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Citations for Merck :
2101 - 2150 of 2799 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Surface antigens were labeled with primary antibody (rabbit anti-GFP, 1:1000, Merck) for 15 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Nonspecific antibody blocking was for 1 h with 2% bovine serum albumin (Merck) in Tris-buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with a 1:10,000 dilution of primary antibody MABE1016 (Merck) in 10 ml washing buffer (1 % [w/v] milk powder in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... 1µl NeuN antibody (1:500, Millimark mouse anti-NeuN PE conjugated, FCMAB317PE, Merck) was added and samples were incubated for 30 min on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies used for immunoblotting were: α-FLAG M2 (1:500; Merck-Sigma, F3165), α-Matr3 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... a rabbit anti-H3R26me2 antibody (1:8000 dilution; 07-215; Merck, Darmstadt, Germany), a mouse anti–β-actin antibody (1:5000 dilution ...
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bioRxiv - Cell Biology 2023Quote: The anti-Flag primary antibody was purchased from Merck (Darmstadt, Germany: Flag F3165). Secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used: anti-CD2AP (Merck, HPA00326, 1:100 (IF); 1:1000 (WB)) ...
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bioRxiv - Genetics 2023Quote: ... α-tubulin antibody (used with 1:5000 dilution) was purchased from Merck (#T5168). Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... and the remaining supernatants then added to 4µg rabbit anti-HA antibody (Merck). After 30 minutes incubation at 4⁰C on a rotator ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Primary antibodies used in the Immunofluorescence are mouse anti-PCNA (1:200, Merck) and mouse anti-HuC (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies and dilutions were used: Amylase (1:400, Merck A8273), cleaved-Casp3 (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used were anti-rabbit IgG (Fc) TRITC (Merck, # SAB3700846, 1:500) and anti-mouse IgG (Fc ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used with this protocol were the following: H3.3 (09-838 – Merck-Millipore); H3K9me3 (D4W1U – Cell Signalling Technology) ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate was incubated with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Centrin-1 20H5 antibody (Merck Millipore#04-1624, 1:200 IF), mouse anti-α tubulin antibody (Sigma#T9026 ...
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bioRxiv - Plant Biology 2024Quote: ... the blots were incubated with primary antibodies α-FLAG (Merck, F3165, 1:2000), α-GFP (Chromotek ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used: Glutamine Synthetase (monoclonal mouse, MAB302, Merck Millipore, USA), NeuN (monoclonal mouse ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate underwent immunoprecipitation with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: ... Sonicated DNA was used for immunoprecipitation with BG4 antibody (anti-GQS, Merck Millipore) bound on anti-FLAG dyna beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... antibodies were purified using the Amicon Ultra-0.5 Centrifugal Filter Units (Merck, UFC510024) as follows ...
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bioRxiv - Cell Biology 2024Quote: ... using the following primary antibodies: mouse anti-RAD2l (0S-908, Merck Millipore, l:S00), rabbit anti-SMC2 (abl04l2 ...
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bioRxiv - Immunology 2024Quote: ... The membranes were incubated overnight at 4 °C with detecting antibodies (Merck, #F1804). Afterward ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used an antibody targeting TH (Tyrosine Hydroxylase, AB152, Merck Millipore, MA, USA) which synthesizes dopamine ...
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bioRxiv - Microbiology 2024Quote: ... followed by goat anti-rabbit IgG horseradish peroxidase-conjugated antibodies (1:20000) (Merck), and in between membranes were washed in TTBS ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies were used: antiserum against NeuN (1:1,000; MAB377, Merck); antiserum against TDP-43 N-terminus (1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Genetics 2021Quote: ... For western blotting the proteins were resolved in SDS-polyacrylamide gels and transferred electrophoretically at room temperature to PVDF membranes (Merck) for 1 h at 50 mA using a Tris-glycine buffer system ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... and monomeric protein was purified by size-exclusion chromatography using a HiLoad™ 26/600 Superdex™ 75 pg (Merck) equilibrated with 25 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... lyophilized protein was dissolved in sodium phosphate buffer (pH 7.4) and filtered using 0.22 μm low protein binding membranes (Millex-GP, Merck Millipore, Germany). The protein concentration was determined by the measurement of absorbance at 277 nm using the extinction coefficient 5960 cm-1M-1.
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bioRxiv - Molecular Biology 2019Quote: ... RIP-Seq experiments with UV cross-linking were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700 Merck). Briefly ...
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were digested by addition of porcine trypsin (enzyme to protein ratio of 1:100; for 2 x104 microglial samples, 300 ng was used; Merck) and incubated overnight at 37 °C with shaking ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchange to PBS and the concentration of the protein were done by using amicons® (Merck Millipore Ltd, cat:UFC900324).
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... the pure huPrP protein (i.e., with the HisTag removed) was concentrated using an Amicon Ultra 0.5 ml centrifugal filter (Merck & Co., USA) with an NMWL cutoff of 3 kDa ...
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bioRxiv - Biophysics 2021Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated using amicon 10 k MWCO centrifugal filtration devices (Merck). Protein purity was determined by aRP to be 88.6% (Supplementary Figure 8) ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using Millex Non-Sterile Low Protein Binding Hydrophilic LCR (PTFE) Membrane (0.45 µm) filters (Merck #SLLHR04NL) into 1.5 mL MS vials with silicone/PTFE septa and stored at -20 °C for later analysis.
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Biophysics 2019Quote: ... Protein contaminants were eliminated by subjecting the combined IF1 fractions to Amicon® Ultra 30K Da centrifugal filters (Merck, Germany) followed by concentration on a HiTrap™ SP HP (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2019Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated by using amicon 10 k MWCO centrifugal filtration devices (Merck).