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Citations for Merck :
2201 - 2250 of 5475 citations for 7H 1 3 Thiazino 2 3 i purin 5 1H one 2 3 8 9 tetrahydro 2 thioxo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Protein content of supernatant samples were normalized and analyzed using SDS-PAGE (mPAGE Bis-Tris 8%, Merck, Darmstadt, Germany) revealing specific protein bands in the supernatant while comparing wildtype and the T2SS mutant (xpsxcs ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... immunoprecipitations performed by incubating with 8 μl Protein G Dynabeads (Novex) and 12 μl anti-FLAG antibody (F3165, Merck) per sample for 90 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... was eluted with solution-I and was concentrated to with an Amicon Ultra 10-kDa MWCO concentrator (Merck Millipore). The protein was further subjected to size exclusion chromatography on a HiLoad 16/600 Superdex 75 column pg column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... the dermis was cut and put in 3.5 ml of Dermis Dissociation Buffer composed of 100 μg/mL of DNASE I (Merck) and 1 mg/ml collagenase P (Merck ...
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bioRxiv - Microbiology 2021Quote: ... The diced tissue was then digested for 60 minutes at 37°C in 12ml of a pre-heated suspension of collagenase I (2.4mg/ml, Merck) and DNase I (1mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we re-analyzed microarray-based transcriptome datasets: i) HepG2 cells treated with vehicle (0.0125% DMSO) or 12 μg/ml silymarin (Merck) for 24 h (n = 3 biological replicates)98 ...
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bioRxiv - Plant Biology 2022Quote: ... Spore concentration was counted via haemocytometer and adjusted to 1 x 105 spores per mL in 0.1% gelatin or dH2O with 0.01% Tween 20 (Merck Chemicals, CAS Number: 9005-64-5).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and host plants was assessed by extracting the compounds following [6] by immersing the samples for 5 min in 5 ml of hexane (99%, SupraSolv, Merck, Germany), followed by removal from hexane with entomological tweezers that were previously cleaned with hexane ...
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bioRxiv - Molecular Biology 2019Quote: To evaluate microscopic features of the captured cells such as morphological types and possible alterations we then used another collection membrane directly stained with hematoxylin and eosin after fixation for 10 min in a fixation solution (100 mL of 70% ethanol, 5 mL of glacial acetic acid and 5 mL of 37% formaldehyde solution – all solutions are from Merck Millipore). Samples were hydrated with distilled water for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... the MFI-value was converted to ng/mL by interpolation from a 5-parameter logistic (5-PL) curve of reference standard using the MILLIPLEX® Analyst 5.1 software (The Life Science/Merck KGaA).
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bioRxiv - Cancer Biology 2024Quote: ... and non-specific binding was blocked with 5% milk powder or 5% bovine serum albumin (BSA) in tris-buffered saline containing 0.05% Tween-20 (Merck, Darmstadt, Germany). Blots were incubated overnight with mouse anti-gp130 (R&D systems ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μM of para-amino-Blebbistatin (Optopharma, DR-Am-89) was applied for one hour to inhibit NMII activity and 100 μM CK-666 (Merck, SML0006) for four hours to inhibit the Arp2/3 complex ...
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bioRxiv - Immunology 2021Quote: ... 20 μl of this cell/collagen mixture was added in one well of pre-cooled Corning™ 96-Well half-area plate (Merck) and the plate was centrifuged ...
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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bioRxiv - Microbiology 2024Quote: ... All but one sample for microbiological analyses was collected onto 0.22 μm pore size filters (type GVWP; Merck Millipore, Darmstadt, Germany) held in pressure-resistant stainless steel filter holders directly connected to the tubing outlet under in-situ hydraulic pressure conditions ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown in 25% TSB medium at 28°C in static conditions in glass microcosms with one layer of ColiRoller glass beads (Millipore, Merck, USA) covering the base of the microcosm to provide additional spatial structure ...
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bioRxiv - Bioengineering 2022Quote: ... at a 0.2 μM synthesis scale with desalting purification (HPLC purification in case of labelled ones) were purchased from Merck (Sigma-Aldrich). Mowiol® 4-88 ...
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bioRxiv - Biophysics 2021Quote: ... The His-tag cleaved Mpro in the flow-through was buffer-exchanged to 20mM Tris pH 8 using Amicon Ultra centrifugal filter (MWCO. 10kDa, Merck) and then loaded onto 5mL Q Sepharose column (HiTrap Q HP ...
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bioRxiv - Molecular Biology 2021Quote: ... Smchd1 deletion was confirmed by immunofluorescence with an in-house made anti-Smchd1 antibody during the immunofluorescence experiments (Mab #8, now available commercially from Merck).
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bioRxiv - Pathology 2020Quote: ... was performed placing the sections in a 800 ml glass container filled with the retrieval solutions (10 mM EDTA in Tris-buffer pH 8, Merck), irradiate in a household microwave oven at full speed for 8 min ...
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bioRxiv - Biochemistry 2020Quote: Protein pellets were dissolved in 50 μl of 8 M Urea and loaded onto a 30 K centrifugal filter unit (Merck). Preparation for MS was done as described in [64] ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were transferred into 50 mL conical tubes with 20 mL of SM buffer (50 mM Tris HCl, 100 mM NaCl, 8 mM MgSO4, pH 7.5, Merck, Germany) and vortexed for 60 s to remove phage Φ6 particles attached to sample surfaces ...
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bioRxiv - Cell Biology 2020Quote: ... MiRNA in situ hybridization was performed in formaldehyde and carbodiimide (EDC)-fixed TA cryo-sections (0,16M 90 min at RT, #25952-53-8, Merck KGaA). After washes with 0,2% glycine (#G8898 ...
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bioRxiv - Microbiology 2022Quote: ... 1.5×104 conidia of each strain were inoculated in 300 µL of RPMI in the presence of 8 µg/mL voriconazole (European Pharmacopoeia, -EP- Reference Standard, Merck) and incubated for 48 hours at 37 °C with occasional shaking ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were then lysed in lysis buffer (50 mM KHPO4 pH 8, 100 mM NaCl, 10 mM Imidazole, 1X BugBuster (Merck), 25 U/ml Benzonase nuclease ...
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bioRxiv - Cancer Biology 2020Quote: ... Gaithersburg, MD, USA) and 8-μm-pore polycarbonate membranes (Nucleopore, Pleasanton, CA, USA) coated with 10 μg/ml fibronectin (Merck) as described elsewhere (Prevete et al. ...
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bioRxiv - Neuroscience 2022Quote: ... cells were replated onto coverglass (8×104 per well) coated with 50 µg/ml Poly-D-Lysine (PDL, Merck, USA) for imaging experiments.
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bioRxiv - Molecular Biology 2022Quote: ... LV tissue was solubilized in 50 mM Tris-SDS buffer (pH 6.8) containing 8 µg/mL leupeptin (Merck Life Science) and phosphatase inhibitor cocktail (Merck Life Science) ...
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bioRxiv - Molecular Biology 2023Quote: ... then the mixture was dialyzed against histone refolding buffer at 4 □ using a dialysis tube (D-TubeTM Dialyzer Medi or Maxi, MWCO 6-8 kDa, Merck). HE buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Forty eight hours of post-transfection the viral supernatants were passed through 0.45 μm syringe and the diluted viruses were used to infect cells in growth media supplement with 8 μg/ml of fresh polybrene (Merck) for 8 hr for three consecutive times ...
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bioRxiv - Cell Biology 2023Quote: For the thin layer chromatography (TCL) an aliquot of lipid extract corresponding to 8 mg of DCW was applied to silica gel TLC plates (Merck) by a Linomat 5 semiautomatic sample applicator (Camag) ...
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bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Clones were trial-screened for positive PCR products by making mirror plates and subjecting one of the plates to cell lysis (50mM Tris-HCl pH 8, 1mM EDTA, 0.5% Tween-20, 50-80 μg/ml Proteinase K Merck-3115801001) at 37°C overnight ...
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bioRxiv - Immunology 2023Quote: ... WT mice were 7-8 week-old Mice (C57BL/6 WT or Nr4a3-Timer-GS) were injected with 10 mg/kg azoxymethane (Merck) via i.p ...
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bioRxiv - Cell Biology 2024Quote: Trophozoites of the transformant strain expressing HA-EhRacM and HA- EhRacJ were suspended with BIS medium and transferred to a Millicell EZ SLIDE 8 well glass slide (Merck) to culture ...
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bioRxiv - Neuroscience 2024Quote: ... Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck) and 500 mM sodium chloride ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-20 µg protein lysate were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) using 8-10% gels and transferred to Amersham Protran 0.45 µm nitrocellulose membranes (Merck, #GE10600012) by wet blotting using the Mini-Protean Vertical Electrophoresis system (Bio- Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore ...
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bioRxiv - Immunology 2024Quote: ... Viral supernatant was filtered and used for transduction of NALM6 and K562 cells through spinoculation together with 8 μg/ml protamine sulfate (Merck). Transduced cells were selected using puromycin (1 μg/ml).
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bioRxiv - Synthetic Biology 2024Quote: ... Stimulation of the controls was performed 8 h after transfection with 20 ng/ml TNF-α (Merck, cat. no.: H8916). Cells were lysed 24 h later by addition of 100 μl lysis buffer (25 mM Tris/HCl ...
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bioRxiv - Biochemistry 2019Quote: ... resuspended in lysis buffer (50 mM sodium phosphate pH 7.5, 500 mM NaCl, lysozyme, Dnase I and cOmplete™ Protease Inhibitor Cocktail [Merck]), and disrupted by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue was dissociated for 30-40 min using 20 U/mL pre-activated papain (Bionordica, WBT-LS003126) and washed in DMEM containing 0.01 mg/mL DNase I (Merck, DN25) and 10% FBS before being triturated in the same buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck, DNASE70). cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD ...
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bioRxiv - Evolutionary Biology 2023Quote: ... starting with a digestion of animal tissues form different life-stages in 500ml of Animal Free Collagenase/Dispase Blend I (Merck) dissolved in 35‰ FSASW (filter sterile artificial sea water ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 µL of the washed GUV solution was transferred to an 18-well imaging chamber and supplemented with 10 mM Magnesium-Ionophor I (Merck). Once the sample was placed on the confocal microscope with an incubation chamber held at 37 ◦C for 2 h ...