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Citations for Merck :
2201 - 2250 of 2709 citations for Mouse Anti Chikungunya Virus E2 Protein 18H01 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers, MA, USA; sheep anti-αMSH, 1:1000, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots were retained for direct Western blot analysis (Input) and otherwise subjected to immunoprecipitation (IP) with protein A-coupled Sepharose beads (Merck, 17-5280-02) pre-incubated with 2 μg anti-RbBP5 antibody or IgG at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Measurements were run in triplicates diluting the protein sample at 50 nM concentration in NMR buffer loaded on a gasket (Grace Bio-Labs reusable CultureWellTM gaskets, Merck (#GBL103250)) mounted on a glass microscope coverslip (Marienfeld (#0107222)) ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2022Quote: Equal amounts of total protein (50 μg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of total protein (50 µg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Biophysics 2022Quote: ... equal amounts of GFP and SNAP-tagged recombinant WT-FUS protein (yielding a total protein concentration of 6 μM) were gently mixed into an aqueous solution of 20 % PEG-35 (Merck KGaA, Darmstadt, Germany) and 500 mM KCl (Merck KGaA ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Immunology 2023Quote: ... The eluted proteins were buffer exchanged into PBS and then concentrated using Amicon® 30 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9030).
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were concentrated using an AmiconTm Ultra (molecular weight cut-off 30 kDa and 50 kDa) centrifugal filter device (Merck Millipore, Darmstadt, Germany). Fractions from all purification steps were collected and analyzed by SDS- PAGE.
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bioRxiv - Molecular Biology 2020Quote: ... 600 µg WCL was mixed with anti-Flag antibody beads (Merck) at 4°C for 3 hr with rotation ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained for vinculin using anti-vinculin antibody (Merck Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... and rabbit anti-phospho-histone H3 antibodies (Merck Millipore, 06-570), respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... The hybridized probes were detected with anti-Dig AP (Merck, #11093274910) and CDP-Star (Merck ...
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bioRxiv - Systems Biology 2021Quote: ... The following antibodies and concentrations were used: anti-GAPDH (Merck #MAB374) 1/5,000 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti phospho-ubiquitin (Ser65) (Merck Millipore, ABS1513-I, 1:1000), mouse anti GAPDH (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... and rabbit anti-peripherin (1/500, Merck Millipore, AB1530, RRID: AB_90725). The analyses of L1-L5 and C4-C8 wild-type DRG were performed at different times ...
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bioRxiv - Plant Biology 2021Quote: ... and mixed with 20 μl anti-FLAG magnetic beads (Merck Millipore). Following 1 hr ...
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bioRxiv - Plant Biology 2021Quote: ... and mixed with 20 μL anti-MyC magnetic beads (Merck Millipore). Following 1 hr incubation at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-GluN2A monoclonal antibody (1:1000; Merck KGaA, Darmstadt, Germany). After three washes in TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... The antibody aggregates consisted of goat anti-biotin (#B3640; Merck, Germany) and STAR635P-conjugated donkey anti-goat (#ST635P ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-OC against high molecular weight Aβ (Merck Millipore, Sweden), mouse anti-6E10 for human Aβ/APP (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... we used a goat anti-ChAT (1/100; Merck-Millipore, France) or a mouse anti-islet 1,2 (1/250 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then stained with DAPI anti-fade mounting media (Merck). For 1,6-Hexanediol (HD ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were: rabbit anti-TH (1:1000, Merck Millipore, #AB152), anti-mouse anti-Cre (1:500 ab24607) ...
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bioRxiv - Developmental Biology 2022Quote: ... The western blot was performed using anti-puromycin (1:1000, Merck) and anti-mouse-horseradish peroxidase (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-phospho-Histone H2A.X Ser139 (γH2AX, clone JBW301, Merck Millipore, USA) or anti-53BP1 antibody (H-300 ...
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bioRxiv - Immunology 2020Quote: ... were coated respectively with anti-AKT (clone SKB1, Merck Group, Germany) or anti-PTPN22 (clone D6D1H ...
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bioRxiv - Neuroscience 2019Quote: ... at 1:500 or polyclonal rabbit anti-Prox1 antibody (Millipore; Merck KGaA ...
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bioRxiv - Physiology 2019Quote: ... Antibodies used were rabbit anti-MCT4 (1:250; AB3314P, Merck Millipore), mouse anti- β-actin (1:2,000 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Tau-1 antibody (non-phosphorylated Tau, clone PC1C6, MERCK, MAB3420), Tau monoclonal antibody (TAU-5 ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal anti-phospho CREB1 conjugate (06-519-AF647, Merck, Germany) and rabbit polyclonal activating transcription factor-4 (ATF4 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant rabbit anti-pan-ADP-ribose binding reagent MABE1016 (Merck Millipore) was used 1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... and α-MAR/PAR (anti-pan-ADP-ribose binding reagent, Merck). Proteins were detected using HRP-coupled secondary antibodies and X-ray films.
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-COLLAGEN type IV (cat. # AB756P, Merck Millipore, 1:500), mouse anti-Neurofilament (cat ...