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Citations for Merck :
1751 - 1800 of 2125 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: chicken anti-GFAP 1:500 (ab5541, Merck Millipore, Darmstadt, Germany), mouse anti-AT8 1:1000 (ab5541 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Primary antibodies used were anti-tyrosine hydroxylase (anti-TH; cat.#AB152, Merck Millipore, Burlington, MA, USA), anti-total-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (anti-tAMPAR ...
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bioRxiv - Genetics 2024Quote: ... and finally incubated with secondary goat anti-mouse 1:5000 HRP antibody (Merck-Millipore #12-349). Images were acquired on the Alliance Uvitec (Uvitec Ltd ...
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bioRxiv - Neuroscience 2024Quote: Rodent as well as human brain slices were stained using the antibodies NeuN (MAB377, MilliporeSigma (Merck), 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated for 1 h with anti-c-myc tag antibody (Merck #05-724) diluted [1:2500] in a blocking buffer and washed 3 times with TBSt ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was then incubated with mouse anti-mouse (HRP) antibodies (diluted at 1:10,000, Merck) for an hour ...
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bioRxiv - Cell Biology 2024Quote: ... prior to overnight incubation with the following primary antibodies: anti-βIII-tubulin (1:100, Merck KGaA), anti-SOX17 (1:100 ...
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bioRxiv - Genomics 2024Quote: ... Immuno-detection was done with anti-ssDNA antibody (1/100 dilution, clone 16-19, Merck Milipore), the mouse anti-BrdU antibody (1/20 dilution ...
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS- PAGE and those containing pure tag free CBX8 protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer consisting of 20mM MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated to the desired concentration using centrifugal filters (#UFC800308; Amicon® Ultra centrifugal filters, Ultracel® −3K or larger; Merck Millipore Ltd.)
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bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
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bioRxiv - Biochemistry 2024Quote: ... The fractions containing the monodisperse protein were pooled and further concentrated by ultrafiltration using 10000 Da MWCO Amicon ultrafiltration devices (Merck Life Science, Darmstadt, Germany) at 4000 g and 4 ◦C on a Heraeus Multifuge X1R centrifuge (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and a horseradish peroxidase (HRP)-conjugated donkey anti-goat IgG as secondary antibody (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were probed with a secondary antibody: either anti-rabbit-HRP (1:5,000; Merck Millipore AP132P), anti-mouse-HRP (1:5,000 ...
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bioRxiv - Genomics 2020Quote: ... incubated with primary antibodies (anti-AP-1 Thermo Scientific™ #MA5-15172, anti-Sp1 ChIPAb+™ Merck #17-601 ...
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bioRxiv - Cell Biology 2020Quote: ... or HRP-conjugated monoclonal anti β-actin antibody (1:20,000, Sigma-Aldrich, Merck, St. Louis, Missouri, USA) for a loading control ...
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bioRxiv - Molecular Biology 2022Quote: ... Acetylation was evaluated with Western blot probed with anti-acetyl lysine antibodies (1:800 dilution) (Sigma/Merck). Mass spectrometry outsourced to Mass Spectrometry Laboratory IBB PAN ...
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bioRxiv - Neuroscience 2022Quote: ... and one of the following primary antibodies: mouse anti-GAD67 for staining GABAergic cells (Merck & Co., MAB5406), goat anti-Substance P for staining D1-containing cells (Santa Cruz ...
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bioRxiv - Immunology 2022Quote: ... IL-6 and IL-1RA was performed using the Luminex color-coded antibody-immobilized beads from Merck Millipore following the manufacture’s indications ...
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bioRxiv - Neuroscience 2020Quote: ... glass slides were incubated with an anti-DIG-AP antibody (Anti-Digoxigenin-AP, Fab fragments; Merck, Germany) overnight in a humidified chamber ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were captured on the same chip by a previously immobilized anti-Fc antibody (Merck Life Science S.r.l). Anti-FLAG or anti-Fc antibodies were immobilized by classical amine coupling chemistry (29 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: monoclonal mouse anti-puromycin (clone 12D10, MABE343, Merck Millipore; 1:1000), polyclonal rabbit anti-Tau (T6402 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at 4°C with primary antibodies against puromycin (1:500, MABE343, Merck Millipore), βIII tubulin (1:500 ...
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bioRxiv - Immunology 2020Quote: ... and the supernatant was taken for immunoprecipitation with 2 μg of anti-ubiquitin antibody (FK2, Merck-Millipore) for 12 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1p was detected using a rabbit anti-phospho H4/H2A S1p antibody (07-179; Upstate/Merck-Millipore) at a dilution of 1:4000 ...
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bioRxiv - Genetics 2020Quote: ... membrane was divided into two halves and probed with either monoclonal anti-(c-Myc) antibody (Merck Millipore) or anti-α-tubulin antibody (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... Immune complexes were detected using HRP-conjugated secondary antibodies and the Immobilon Western Chemiluminescent HRP Substrate (Merck).
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bioRxiv - Neuroscience 2020Quote: The following primary antibodies were used: rabbit polyclonal anti-MuSK (1:1,000; Merck Millipore, catalog no. ABS549), anti-HA.11 (1:100 for immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... c-Src was detected using anti-Src (GD11 clone) antibody (1:200, Merck Millipore, Mouse, 05-184). Phosphorylated c-Src was assessed using anti-phospho-Src Tyr 418 antibody (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary antibodies and working dilutions used in this study were: SOX9 (Merck millipore, AB5535, 1:600), FOXL2 (abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were immunolabelled with anti-phospho-Histone H3 (Ser10) primary antibody (1:500, 06-570 Merck millipore), washed for 0.5 day in PBS/0.1% Tween-20/10μg/ml heparin (PBSTwH ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... cells were incubated with an IF-optimized EMRE antibody (anti-SMDT1/C22orf32/EMRE; #HPA060340, 1:250; Merck), washed 3 times in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used for Western blotting were Goat-Anti-mouse IgG HRP conjugated (catalog #12-349; Merck) and Goat-Anti-rabbit IgG HRP conjugated (catalog #12-348 ...
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bioRxiv - Plant Biology 2024Quote: ... the blots were incubated with primary antibodies α-FLAG (Merck, F1804, 1:1000], α-TPLATE34(1:1000), α-AtEH1/Pan135(1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with the secondary antibodies (Alexa 647-conjugated donkey anti-guinea pig, 1:200, AP193SA6, Merck Chemicals ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with the secondary antibodies (Alexa 647-conjugated donkey anti-guinea pig, 1:200, AP193SA6, Merck Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for immunofluorescence in Drosophila: mouse anti-puromycin (clone 12D10, Merck Millipore) at 1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... gastruloids were incubated with secondary antibodies and 4’,6-diamidino-2-phenylindole (DAPI, 1 µg/mL, Merck) in OWB-SDS at 4°C overnight ...