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Citations for Merck :
2101 - 2150 of 2313 citations for 5 TERT BUTOXYCARBONYLAMINO 2 CHLORO NICOTINIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Nuclei suspension was filtered, centrifuged and resuspended in RNAse-free PBS (AM9624, ThermoFischer Scientific) with 2% Bovine Serum Albumin (A2153, Merck) and 0.2 U/µL RNAse inhibitor (3335399001 ...
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bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were resuspend in neutralized bovine collagen I (2 mg/ml) and plated in a black 96-well plate with flat clear bottom (Corning/Merck) at a density of 25,000 cells/40 µl per well ...
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bioRxiv - Immunology 2024Quote: The viabilities of the PBMCs or U-937 macrophages were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Plant Biology 2024Quote: ... Ultrapure water (< 2 ppb TOC) was produced using a Milli-Q Advantage A10 water purification system (Merck, Burlington, MA, USA). For mass calibration ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissociated follicles were kept on the Transwell-COL membranes contacted with IVG medium composed of αMEM supplemented with 2% polyvinylpyrrolidone (PVP360, Merck), 5% FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Microbiology 2023Quote: ... were pre-prepared by washing 3 times in immunoprecipitation buffer then resuspended in 200μl immunoprecipitation buffer and coated with 2 μl FLAG M2 (Merck Millipore) or IgG (Merck Millipore ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of gDNA from individual GCs (2 ng input) was performed with1 ul of JH reverse primer (10 uM, provided by MERCK) and 1.8 ul of LD forward primer set pools (10 uM per primer ...
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bioRxiv - Pathology 2023Quote: ... To prevent unspecific labelling the sections were incubated for 2 × 10 minutes in PB solution containing 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Biochemistry 2023Quote: 500 ng – 5 µg of pre-purified rRNA subunits (20 µL of each) were digested to single nucleosides by using 2 U benzonase nuclease (> 90 % purity, Merck) 0.2 U phosphodiesterase I (VWR) ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
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bioRxiv - Molecular Biology 2023Quote: ... Target cells without effectors served as a negative control and target cells incubated with 2% Triton X-100 (Merck-Sigma) served as positive control (maximum killing).
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Genomics 2023Quote: ... 4 % DDT and 2 % clothianidin-impregnated papers prepared in acetone and Dow Corning 556 Silicone Fluid (BHD/Merck, Hesse, Germany). Flies overexpressing CYP6Z2 were also exposed to the primary product of hydrolysis of pyrethroids ...
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bioRxiv - Biophysics 2023Quote: ... The C378A or C406A variants of 15N-pm-β2AR-Cter were labeled on the remaining cysteine using 3-(2-Iodoacetamido)-proxyl (Merck). Paramagnetic samples were recorded with a recycling delay of 2 s ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were collected and concentrated to ∼2 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All purification steps were performed at 4 °C.
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bioRxiv - Developmental Biology 2023Quote: ... 2-cell stage embryos were similarly recovered from hybrid superovulated females (using 5IU of PMSG and 5IU of hCG; Merck), generated after crossing BDF1 male and CD1 females (each strain obtained from Anlab ...
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bioRxiv - Cell Biology 2023Quote: ... the media of the cells was replaced with XF base media supplemented with 2 mM L-Glutamine (Merck Life science), 17.5 mM glucose (Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were concentrated to 2 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 300-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Neuroscience 2023Quote: ... A 0.1 M phosphate buffer at pH 7.2 was used and a 2% agarose gel enriched with monodisperse glass spheres (Monospher 100, Merck, Darmstadt) to simulate microscopic B0 inhomogeneities was added ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Genomics 2023Quote: ... DNA was fragmented in a 2 mL low-bind round bottom Eppendorf tube using a sterile 3 mm borosilicate bead (Z143928-1EA Merck) by vortexing for 1 minute at maximum speed as described in [23] ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T/phoenix-AMPHO cells were selected for stable expression of retroviral vectors using Hygromycin B (200 µg/mL) and Diphtheria toxin (2 µg/mL; Merck) [16] ...
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bioRxiv - Physiology 2023Quote: ... was used to slowly inject 200 μL of the injection mix composed of ∼2×1011 genome copies of AAV diluted in phosphate-buffered saline (P4417, Merck) containing 2.5 mM MgCl2 and 1 mM KCl ...
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bioRxiv - Systems Biology 2024Quote: ... with 100 µl of a 2 % w/v solution of Evans blue tracer in 0.9 % saline (Merck Ltd, Poole, UK) or i.v ...
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bioRxiv - Neuroscience 2024Quote: ... or (2) collected 3 days after transfection and enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Viral pellets were resuspended in sterile PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87 ...
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bioRxiv - Microbiology 2024Quote: ... The resulting concentrated retentate was then filtered through a 0.22 μm filter and added with stabilizing buffer containing 2% (v/v) gelatin hydrolysate (Merck G0262) and 5% (v/v ...
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bioRxiv - Physiology 2024Quote: ... Nuclei suspension was filtered, centrifuged and resuspended in RNAse-free PBS (AM9624, ThermoFischer Scientific) with 2% Bovine Serum Albumin (A2153, Merck) and 0.2 U/µL RNAse inhibitor (3335399001 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... About 2 mL of supernatants were then transferred to ultrafiltration tubes (Amicon® Ultra-4 Centrifugal Filter Unit 10K, Merck). 2 mL of TE buffer was added ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were recovered by withdrawing a volume of 200 μL from the 30 – 40% interface of the OptiPrep density gradient and transferring to a new 2 mL Sorenson Dolphin microcentrifuge tube (Merck). Nuclei were washed with 2% BSA (in 1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The embryos were then incubated with anti-cleaved caspase 3 pAb (1:100) (Anti-caspase-3, cleaved (Ab-2) Rabbit pAB (PC679; Merck) followed by a wash and a second incubation with anti-rabbit 568 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM Tris (2-carboxyethyl) phosphine (TCEP)] in presence of equal molar amount of Mu217 DNA in a Pur-A-Lyzer Mini (Merck). Mu217 DNA was purified as previously described7 ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were immersed in Neo-Clear® three times for 5 minutes and then mounted using Neo-mount® mounting medium (Merck Millipore), coverslipped and left to dry overnight in at 42°C.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Systems Biology 2022Quote: ... Chromatographic separation was performed on either of the three columns: a ZIC-pHILIC column (Merck, 150 mm × 2.1 mm, 5 μm particle size), a reverse-phase C18 Kinetex Evo column (Phenomenex ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Physiology 2023Quote: ... Pigment separation was performed by reverse-phase HPLC as described in (Färber et al., 1997) using a Dionex BioLC HPLC system (Sunnyvale, California, US) with LiChrospher® 100 RP-18 (5 µm) column (Merck, Darmstadt, Germany). Xanthophylls ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...