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Citations for Merck :
2401 - 2450 of 3054 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... solution for 30 min at RT and washed for 20min in a 10% formamide (Merck, #F9037), 2X SSC (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with branched polyethylenimine (bPEI, 10 μM stock solution, Merck, Sigma-Aldrich, Darmstadt, Germany) when 90% confluency was reached ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 hour with 10 µg/mL Calcofluor White M2R (CFW) (Merck KGaA). Cells were harvested by centrifugation (2,000 ×g ...
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bioRxiv - Microbiology 2024Quote: ... v/v) and applied on glass backed silica gel plates (60, 10 × 20 cm; Merck, #1.05626.0001). Plates were developed in chloroform/ethanol/triethylamine/ddH2O 30/35/35/7 (v/v/v/v ...
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bioRxiv - Bioengineering 2024Quote: ... followed by 10 min of crosslinking in a 2% (w/v) calcium chloride (CaCl2; Merck, Germany) solution at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 10 to 20 minutes column incubation with 14 mL of ultrapure water (Merck Milli-Q) as the eluant for the final step ...
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bioRxiv - Immunology 2024Quote: ... in some cases supplemented with combinations of UEA1 Atto 488-conjugated lectin (10 µg/mL; Merck), UEA1 DyLight647-conjugated lectin (10 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Total supernatant (S1 + S2) was passed through a 10 μm hydrophobic PTFE filter (LCWP02500, Merck Millipore) and centrifuged for 1 min at 4,000 g ...
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bioRxiv - Neuroscience 2024Quote: ... The male and female treated groups (FE and ME) received 10 nM β-estradiol (Merck, E2758), dissolved in 100% ethanol further diluted in culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate, and 10% acetonitrile (Merck; cat. #1.00029). Gel fragments were dehydrated with pure acetonitrile for peptides extraction and the recovered supernatants were totally dried in a vacuum concentrator (SpeedVac) ...
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bioRxiv - Molecular Biology 2024Quote: ... the eluates were concentrated in Vivaspin 500 centrifugal concentrators with 10 kDa cut-off (Merck; Z614033). To elute proteins from IP samples ...
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bioRxiv - Cancer Biology 2024Quote: ... Brains were collected immediately after sacrifice and fixed in 10% Neutral Buffered Formalin (Merck; HT501128-4L) overnight.
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bioRxiv - Genomics 2024Quote: CD spectroscopy measurements were conducted on a Chirascan V100 (AppliedPhotophysics) using 10 μM DNA probes (Merck) in 270 μl (Table 4 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM Imidazole (Buffer A) supplemented with 1:1000 protease inhibitors (Calbiochem Set III, Merck 539134), 3 µg/mL Benzonase-MBP ...
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Plant Biology 2020Quote: About 8,000 T4 seeds from the W29-1-4 reporter line were mutagenized by treatment with 0.3% (v/v) ethylmethanesulfonate (Merck KGaA, Frankfurter, Darmstadt, Germany) for 15 h at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Physiology 2021Quote: ... All incubations with primary antibodies were performed for 16 hours at 4°C. Blots were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
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bioRxiv - Plant Biology 2020Quote: ... Conidia for either seeding liquid cultures or for use in pathogenicity assessments were collected by washing 4-week-old MM cultures with sterile water (SW, Milli-Q Integral Water Purification System for Ultrapure water, Merck KGaA, MA, USA), then filtered through glass wool to remove mycelial debris ...
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bioRxiv - Neuroscience 2021Quote: ... the cells and supernatants were harvested and purified using chloroform and were condensed using Amicon Ultra-4 Centrifugal Filter Devices (100K MWCO; Merck Millipore, Billerica, MA).
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bioRxiv - Cell Biology 2022Quote: ... centrifuged at 3,000 × g at 4°C and filtered under sterile conditions using a 0.22 μm filter (Millex-GV, PVDF; Merck/Millipore Watford, Hertfordshire, UK), and is referred to as CM-OAT1.
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bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck, Buchs Switzerland). Memofl/fl littermates without Cre but treated with tamoxifen served as controls ...
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bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Microbiology 2022Quote: Non-infected and MCMV-infected mice were orally gavaged with 150 μl of 100 mg/ml 4 kDa FITC-dextran-labeled dextran beads (Sigma-Aldrich/Merck, Darmstadt, Germany) in PBS 4 hours prior to sacrifice ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were clarified by centrifugation at 12000g and incubated 2h at 4°C with pre-equilibrated α-FLAG M2 affinity gel beads (Merck-Sigma, A2220). Beads were washed three times with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... Residues in solution were pelleted by centrifugation at 12000Xg for 30 minutes at 4℃ and soluble fraction was filtered through 0.22-micron filters (Merck Millipore Ltd, Cork, IRL). Supernatant was loaded to a 5 ml Nickel-Sepharose HisTrap Chelating column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were placed in a humid chamber and cells were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse monoclonal anti-FLAG clone M2 (Merck F1804; 1:1000), rabbit monoclonal anti-KTN1 clone D5F7J (Cell Signaling Technology #13243 ...
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bioRxiv - Immunology 2021Quote: ... and all fractions containing IL-22 were concentrated using a 10 kDa centrifugal filter (Merck Millipore, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM PMSF was added to the second and third washes from 100 mM stock (Merck 93482). Plugs were then digested 1 h at 37°C with 1 μl 1000 U/ml RNase T1 (Thermo EN0541 ...
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bioRxiv - Systems Biology 2021Quote: ... Filter plates from NUNC (Thermo-Fischer) were used loaded with 10 mg per well of Fmoc-preloaded polystyrene resins (Merck Millipore). Fmoc-amino acids (Fmoc-AA ...
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bioRxiv - Plant Biology 2020Quote: ... and their buffer was replaced by 50 mM Tris-HCl pH 8.0 and 0.5 M NaCl by using a 10 kDa cutoff Amicon Ultra 0.5ml centrifugal filter (Merck).
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bioRxiv - Plant Biology 2020Quote: ... The digested samples were diluted to 10 mL with 18.2 MΩcm Milli-Q Direct water (Merck Millipore). Elemental analysis was performed with an inductively coupled plasma-mass spectrometry (ICP-MS) ...
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bioRxiv - Plant Biology 2020Quote: ... The digested samples were diluted to 10 mL with 18.2 MΩcm Milli-Q Direct water (Merck Millipore). Elemental analysis was performed using an ICP-MS ...
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bioRxiv - Molecular Biology 2021Quote: ... and isolated murine hepatocytes (muHEP) were seeded on rat-tail collagen (10 µg cm−1, Merck Millipore) coated well plates at a density of 0.1×106 cells per cm² ...
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bioRxiv - Biophysics 2020Quote: ... Then His-tag containing OpuAC was introduced to the flow cell (in buffer B supplemented with 10 mM of (±)6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; Merck) as a photostabilizer[20] ...
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bioRxiv - Biophysics 2021Quote: ... The dry lipid film was dehydrated with 500 μL of 10 mM HEPES (Merck Life Science, Norway) and 100 mM NaCl (pH 7.8 adjusted with NaOH ...