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Citations for Merck :
151 - 200 of 679 citations for B Raf Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Cell Biology 2021Quote: ... in 20 mL 1 N HCl) and Solution B (0.5 g Ammonium molybdate (277908, Merck) in 7 mL 4 N HCl ...
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bioRxiv - Immunology 2022Quote: ... and endpoint B) added with 500 µl of RPMI 1640 culture medium with cyanocobalamin (Merck) to a final concentration of 1 nM ...
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bioRxiv - Biochemistry 2024Quote: ... Calibration of the mass spectrometer was achieved using [Glu1]-Fibrinopeptide B (50 fmol/μL, Merck) and the instrument was operated at a source temperature of 80 °C with the capillary set to 3 kV.
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bioRxiv - Immunology 2023Quote: ... and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG, #A0545). Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cell Biology 2021Quote: ... 1000 units/ml of recombinant Leukemia Inhibitory Factor (LIF, Interchim, #8V6280) and 0.1% β-mercaptoethanol (Merck, #M6250) on gelatin-coated plates ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2022Quote: ... anti-Gsx2 (rabbit; Merck); anti-Histone H3 (phospho S10 ...
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bioRxiv - Plant Biology 2021Quote: ... or rabbit (Sigma Merck) anti-DIG antibodies (diluted 1:100 ...
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bioRxiv - Microbiology 2024Quote: ... rabbit anti-C4BPA (Merck), or rabbit anti-A2M (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... cells were dissociated by using an Enzyme-Free Cell Dissociation Solution (S-014-B, Merck Millipore) and washed twice in wash buffer (50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were re-plated in serum- free ESGRO Complete Clonal Grade medium (Merck, Cat# SF001- B). The list of 4DN sample IDs is provided in Supplemental Table 6.
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bioRxiv - Physiology 2023Quote: ... through which the retinal vasculature was injected with 1 mg/ml sulforhodamine B (479 Da; Merck) in Krebs solution ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Immunology 2022Quote: ... Animals were randomly assigned to be treated with either 170,000 IU/kg human recombinant interferon alpha-2a (Merck), or BSA/saline (0.9% NaCl ...
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bioRxiv - Pathology 2019Quote: ... the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA, Darmstadt, Germany 51) or polyclonal anti-CG antibody (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... the strain was grown on solid King’s medium B (Pseudomonas agar F, Merck KGaA, 64271 Darmstadt, Germany) supplemented with 50 μg mL−1 rifampicin at 25°C in the dark for 3 days ...
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bioRxiv - Synthetic Biology 2021Quote: ... with Hygromycin B resistance used as the selection marker as previously described.[49] Spermidine (Merck Life Sciences) was used to precipitate DNA onto gold particles of 0.3-3µm diameter (ChemPur) ...
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bioRxiv - Biophysics 2020Quote: ... Then His-tag containing OpuAC was introduced to the flow cell (in buffer B supplemented with 10 mM of (±)6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; Merck) as a photostabilizer[20] ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were grown for 48h in serum-free ESGRO Complete Clonal Grade Medium (Merck, Cat# SF001- B) supplemented with 1000 U/ml LIF ...
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bioRxiv - Cell Biology 2022Quote: ... at a final concentration of 5 μg/ml in M2 medium or Latrunculin B (428020-1MG, Merck) at a final concentration of 5 μM in M2 medium (to disrupt F-actin) ...
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bioRxiv - Immunology 2023Quote: ... and interacting infected cell-invaded B cell doublet images acquired with ImageStream X MKII (Amnis/Merck Millipore) were analyzed by IDEAS 6.3 (Amnis/Merck Millipore/Luminex).
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-CRTAC1 (Merck Millipore), goat anti-IgSF8 (R&D Systems) ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit anti-Olig2 (Merck, AB9610) and chicken anti-GFAP (AB4674 ...
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bioRxiv - Microbiology 2019Quote: ... Rabbit polyclonal JHDM1B/KDM2B (Merck Millipore ...
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bioRxiv - Zoology 2022Quote: ... or normal rabbit IgG (Merck) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and Rabbit IgG (Merck, #03198) per 1.5 million cells ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... rabbit anti-V5 antibody (Merck) or rabbit anti-HA antibody (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... rabbit-Sox9 (1:500; Merck), rabbit-Calbindin (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... and rabbit-HRP (GENA9640V, Merck) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-CDK5RAP2 (Merck Millipore), rabbit anti-CEP290 (Novus Biologicals) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Neuroscience 2019Quote: ... the rats were treated with either 170,000 IU/kg IntronA (human recombinant interferon-alpha 2b, Merck Sharp & Dohme Limited), or saline (0.9% NaCl ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were blocked with 5% milk in Tris buffered saline (TBS) and probed with antibodies to HCMV IE1 and IE2 (Merck Millipore; MAB-8131; 1/5000), pp52 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... washed with buffer B to stop the spheroplasting reaction and then washed into 10 % formamide (Merck Millipore S4117) in 2× SSC.
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bioRxiv - Physiology 2020Quote: ... all the birds were challenged with the Coccivac B-52 live vaccine (Merck Animal Health; 1.3× recommended dose). The vaccination was completed at d 5 to facilitate uniform intake of coccidian oocysts by the birds ...
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bioRxiv - Plant Biology 2019Quote: ... 0.8 % phytoagar (Duchefa) and 10 µg mL-1 Glufosinate-ammonium or 25 µg mL-1 hygromycin B (Merck) for herbicide selection ...
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bioRxiv - Genomics 2021Quote: ... with A pestle ~10 times following B pestle ~20 times in lysis buffer [5 mM CaCl2 (Merck, A546282), 3 mM Mg(Ac)2 (Roth ...
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bioRxiv - Immunology 2020Quote: ... Primed neutrophils were obtained by treating the cells for 25 mins with 10 μM cytochalasin B (Merck, #C6762) and 100 nM N-Formylmethionyl-leucyl-phenylalanine (fMLF ...
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bioRxiv - Cell Biology 2023Quote: ... pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...