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Citations for Merck :
2251 - 2300 of 2552 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The pellet containing the FoIV1-rORF4 inclusion bodies was obtained by centrifugation at 12,000 × g for 20 min at 4°C and then suspended in 0.1× BugBuster Protein Extraction Reagent (Merck KGaA) using a homogenizer ...
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bioRxiv - Biochemistry 2024Quote: ... Fixed cells were stained with DAPI and mounted on Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore) for imaging.
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bioRxiv - Biochemistry 2024Quote: ... containing kinase assay buffer (final concentration: 40 mM HEPES, 120 mM NaCl, [pH 7.5] and 0.3% CHAPS, 4 mM MnCl2 (Merck, 1059270100), 10 mM DTT (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 2.7 mL of each sample was transferred into an Amicon Ultra-4 3K (3000 MWCO) filtration tube (Merck Millipore) and centrifuged for 40 minutes at 6225 g ...
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bioRxiv - Plant Biology 2024Quote: ... The lumen proteins were concentrated at 14,000 rpm for 10 min at 4 °C using Amicon ultra-0.5 centrifugal filters (Cat. No. UFC500324, Merck millipore). All solutions contained protease inhibitors with final concentration of 1 mM benzamidine ...
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bioRxiv - Biochemistry 2024Quote: ... for 4 h at 4°C and then concentrated to 200 ng/μl using Amicon Ultra-0.5 centrifugal filter units (Merck). Nucleosomes are then diluted with ice-cold 0 M FRET buffer to a concentration of 50 nM ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by overnight incubation at 4°C with 250µl of the anti-DIG antibody (Merck #11093274910 diluted 1:2000) and other necessary primary antibodies (when additional immunostaining was needed ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were permeabilised with 0.5% Triton X-100/PBS for 1 hour and blocked for 1 hour with 4% donkey serum/PBS (Merck), both at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... and 10% ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Biochemistry 2022Quote: ... [2H7]22R-Hydroxycholest-4-en-3-one ([2H7]22R- HCO) was prepared from [2H7]22R-HC by treatment with cholesterol oxidase enzyme (Streptomyces sp., Merck, Dorset, UK) (11).
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bioRxiv - Microbiology 2021Quote: ... and 10 % Ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in vehicle (Ringer solution; NaCl 140 mM, CaCl21.2 mM, KCl 3 mM and MgCl2 1 mM (Merck KGaA Darmstadt, Germany)) to a dilution of 5.5μg/μl ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Physiology 2022Quote: ... and separation was carried out at 40°C using a ZIC-HILIC column (2.1 mm × 100 mm, 3 μm; Merck Sequant, Umea, Sweden). Isocratic elution was performed ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MCAD activity was measured using 3-phenylpropionyl-CoA (custom synthesis; 0.3 mM) as substrate and ferrocenium hexafluorophosphate as electron acceptor (Merck, 388297; 1.5 mM), followed by detection of 3-phenylpropenonyl-CoA using UHPLC (Wanders ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were removed from the samples by ultrafiltration at 3000 g and 30 min using Amicon Ultra filters (3 kDa cut-off, Merck, Darmstadt, Germany). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL of culture was sonicated for 3 s and 200 μL were loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Merck Millipore) connected to the CellASIC® microfluidic pump (Merck Millipore) ...
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bioRxiv - Biophysics 2021Quote: ... 250 ng of each plasmid encoding VN and VC were transfected using 4 μL of 1 μg/mL PEI (Merck) for each μg of DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 cells from treated and untreated conditions were centrifuged for 5 min at 4,500 g and the pellets were resuspended in 4% paraformaldehyde (PFA, Merck, Germany), and incubated for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Microbiology 2021Quote: ... containing a knockout of DNA Ligase 4) 44 that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT; Merck). The transformed cells were plated on minimal synthetic defined (SD ...
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bioRxiv - Biophysics 2020Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with 10kDa Amicon®Ultra-4 centrifugal filters (MERCK). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... The resulting 5.6-mL fraction was concentrated to 250 μL by ultrafiltration using Amicon Ultra-4 10K centrifugal filters (Merck, USA), then submitted to immunodetection.
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bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in PBS/ 0,05%Tween20 containing 5% skim milk and then probed with the following primary antibodies over night at 4°C: mouse anti-P53 (1:100, Merck), rabbit anti-H2AX (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further separated by size exclusion chromatography (HiLoad 26/600 Superdex 75, Cytiva) and concentrated using Ultra-4 or 15 mL centrifugal filter devices (Amicon, Merck). Correct size and high purity were verified via SDS-PAGE and LC-MS analysis ...