Labshake search
Citations for Merck :
2401 - 2450 of 5322 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Chromatin and its associated proteins were solubilized by incubation with 4- 20 units of Micrococcal nuclease (MNase; Merck Life Sciences) for 10 minutes at 37° C ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 600 ng DNA (per well of a 4-well LabTek dish) using 0.75 μl Genejuice (Merck Millipore) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: Culturing medium was changed 24 h post-transfection and vector supernatants were collected 48 h after transfection and processed by centrifugation at 1000 g for 10 mins at 4°C to remove cellular debris followed by microfiltration using Millex-HV 0.45 μm syringe filter units (Merck – SLHV033RB). Viral supernatants were either kept on ice for further use or frozen down at −80°C for storage.
-
bioRxiv - Immunology 2022Quote: ... 500ng/ml purified total IgG stimulation was added the next day for 4 hrs followed by an incubation of 30 min with ATP (Merck). Culture supernatants were collected for further cytokine analysis and cells lysates were prepared for qPCR analysis ...
-
bioRxiv - Microbiology 2022Quote: ... Coverslips were washed ten times in PBS and ten times in ultrapure water before mounting on slides using Mowiol 4–88 (Merck) containing 200 nM 4′,6-diamidino-2- phenylindole (DAPI) ...
-
bioRxiv - Molecular Biology 2022Quote: Conditioned medium was centrifuged at 3,200xg for 10 min at 4°C to remove debris and transferred to 10 kDa spin columns (Amicon Ultra, Merck). Proteins that may influence lactate and pyruvate level were removed from the <10 kDa flow-through containing metabolites by centrifugation at 14,000xg for 25 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... containing a knockout of DNA Ligase 4) [64] that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT, Merck). The transformed cells were plated on minimal synthetic defined (SD ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were centrifuged at 3041 × g for 30 minutes at 4°C and supernatant was applied to a 500 mL Stericup-HV sterile vacuum filtration system (Merck) with a pore size of 0.22 µm ...
-
bioRxiv - Microbiology 2022Quote: ... through six dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with 10 kDa molecular weight cutoff (Merck). For experiments studying the IgG-binding properties of Fc-III and Scr ...
-
bioRxiv - Cell Biology 2022Quote: ... in PBS for ten minutes at 4 °C and then washed in 0.1% Tween20 in PBS (PBST) (Merck, Darmstadt, Germany) and blocked with 5% goat sera (Merck ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... A 6:4 ratio of 0.1 % Oil-Red-O in isopropanol to 4 mL distilled water was prepared and passed through a 0.22 □m syringe filter (Merck: Z260479) before use ...
-
bioRxiv - Biochemistry 2024Quote: ... leaf punches were taken with a 4-mm biopsy punch and floated in 100 μL of H2O with 20 μM coelenterazine (Merck), using individual cells of a white 96-well white bottom plate (Greiner F-Boden ...
-
bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
-
bioRxiv - Physiology 2024Quote: ... the excess of reagents was eliminated by performing several washing steps with Milli-Q water using 4 mL cellulose membrane centrifugal filters (Amicon, MilliPore, 100 kDa, Merck). All MNP suspensions were sterilised by filtration using 0.22 μm MilliPore® filters before addition to cell cultures ...
-
bioRxiv - Cell Biology 2024Quote: ... The next day the cleaved protein was collected and concentrated by spinning the sample at 3500 RPM at 4°C using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44 ...
-
bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
-
bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
-
bioRxiv - Developmental Biology 2023Quote: Upper and lower arms were harvested from 4 cm double reporter axolotls (Alx4:mCherry_Hand2:EGFP) and dissociated into single cell suspensions using Liberase TM enzyme (Merck 05401127001) as described in 49 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10,000 cells were seeded in 12-well dishes and co-infected the next day with 4EBP1 and 4EBP2 shRNA viruses in medium supplemented with 4 µg/ml polybrene (Merck). Cells were grown until confluency and reseeded into 6-well dishes prior to addition of 1 µg/ml puromycin (InvivoGen ...
-
bioRxiv - Neuroscience 2023Quote: ... before transcardially perfusing with 21-28 ml of ice-cold PBS and 4%PFA-0,12% picric acid (Merck, Søborg, Denmark). Spleens were excised and weighed ...
-
bioRxiv - Neuroscience 2023Quote: ... heated to 37°C with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin79 ...
-
bioRxiv - Molecular Biology 2023Quote: ... equipped with a LiChrospher® 100 RP-18 (125 mm x 4 mm, 5 µm) C18 reversed-phase column (Merck). The elution solvents consisted of ultrapure water with 0.1% formic acid (A ...
-
bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin- labeled probes and biotin-labeled probes were detected with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/1,000 diluted streptavidin conjugated with DyLight 549 (Vector Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were plated at low density following incubation with DNA or siRNA 24h prior to fixation with 4% paraformaldehyde (PFA, Merck) for 15min at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: Tissues were dissected out and homogenized at 4°C in RIPA buffer containing protease inhibitors cocktail (Merck Millipore, Milano, Italy) for 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... 250 µl of undiluted chromatin was incubated overnight at 4°C with Dynabeads M-270 Epoxy preincubated with anti-Tyr1P (MABE350, Merck), anti-Ser2P (C15200005-50 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
-
bioRxiv - Plant Biology 2024Quote: ... digestion of roots was performed at 90°C during 4 h in 400 µL of 65%(w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.65% (w/v ...
-
bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
-
bioRxiv - Molecular Biology 2024Quote: Candidate genes were amplified from Drosophila melanogaster ovary cDNA library using KOD Hot Start DNA Polymerase (Merck Millipore, 71086-4) and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation (20.000xg, 15 min, 4°C) and protein concentration was determined by Direct Detect® spectrometer (Merck). The proteasome activity was measured using a 96-well plate ...
-
bioRxiv - Molecular Biology 2020Quote: ... Tubulin beta (1:8,000; clone KMX-1 from Merck Millipore), dMi-2 (1:8,000 ...
-
bioRxiv - Neuroscience 2019Quote: ... anti-vGlut (Merck Millipore, cat MAB5502, 1:200; 1:1,000); anti-GluR2 (Merck Millipore ...
-
bioRxiv - Neuroscience 2021Quote: ... and anti-TUJ-1 antibodies (1:200; Merck-Millipore, MAB380) for 48 h ...
-
bioRxiv - Microbiology 2021Quote: ... GLP-1 (GLP-1 total ELISA kit, Merck, Darmstadt, Germany) and biochemical parameters including ALT ...
-
bioRxiv - Cell Biology 2021Quote: ... Armenian hamster PECAM-1 (MAB1398Z, Merck Millipore, IHC: 1/500); goat VE-cadherin (sc-6458 ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 μL RNase A (10 U µL−1) (Merck) per mL of stock and incubated at 37°C for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 mg ml−1 collagenase IV (Merck Millipore, C4-22) and 70 U ml−1 DNase (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... 1% (vol/vol) Protease Inhibitor Cocktail Set 1 (Merck Millipore) and 1x PhosSTOP (Roche) ...
-
bioRxiv - Cell Biology 2024Quote: ... 1% (vol/vol) Protease Inhibitor Cocktail Set 1 (Merck Millipore) and 1x PhosSTOP (Roche) ...
-
bioRxiv - Cell Biology 2020Quote: ... pDEST42-Ctdnep1_C-ter plasmid was transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore), Bacteria cultures (500 ml ...
-
bioRxiv - Cell Biology 2020Quote: A549 cells were treated with 2 ng/ml TGF-β1 (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 2 days in DMEM with 10% FBS on non-treated culture dish surfaces (31 ...
-
bioRxiv - Microbiology 2019Quote: ... cleared by xylene (2 x 5 min) and finally mounted in Entellan mounting medium (Merck, Germany). HE stained sections were observed and recorded using an ECLIPSE Ni microscope (Nikon ...
-
bioRxiv - Immunology 2020Quote: ... After each cycle the flow channels were regenerated for 10 seconds with 2 M NaCl (Merck) at 10 µL/min ...
-
bioRxiv - Bioengineering 2021Quote: ... with Pellicon 2 mini cassettes (300 kDa, 0.1 m2, BioMax polyethersulfone membrane with C-screen; Merck), as previously described (4) ...
-
bioRxiv - Cancer Biology 2020Quote: ... media was replaced with media containing 10 nM 5-Bromo-2′-deoxyuridine (BrdU) (Merck, B5002-100MG). Coverslips were then fixed ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were plated at a density of 100,000cm-2 in 96 well plates (Corning, MERCK UK), with wells previously coated for 24h with 20μg/ml FHL-1 ...
-
bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37°C and purified using a Amicon 30 kDa MWCO filter (Merck). Deep Vent exo-DNA polymerase (NEB ...
-
bioRxiv - Biochemistry 2019Quote: ... 2 µl were spotted onto a thin layer chromatography (TLC) plate (PEI Cellulose F; Merck Millipore) pre-spotted with 2 µl of nucleotide mix containing AMP ...