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Citations for Merck :
1951 - 2000 of 2161 citations for 6 chloro 4 hydroxyquinoline 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The next day the cleaved protein was collected and concentrated by spinning the sample at 3500 RPM at 4°C using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... A 6:4 ratio of 0.1 % Oil-Red-O in isopropanol to 4 mL distilled water was prepared and passed through a 0.22 □m syringe filter (Merck: Z260479) before use ...
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bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Biochemistry 2024Quote: ... leaf punches were taken with a 4-mm biopsy punch and floated in 100 μL of H2O with 20 μM coelenterazine (Merck), using individual cells of a white 96-well white bottom plate (Greiner F-Boden ...
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bioRxiv - Molecular Biology 2023Quote: ... 10,000 cells were seeded in 12-well dishes and co-infected the next day with 4EBP1 and 4EBP2 shRNA viruses in medium supplemented with 4 µg/ml polybrene (Merck). Cells were grown until confluency and reseeded into 6-well dishes prior to addition of 1 µg/ml puromycin (InvivoGen ...
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bioRxiv - Neuroscience 2023Quote: ... heated to 37°C with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin79 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck), 100 µM jasplakinolide (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin- labeled probes and biotin-labeled probes were detected with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/1,000 diluted streptavidin conjugated with DyLight 549 (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... equipped with a LiChrospher® 100 RP-18 (125 mm x 4 mm, 5 µm) C18 reversed-phase column (Merck). The elution solvents consisted of ultrapure water with 0.1% formic acid (A ...
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bioRxiv - Genomics 2023Quote: ... 4 % DDT and 2 % clothianidin-impregnated papers prepared in acetone and Dow Corning 556 Silicone Fluid (BHD/Merck, Hesse, Germany). Flies overexpressing CYP6Z2 were also exposed to the primary product of hydrolysis of pyrethroids ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled fractions were diluted with 2 vols of KPEM buffer to achieve a final concentration of 125 mM KCl and then incubated for 1 hr with 4 mL anti-FLAG monoclonal antibody conjugated resin (M2 agarose, Merck) using rolling ...
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at low density following incubation with DNA or siRNA 24h prior to fixation with 4% paraformaldehyde (PFA, Merck) for 15min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
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bioRxiv - Developmental Biology 2023Quote: Upper and lower arms were harvested from 4 cm double reporter axolotls (Alx4:mCherry_Hand2:EGFP) and dissociated into single cell suspensions using Liberase TM enzyme (Merck 05401127001) as described in 49 ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Molecular Biology 2024Quote: Candidate genes were amplified from Drosophila melanogaster ovary cDNA library using KOD Hot Start DNA Polymerase (Merck Millipore, 71086-4) and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation (20.000xg, 15 min, 4°C) and protein concentration was determined by Direct Detect® spectrometer (Merck). The proteasome activity was measured using a 96-well plate ...
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bioRxiv - Genomics 2024Quote: ... About 2 mL of supernatants were then transferred to ultrafiltration tubes (Amicon® Ultra-4 Centrifugal Filter Unit 10K, Merck). 2 mL of TE buffer was added ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Biochemistry 2019Quote: ... The fractions corresponding to the single peak were pooled and concentrated using an Amicon Ultra −15 centrifugal filter (3 kDa, Merck Millipore Ltd. Ireland). Copper-(I ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to buffer B without imidazole using 3 kDa Amicon® centrifugal filters (0.5 ml size) (both from Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2020Quote: ... at different concentrations (20 mM, 200 mM and 500 mM) and concentrated using centrifugal ultrafiltration devices (3 kDa cut-off) (Merck India Pvt. Ltd.). This was followed by Gel filtration chromatography using Superdex 200 Increase 10/300 GL column (GE Lifesciences ...
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bioRxiv - Cancer Biology 2019Quote: ... the plasmid construct of β-galactosidase was co-expressed in equal amounts in wells and expression was measured in the presence of the ONPG substrate (Merck, 369-07-3). The reaction was stopped with 1M Tris-Cl (pH 11 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... The fixing solution was aspirated and wells washed 3 times in a washing buffer composed of DPBS containing 0.5% of BSA (Merck Life Science, Gillingham, UK). The washing buffer was left in the wells for 10 minutes following the last wash ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Cell Biology 2020Quote: ... Stag-Tulp3 was then pulled down from the TEV digestion eluates by overnight 4°C rotational incubation using S-protein agarose beads (Merck, #69704). Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... Mouse peritoneal macrophages (MPMs) were isolated from mice 4 d after the intraperitoneal injection of thioglycollate (Merck & Co.; Kenilworth, NJ, USA) as described previously(Zhang et al. ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... the following antibodies were treated to the organoids and the tissue slices at 4°C overnight: proSFTPC (1:200; Merck, AB3786), E-cadherin (1 ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C with a SW28 rotor in a Beckman XL-70 centrifuge or concentrated using an Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore). Pellets were resuspended in 500 µL PBS ...
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bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
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bioRxiv - Neuroscience 2020Quote: ... cryoprotected for 48 h in 20% sucrose at 4°C and then snap frozen in isopentane (2-methylbutane, Merck GmbH, Austria) for 3 min at −60°C ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing monomeric I-Ek/MCC(ANP)-biotin complexes were concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK), snap frozen in liquid nitrogen and stored in 1x PBS at −80 °C ...
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bioRxiv - Biophysics 2020Quote: ... dialyzed against Milli-Q water and concentrated to 4 mg ml-1 using Amicon Ultra centrifugal filter units with Ultracel-50K (Merck Millipore). UMODe filaments were obtained by limited digestion of UMODfl with elastase (Jovine et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...