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Citations for Merck :
1851 - 1900 of 1975 citations for Goat Anti Human IgM HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... and SDS-PAGE and immunoblots were performed by standard methods using a mouse monoclonal anti-MYC antibody (clone 4A6, 05-724, Merck).
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... and the remaining supernatant was used for chromatin immunoprecipitation and mixed with magnetic beads (60 μl) coated with anti-FLAG M2 antibody (Merck). Immunoprecipitation was performed overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Double staining with Fluro Jade C and anti-SARS-COV2 Nucleocapsid antibody were performed by following the protocol from the Fluro Jade C supplier (AG325, Merck) with some minor modifications ...
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bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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bioRxiv - Immunology 2021Quote: ... plates were fixed with 1:1 acetone and methanol Anti-S1 rabbit polyclonal antibody (Novus) was added to each well and incubated for 1Hr followed by incubation with FITC labelled anti-rabbit antibody (Merck). Fluorescence images were captured using an inverted microscope (ZeissAX10).
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bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were incubated with anti-HA agarose beads (20 μl of beads per 1 mg of proteins) (Merck, Darmstadt, Germany) for 2 h ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse Cy3 conjugated secondary antibody (cat no. AP124C) and anti-rabbit Cy3 conjugated secondary antibody (cat no. AP132C) were procured from Merck. Anti-rabbit Alexa Fluor 488-conjugated secondary antibody (cat ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... Fragmented RNA was subjected to two rounds of m6A immunoprecipitation for 2 hours each using an anti-m6A antibody (ABE572, Merck) previously conjugated to protein-A magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... was assessed before and after induction with Doxycycline (1 μg/mL) by Western blot analysis using an anti-FANCJ (cat. B1310, Merck) and/or an anti-Flag antibody.
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for ChIP analysis: Mouse anti-RNA polymerase II antibody clone CTD4H8 (Merck Millipore, 05-623), Rabbit anti-NF-kB p65 antibody clone D14E12 (Cell Signalling ...
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bioRxiv - Neuroscience 2022Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were run on SDS-PAGE and the expression of IDO1 was analyzed with a mouse anti-IDO1 antibody (clone 8G-11, Merck). Mouse monoclonal Ab against β-tubulin (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated with an anti-sera peptide based polyclonal rabbit GLK2-antibody (1:3,000) and a peroxidase-conjugated anti-rabbit antibody (1:10,000) (Merck, Darmstadt, Germany) for 1 h at room temperature in TBST [Tris-HCl 20 mM (pH 7.6) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) in 1× PBS for 20 min at room temperature and then incubated with the anti-ADAR antibody HPA051519 (Merck) diluted 1:200 with PBS for 12 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin- labeled probes and biotin-labeled probes were detected with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/1,000 diluted streptavidin conjugated with DyLight 549 (Vector Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were incubated for 1 h at RT with a mouse anti-2A primary antibody (cat. no. MABS2005, Merck) diluted 1:2000 in 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... the fixation procedure above with and without subsequent membrane permeabilization was used in infected/uninfected organoids that were then stained with a mouse monoclonal anti-mitochondria antibody Cy3 conjugate (Merck) incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Proximity ligation assays with Aurora A kinase and MP-GAP were performed in HeLa cells using mouse anti-Aurora A Kinase antibody (1:500, A1231 Merck), rabbit anti-MP-GAP antibody (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... the buffer was removed and 50 μl per well of monoclonal 3F10 anti-HA-Peroxidase (Merck Life Science, Gillingham, UK) 12.5 ng per ml in washing buffer was added and the plate incubated 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled fractions were diluted with 2 vols of KPEM buffer to achieve a final concentration of 125 mM KCl and then incubated for 1 hr with 4 mL anti-FLAG monoclonal antibody conjugated resin (M2 agarose, Merck) using rolling ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight with an anti-GFP rabbit antibody (0.5 μg/ml) (Tamamaki et al., 2000) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000; MAB3120, Merck Millipore). After a rinse ...
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bioRxiv - Evolutionary Biology 2023Quote: ... CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7, sc-13130, lot no # B1422) or control mouse IgG (12-371, Merck) in IP Buffer with 2X SDS buffer (100mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 µl of undiluted chromatin was incubated overnight at 4°C with Dynabeads M-270 Epoxy preincubated with anti-Tyr1P (MABE350, Merck), anti-Ser2P (C15200005-50 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were mounted using a mowiol-based mounting medium with DABCO (1,4-Diazabicyclo[2.2.2]octane) anti-fading agent (Merck Life Science SLU). Images were acquired using a Carl Zeiss-Axio Imager.M2 microscope and processed with ZEN 3.9 software (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... the localization was made visible by immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... A sample of the supernatant was collected as “input” and the remaining solutions were either mixed or separately incubated with previously washed Anti-Flag M2 beads (Merck) by tumbling for 150 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... chromatin samples cross-linked by formalin were enzymatically shared and incubated with rabbit anti-AcH4 antibody or isotype control antibody (Merck) at 4 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer of the anti-PTP1B antibody was exchanged to PBS-T using a Microcon-30kDa centrifugal filter unit (MRCF0R030, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were clarified by centrifugation and a minimum of 200μg of lysate was incubated with pre-washed EZview™ Red ANTI-FLAG® M2 Affinity Gel (F2426, Merck) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...