPMI-1640 cell culture medium supplemented with 300 nM phorbol 12-myristate 13-acetate (PMA; Merck KGaA, Darmstadt, Germany) for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Microbiology 2021Quote: Colorimetric pH measurement was performed for 12 independent pancreatic juice samples by applying 1 µL sample onto MColorpHast™ pH strips (Merck Millipore, MA, USA). To adjust the pH of pancreatic juice ...
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bioRxiv - Microbiology 2021Quote: ... All viromes were concentrated from 12 ml down to approximately 200 μl SM-buffer using the Amicon® Ultra-15 Centrifugal Filter Devices 100K (Merck Millipore, Darmstadt, Germany), following manufactures recommendations ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Pathology 2021Quote: ... mouse anti-phospho-H2AX and rabbit anti-S100A4 (Merck Millipore), rabbit anti-CD31 and rabbit anti-VEGFA (Abcam) ...
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bioRxiv - Genomics 2022Quote: ... Anti-IgG (ABIN101961) or anti-HA (05-902R, Merck Millipore) antibodies were used ...
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bioRxiv - Genomics 2022Quote: ... anti-Pol II (8WG16) and anti-H3 (Merck, 07-690) antibodies were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-actin (A2066) and anti-puromycin antibodies (clone 12D10, Merck) were purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 5×105 cells/well were seeded in 48-well plates and incubated in RPMIc with 200nM PMA (Phorbol 12-myristate 13-acetate, Merck Sigma, Saint Quentin Fallavier, France). After 24 h ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-Tubulin (Merck), anti-Bcl-2 ...
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bioRxiv - Physiology 2022Quote: ... anti-Smpd3 (Merck clone 14G5.1 ...
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bioRxiv - Microbiology 2022Quote: ... anti-SRSF1 (Merck) and anti-GFP (ABio laboratories) ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... anti-CREB3L1 (AF4080; R&Dsystems) or anti-H3K27me3 (07-449, Merck). Detection was performed with goat anti-rabbit DyLight-594 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse monoclonal anti-tubulin anti-body (Merck, 3272293, MAB3408, 1:10,000), Rabbit polyclonal anti-dMi-2 anti-body (custom made ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2020Quote: ... anti-Aβ40 and anti-Aβ42 primary antibodies were purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Neuroscience 2020Quote: ... anti-Aβ40 and anti-Aβ42 were from Merck (Kenilworth, MJ, USA); anti-Aβ clone 6E10 (803001 ...
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bioRxiv - Plant Biology 2023Quote: ... anti-mouse or anti-chicken IgG (diluted 1:30 000, Merck), respectively ...
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bioRxiv - Immunology 2024Quote: ... anti-CD3 and anti-CD28 antibody coated bead (Merck, Darmstadt, Germany) based stimulation for 24 or 48h ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight incubation at 4°C with primary antibodies (Anti-ASL, Abcam ab97370, 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233 ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).
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bioRxiv - Immunology 2023Quote: ... and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG, #A0545). Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG immunoprecipitations were performed using anti-FLAG M2 magnetic beads (Merck). All beads were washed 3x in lysis buffer prior to use ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GAPDH (Merck), and rabbit anti-actin (Santa Cruz ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Genomics 2020Quote: ... anti-Tyr1P (3D12; Merck), anti-H3K4me3 (ab8580 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-HOIP (Merck SAB2102031), anti-IκBα (44D4 ...