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Citations for Merck :
151 - 200 of 1172 citations for Nipah Virus Glycoprotein F Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... nucleotides in a 0.5 μl reaction mixture were separated via thin layer chromatography using TLC PEI Cellulose F plates (Merck Millipore) and 1 M LiCl as solvent ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Microbiology 2020Quote: ... Purified Vag8 was concentrated and the buffer was exchanged to buffer F (50 mM Tris-HCl pH 8.0, 150 mM NaCl) by ultrafiltration (Amicon Ultra, Merck-Millipore).
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
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bioRxiv - Cell Biology 2024Quote: ... The explants were allowed to attach to the feeder layer before epithelial cell medium (ECM) containing Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham (DNEM-F12, #D8062, Merck) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2024Quote: ... The next day differentiation was induced in DMEM/F12 (Dulbecco’s Modified Eagle’s Medium/ Nutrient Mixture F-12 Ham; Merck Life Sciences), 1X Glutamax (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Immunology 2022Quote: ... Animals were randomly assigned to be treated with either 170,000 IU/kg human recombinant interferon alpha-2a (Merck), or BSA/saline (0.9% NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
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bioRxiv - Microbiology 2019Quote: ... faecal samples were added to 10 ml of Buffered Peptone Water (Fluka Analytical, 08105-500G-F) supplemented with 10 μg/ml Novobiocin (Merck, N1628), and incubated overnight at 37°C with shaking at 220 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... a micro-infusion pump (iPRECIO SMP-200, ALZET Osmotic Pumps) with 15 IU/kg human follicle-stimulating hormone (hFSH, Gonal-f; Merck Biopharma) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were washed 3x with PBS before incubation in a solution of anti-Rabbit-IgG-ATTO 594 (Merck, #77671-1ML-F) as secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... pyrene–labeled phalloidin–stabilized WT or mutant F–actin and the myosin solutions were treated with apyrase (Merck KGaA, Darmstadt, Germany) to remove residual nucleotide ...
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bioRxiv - Neuroscience 2019Quote: ... the rats were treated with either 170,000 IU/kg IntronA (human recombinant interferon-alpha 2b, Merck Sharp & Dohme Limited), or saline (0.9% NaCl ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Biophysics 2022Quote: ... was washed three times in Tris-buffer (pH 7.48) and resuspended in 1 %w/v Pluronic® F-127 (Merck Chemicals GmbH, Germany) Tris-Buffer.
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bioRxiv - Physiology 2020Quote: ... Compounds were identified based on their mass spectra in comparison to those of an alkane standard mixture (C10-C40, Merck 68281-2ML-F), by comparison of their retention times and fragmentation patterns to published Anopheles gambiae CHC mass spectra (Balabanidou et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Leuplin for Injection Kit), a micro-infusion pump (ALZET Osmotic Pumps, iPRECIO SMP-200) with 15 IU/kg human FSH (hFSH, Merck Biopharma, Gonal-f) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...