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Citations for Merck :
2051 - 2093 of 2093 citations for 7 Chloro 8 methyl 2 trifluoromethyl quinolin 4 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The culture was grown for 2 hr at 37°C and stocks were made from the original plate by adding glycerol (Merck & Co., USA) to the final concentration of 15% and stored at -70°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
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bioRxiv - Neuroscience 2023Quote: Blood was collected in 2 ml EDTA tubes that were prepared with 100µl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany). These tubes were centrifuged immediately after venepuncture for 15 min at 4⁰C and 3200g ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with 50 µM inhibitor (dissolved at 50 mM concentration in 100% DMSO) or 2 mM ATPψS (Merck Life Science UK) at room temperature for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Biochemistry 2023Quote: ... samples were concentrated to a volume of approximately 2 mL using Amicon® Ultra centrifugal filters with a NMWC of 10 kDa (cat# UFC801024, Merck Millipore).
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bioRxiv - Genetics 2023Quote: ... after adding 2 g activated (i.e., baked at 300°C for 2h) molecular sieves (5Å, 45-60 mesh size, Merck, KGaA, Darmstadt, Germany). The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Neuroscience 2022Quote: ... freezed 15 min at −80°C and thawed for 15 min at room temperature following overnight incubation in 2% TritonX-100 (Merck Millipore, 1086031000) PBS for permeabilization ...
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bioRxiv - Biophysics 2023Quote: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) stored in chloroform were purchased from Merck (Darmstadt, Germany) and stored under argon ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Neuroscience 2024Quote: ... Semithin sections (1–2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 2 h at room temperature with the following affinity pure primary antibodies: rabbit polyclonal anti-TRPM4 (Cat.# ABN418; Merck Millipore, Darmstadt, Germany) and mouse monoclonal anti-Aquaporin 4 antibody [4/18] (ab9512 ...
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bioRxiv - Microbiology 2019Quote: ... 25 µl of the extract was spotted (2 µl/transfer) onto a reverse-phase thin layer chromatography silica gel 60 RP-18 sheet (Merck, Kenilworth, NJ, USA). In addition to the LC510 AHL extract ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Microbiology 2022Quote: The His6-eVP40-pET46 expression vector was a kind gift from Erica Ollmann Saphire (La Jolla Institute for Immunology) and was expressed and grown in RosettaTM 2 BL21 DE3 competent cells (Merck Millipore, Billerica MA). The pet28a-His6-Lact C2 bacterial expression plasmid was a kind gift from Dr ...
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Microbiology 2022Quote: ... were sequentially filtered by vacuum through hydrophilic polycarbonate filters with a pore size of 20 µm (47 mm; Sterlitech, Kent, WA, US) and then 2 µm (Isopore, 47 mm; Merck Millipore, Cork, Ireland). Filters were immediately plunged into liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Cell Biology 2019Quote: ... The percentage of COX-2 positive cells was quantified on 5.103 dissociated UC-hMSCs using a Guava® easyCyte Flow Cytometer (Merck Millipore, Guyancourt, France). The results were compared to the fluorescent intensity distribution obtained by image analysis.
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bioRxiv - Microbiology 2019Quote: ... TEER was evaluated with the Millicell ERS-2 Voltohmmeter at the beginning and at the end of the assay (Merck-Millipore, Overijse, Belgium).
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Microbiology 2022Quote: ... medium was added to the apical side 30min prior to measurement with a chopstick electrode connected to a Volt-Ohm-meter (Millicell® ERS-2, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... injections 3x a week (consecutive days) during 2 weeks of 25 mg/kg 3PO or 75 mg/kg 3PO or dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Merck, Overijse, Belgium) as control ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...
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bioRxiv - Microbiology 2022Quote: ... we used the same approaches as described above and spotted the 5 μl of bacteria on LB agar plates containing 2% of Skim Milk Powder for microbiology (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany). Plates were incubated at 25°C and monitored for hemolytic and protease activities after 1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured as described in 39 using the following procedure: Cord blood samples were diluted 1:2 in PBS and layered on Ficoll® Paque Plus (Sigma-Aldrich by Merck, Darmstadt, Germany) to isolate mononuclear cells ...
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bioRxiv - Biochemistry 2023Quote: ... Fifty μl of a suspension in water of GA-fixed cells were mixed with 50 μl of 2% osmium tetroxide in water (Cod. 75632-5ML, Merck Life Science S.r.l., Milan, Italy). After 30 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... samples were acidified to pH 2 with formic acid and desalted with pipette tips packed with six layers of C18 Empore™ SPE Disks (Merck, cat. no.66883-U) and dried in a Speed Vac at 45 °C for 1 hour ...