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Citations for Merck :
1801 - 1850 of 1920 citations for Anti ALKBH5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Developmental Biology 2021Quote: ... for 30min at RT and then incubated with either anti-pHH3 (Merck, #06-570, 1:500) or anti-Ascl1 (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg 6xHis-tag purified Mif2-6xHis-6xFlag was immobilized on anti-FlagM2 agarose beads (Merck) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in polyclonal rabbit anti-phospho-CREB (1:2000; cat. 06-519, Merck Millipore) in 0.3% Triton-X-100 in BSA/PBS for 48 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Flag pull down was performed incubating cleared cell lysates with Anti-flag M2 affinity gel (Merck) during 2h ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with 1:1,000 mouse anti-Rac1 (clone 23A8 05-389; Merck Millipore, USA) overnight at 4 °C and 3 h at RT and then washed 3 times with TTBS ...
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bioRxiv - Genomics 2022Quote: ... the membranes were probed (overnight at 4°C) with rabbit polyclonal anti-tyrosine hydroxylase (Merck, AB152) diluted in TBS/0.2% Tween/2% milk ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant filtered with 0.22 μM filter and mixed with anti-Flag M2 Agarose beads (Merck) for 2 h in a rotating wheel at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Biochemistry 2023Quote: ... ADP-ribose binding reagent (Anti-pan-ADP-ribose binding reagent, dilution 1 to 3000, MABE1016, Merck) or anti-RPS9 serum (dilution 1 to 10000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated 48 – 72 h with sheep anti TPH (1:1000, ab1541, Merck Millipore) primary antibody in carrier solution containing 1% NGS and 1% BSA in PBST at +4°C ...
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bioRxiv - Immunology 2019Quote: ... sections from the initial portions of sockets were incubated with primary antibody for Proliferating Cell Nuclear Antigen (PCNA) (#MAB424, Merck Millipore, Darmstadt, Germany) diluted to 1:150 in blocking solution and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Genetics 2022Quote: ... Incubation with appropriate secondary HRP-labelled antibody (less than 1h) was followed by detection with Immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were prepared and subjected to western analysis as described previously (49) using antibodies to the following: α-tubulin (T9026, Sigma-Aldrich, Merck, Inc.), ATF4 (11815 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were first incubated with anti-T7-tag conjugated with alkaline phosphatase (1:10000, Merck, #6999) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4°C with anti-KCC2 (1:200; Merck Life Sciences, Italy, #07-432) or anti-IGF-1R β (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of lysate was incubated overnight with 20µl anti-Flag M2 magnetic Dynabeads (Sigma-Aldrich/Merck) under shaking ...
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bioRxiv - Cell Biology 2021Quote: ... Respiratory syncytial virus RSV-G was immunoblotted with commercially available goat polyclonal anti-RSV serum (#AB1128, Merck). Coxsackievirus VP1 was immunoblotted with commercially available mouse monoclonal anti-VP1 (3A8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Phophorylated MEK was detected using anti-phospho MEK 1/2 (residues 218/222 and 222/226, MERCK, catalogue No ...
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bioRxiv - Physiology 2022Quote: ... was used to identify the vasa recta capillaries and anti-neural-glial 2 (NG2; AB5320, Merck-Millipore) was used as a marker of pericytes ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 5 % sheep serum in MAB and incubated with anti-DIG conjugated to Peroxidase (1/50; Merck) in 1 % blocking reagent diluted in MAB for 3 days at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... NBP1-92693AF647) and the oligodendrocyte transcription factor OLIG2 (Anti-OLIG2 clone 211F1.1 mouse mAb, Merck Millipore, MABN50A4). Samples were kept on ice throughout the isolation and staining procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were incubated with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/2,000-diluted streptavidin conjugated with alexa405 (Vector Laboratories ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Microbiology 2023Quote: ... Goat Anti-Rabbit IgG HRP conjugate (632131/Merck/Massachusetts, USA, 1:10,000 were procured from Merck (USA). Mouse ß-Actin (3700S/ 1:1000/Cell Signalling technology/Massachusetts ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemistry was performed on colon sections as described earlier on brain sections58 using additional antibodies (α-Syn: ab27766, Abcam or L001, Enzo; NeuN: MAB377, Merck; MAP2: ab70218 Abeam; TH: 657012, Merck; GFAP: Z0334, Dako).
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bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... the permeabilized cells were incubated for 1 hour with anti-phospho-S10 Histone H3 (06-570, Merck Millipore) in PBS containing 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Biochemistry 2020Quote: ... anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) clone 3E10 (Merck, 04-1571-I, 1:5,000), and anti-RNA polymerase II CTD repeat YSPTSPS antibody clone 8WG16 (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... and mouse monoclonal anti-APP (1:500, 22C11; Cat#Mab348, RRID: AB_94882, Merck Millipore/Sigma, Burlington, MA, USA) antibodies were purchased from the indicated suppliers ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were incubated overnight under rotation at 4°C with ANTI-FLAG® M2 Affinity Gel beads (Merck) to pull down FLAG-containing SNAP-GLP-1R ...
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bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal somata were visualized by NeuN immunolabeling (1:1000, monoclonal mouse anti-NeuN IgG, #MAB377, /Merck, Darmstadt, Germany) in CRH-ires-cre ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP (AP182P, Merck Millipore) or donkey anti-mouse IgG HRP (AP192P ...
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bioRxiv - Pathology 2022Quote: ... respectively an anti-rat IgG conjugated to alkaline phosphatase (1:500) (Sigma-Aldrich®, Merck KGaA, Darmstadt, Germany), for 60 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were normalised across samples and were added to 100 μl anti-FLAG M2 affinity gel (Merck) Micro Bio-Spin columns (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μl of IP buffer and 10 μl of anti-FLAG M2 Affinity Agarose Gel (A2220, Merck Millipore) were added to the 200 μl of lysate ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined and both fractions were incubated with Anti-FLAG® M2 Magnetic Beads (Merck, M8823). Beads were washed once then each sample was split for MS ...