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Citations for Merck :
2201 - 2250 of 2518 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and the bound IgG was eluted using Ab Buffer Kit (Merck), and then the eluate was dialyzed overnight at 4°C in PBS using a 10 kDa dialysis membrane (Thermo Fisher Scientific) ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Soluble proteins were then dosed using the Bicinchoninic Acid Kit (Merck) and diluted in Laemmli protein sample buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (Merck S7165) was used ...
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bioRxiv - Cell Biology 2022Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
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bioRxiv - Genomics 2023Quote: ... The Sigma Spectrum plant total RNA kit (Merck KGaA, Darmstadt, Germany) was used for RNA extraction that followed the manufacturer procedure ...
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bioRxiv - Genetics 2023Quote: ... using the Spectrum Plant Total RNA kit (Merck KGaA, Darmstadt, Germany). Gene expression was determined by RT-qPCR as previously described (Zenoni et al. ...
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bioRxiv - Physiology 2023Quote: ... glucose levels were determined using a Glucose (GO) assay kit (Merck). Blood insulin concentrations were measured by Rat Insulin ELISA kit (Mercodia) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated using the Spectrum Total RNA kit (Merck), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... H&E staining was carried out with a kit (Merck, Germany)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were stained with Phosphatase Alkaline staining kit (Merck-Millipore, SCR004) according to the manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: ... the QCM Chemotaxis 5µm 96-Well Cell Migration Assay Kit (Merck, Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Luminex MILLIPLEX TGF-beta 1 Single Plex MAGNETIC Bead Kit (Merck) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were immersed in Neo-Clear® three times for 5 minutes and then mounted using Neo-mount® mounting medium (Merck Millipore), coverslipped and left to dry overnight in at 42°C.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was passed through the cartridges (pre-conditioned with 5 mL each of tert-methyl butyl ether, methanol (Merck; laboratory grade purity) and deionised water ...
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bioRxiv - Microbiology 2022Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Systems Biology 2022Quote: ... Chromatographic separation was performed on either of the three columns: a ZIC-pHILIC column (Merck, 150 mm × 2.1 mm, 5 μm particle size), a reverse-phase C18 Kinetex Evo column (Phenomenex ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μl of cell suspension was collected and mixed with 200 μl of ice-cold HBSS containing 5% fatty acid-free bovine serum albumin (BSA, Merck Millipore, 126575-10GM). To quench the fluorescence of non-internalized NBD-phospholipids ...
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Physiology 2023Quote: ... Pigment separation was performed by reverse-phase HPLC as described in (Färber et al., 1997) using a Dionex BioLC HPLC system (Sunnyvale, California, US) with LiChrospher® 100 RP-18 (5 µm) column (Merck, Darmstadt, Germany). Xanthophylls ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Genetics 2022Quote: ... 5 µg SA8 was dissolved in 10 µL 10 mM hydrochloric acid and digested with 20 ng/µL pepsin (Merck, cat. No. 10108057001) at 37 °C for 6 h ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed on the top of a water-cooled glass column (33 × 2.5 cm) filled with a slurry of silica gel 60 (with the addition of 7 % water, 40 – 63 μm, Merck, Darmstadt, Germany, # 1.09385.2500) and n-pentane ...
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bioRxiv - Molecular Biology 2023Quote: ... Milli-Q water was used for preparing all aqueous solutions (18 MΩ×cm, <5 ppb, Millipore Milli-Q® Reference A+ system, Merck Millipore, Germany). LC-MS grade acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: Seed cultures for dual species biofilm were generated from single colonies of each species inoculated as monocultures in 5 ml of Lysogeny Broth (Merck Life Science, UK) supplemented with 0.2% glucose ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
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bioRxiv - Microbiology 2024Quote: ... column (Waters, Milford, MA, USA), a SeQuant ZIC-pHILIC PEEK-coated (100×4.6 mm, 5 µm, PEEK coated) column (Merck Millipore, Burlington, MA, USA), or a SeQuant ZIC-cHILIC (100×4.6mm ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM TCEP) and concentrated to approximately 5 ml using an Amicon® Ultra Centrifugal Filter Ultracel®-3K (UFC900324, Merck/Millipore, Darmstadt, Germany) before loading onto a HiTrap™-Heparin HP column (Cytiva ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Plant Biology 2022Quote: ... Spore concentration was counted via haemocytometer and adjusted to 1 x 105 spores per mL in 0.1% gelatin or dH2O with 0.01% Tween 20 (Merck Chemicals, CAS Number: 9005-64-5).
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bioRxiv - Pharmacology and Toxicology 2024Quote: HPLC separations were performed on a Waters 2695 separations module equipped with a Waters 2996 photodiode array detector and HPLC analysis was carried out using a LiChrospher® 100 RP-18 column (4 mm × 250 mm, 5 μm) (Merck, Kenilworth, NJ, USA). The mobile phase consisted of two solvent reservoirs ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4° C ...
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bioRxiv - Cell Biology 2020Quote: ... ChIP assays were performed using a specific kit (Merck Millipore, Darmstadt, Germany). After crosslinking ...
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bioRxiv - Cell Biology 2020Quote: PP2A activity were measured by PP2A phosphatase activity assay kit from Merck Millipore (PP2A immunoprecipitation phosphatase assay kit ...
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bioRxiv - Microbiology 2021Quote: ... and a solution of XTT/PMS (Cell Proliferation Kit II; Merck, Germany) and culture medium was added to the wells ...
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bioRxiv - Cancer Biology 2020Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (S7165, Merck Millipore) was also used to label apoptotic cells following Wells and Johnston protocol (Wells and Johnston ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using a Magna Nuclear RIP Kit from Merck Millipore (17-10520 ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Molecular Biology 2022Quote: PLA detection was performed using a Duolink In Situ Orange Kit (Merck) according to the manufacturer’s protocol ...