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Citations for Merck :
1851 - 1900 of 2518 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The culture was grown for 2 hr at 37°C and stocks were made from the original plate by adding glycerol (Merck & Co., USA) to the final concentration of 15% and stored at -70°C ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Plant Biology 2023Quote: ... An aliquot of each sample was subjected to TLC using a silica gel 60 F254 aluminum TLC plate (Merck Millipore, Burlington, USA), using a running buffer containing ethyl acetate/acetic acid/methanol/formic acid/water at a ratio of 80:40:10:10:10 (v/v) ...
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bioRxiv - Synthetic Biology 2023Quote: ... All moisture-sensitive reactions were carried out in oven-dried glassware under N2 Thin-layer chromatograms (TLC) and flash chromatography separations were respectively performed on precoated silica gel 60 F254 plates (Merck, 0.25 mm) and on silica gel 60 (230-400 mesh) ...
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bioRxiv - Microbiology 2023Quote: Quantification of polar lipids content on HPTLC was carried out as follows: Lipid extracts and polar lipids standards at varying concentrations were applied onto silica gel 60 F254 HPTLC plates (Merck KGaA, Germany) using an ATS 5 automatic TLC sampler (Camag ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM DTT were heated in a LightCycler® 480 Multiwell white 384-well plate in a Roche LightCycler® 480 II from 20 to 80°C in the presence of SYPRO™ Orange (Merck) diluted 1:160.
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
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bioRxiv - Biochemistry 2019Quote: ... The fractions corresponding to the single peak were pooled and concentrated using an Amicon Ultra −15 centrifugal filter (3 kDa, Merck Millipore Ltd. Ireland). Copper-(I ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to buffer B without imidazole using 3 kDa Amicon® centrifugal filters (0.5 ml size) (both from Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
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bioRxiv - Biophysics 2020Quote: ... at different concentrations (20 mM, 200 mM and 500 mM) and concentrated using centrifugal ultrafiltration devices (3 kDa cut-off) (Merck India Pvt. Ltd.). This was followed by Gel filtration chromatography using Superdex 200 Increase 10/300 GL column (GE Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck, Buchs Switzerland). Memofl/fl littermates without Cre but treated with tamoxifen served as controls ...
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bioRxiv - Cancer Biology 2019Quote: ... the plasmid construct of β-galactosidase was co-expressed in equal amounts in wells and expression was measured in the presence of the ONPG substrate (Merck, 369-07-3). The reaction was stopped with 1M Tris-Cl (pH 11 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fixing solution was aspirated and wells washed 3 times in a washing buffer composed of DPBS containing 0.5% of BSA (Merck Life Science, Gillingham, UK). The washing buffer was left in the wells for 10 minutes following the last wash ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: Multiplex analysis based on the xMAP Luminex technology was performed with the use of a kit for MILLIPLEX MAP Porcine Cytokine/Chemokine (magnetic) kit # PCYTMG-23K-13PX (Merck), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 20 million cells using the Magna ChIP A/G Kit (One-day chromatin Immunoprecipitation Kits, Merck) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... These samples were stored in the dark and analyzed colorimetrically using a commercial kit (Spectroquant Sulfide kit, Merck, Schaffhausen, Switzerland). Total particulate nitrogen and carbon were determined by filtration of 100 to 220 mL lake water on pre-combusted (400°C ...
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bioRxiv - Molecular Biology 2020Quote: ... An enhanced chemiluminescence (ECL) detection kit (Merck Millipore) was used to develop the membranes ...
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bioRxiv - Cell Biology 2022Quote: ... the Senescence Cells Histochemical Staining Kit (Merck, UK) was used according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: The Muse Ki67 Proliferation kit (Merck, Princeton, USA) was used to detect proliferating and non-proliferating cells based on Ki67 expression ...
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bioRxiv - Microbiology 2022Quote: ... Live/dead Double Staining Kit (Merck Life Sciences) was used to stain cells followed by microscopy ...
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bioRxiv - Microbiology 2024Quote: ... ECL kits and absolute ethanol were from Merck-Millipore (Germany) ...
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bioRxiv - Cancer Biology 2024Quote: ... as per the manufacturer’s protocol (Merck EZChIP kit). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: A multiplex assay kit (HNABTMAG-68K, Merck Millipore) was used to quantify Aβ40 ...
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bioRxiv - Molecular Biology 2024Quote: ... LCAT activity assay kit (Sigma-Aldrich MAK107, Merck) was used according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... contained in the Kap2G Robust PCR kit (MERCK) per sample and 9 μl added to each well need to be used in a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... difficile using the succinate colorimetric assay kit (Merck) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2019Quote: ... 3x 5 min in H2O and further stained with 0.4% uranyl acetate (Serva) and contrasted with lead citrate (Merck). Grids were imaged with a transmission electron microscope LEO (Zeiss ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... also in with PBS containing 2.5%, bovine serum albumin (BSA; A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany). For visualizing neuronal membranes ...
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bioRxiv - Neuroscience 2020Quote: ... and were then blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were permeabilized and blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumors and lungs were paraffin-embedded and consecutive 5-μm sections were stained with hematoxylin-eosin purchased from Merck.
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich; Merck KGaA) for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was clarified by centrifugation (500g, 5 min) and filtration (0.45 μm, Millex-HV, Merck Millipore (Burlington, MA)) ...
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bioRxiv - Biochemistry 2022Quote: ... diluted 1:1 with dilution buffer (50 mM Hepes pH7.5, 150 mM NaCl, 5 mM MgCl2, 0.625 U/ul Benzonase (Merck, 70746)) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES pH 7.5, 400 mM NaCl, 20 mM imidazole, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...