Labshake search
Citations for Merck :
1751 - 1800 of 4681 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-β-tubulin (1:1000; clone KMX-1; Merck Millipore, Massachusetts, USA), mouse anti-acetylated α Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour with mEM48 (1:500, Merck Millipore, MAB5374) antibody at room temperature in TBS containing 0.1% TritonX-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-acetylated α-tubulin (clone 6-11B-1, Merck; ExM, 1:250), mouse anti-acetylated α-tubulin (T7451 ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse monoclonal anti-NUCB2/nesfatin-1 antibody (1:50; Merck Millipore, MABS1164), and b ...
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bioRxiv - Bioengineering 2024Quote: ... Part 1 of the precursor solution contained 20 mg mL-1 fibrinogen (Merck) diluted in DMEM/F12 with HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-γ-H2AX (Merck, 05-636, 1 mg/ml, dilution 1:1000). For the secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... were either immersed in a 1:1 mixture of 30% hydrogen peroxide (Merck) and concentrated sulfuric acid (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-DNA Ligase 1 (Sigma Aldrich, Merck, clone 5H5, MABE1905; 1:500); mouse anti-PARP1 (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: Two lipids (18:1-18:0 PC, 18:0-18:1 PC) (Merck) and stored at -20°C until sample preparation ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 dilution and nuclear stain DAPI (1 μg/ml, Merck, D9542) at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:2000 and/or DAPI (at 1:1000, ready-made solution, Merck).
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bioRxiv - Developmental Biology 2023Quote: ... they were incubated in 100 μg/mL DNAse 1 (1 mg/mL, Merck) in a concentration of 1000 cells/μL for 15 mins at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μl polybrene (Merck, TR-1003-G, stock solution at 1 mg/ml) and 1 μl Y-27362 (Calbiochem ...
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bioRxiv - Microbiology 2020Quote: ... Samples were pre-treated with (1) 36 µl of Lysozyme [1% w/v in 1% PBS – 37°C/30min with intermittent shaking] (Merck KGaA, Germany) and then with (2 ...
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bioRxiv - Neuroscience 2022Quote: Small and large intestines were dissected from E14 embryos and digested with 1 mg/ml DNAse 1 (AppliChem A3778) and 1 mg/ml collagenase A (Merck Millipore 10103586001) in DMEM-F12 at 37 °C while shaking ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 1 hour with 1% BSA blocking solution containing DAPI at 1:4,500 (Merck Life Sciences, Cat #: D9542), phalloidin at 1:350 (Merck Life Sciences ...
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bioRxiv - Physiology 2024Quote: ... Rabbit α-mouse Calmodulin (5μg mL-1, CellSignaling), rabbit α-mouse β-actin (1μg mL-1, CellSignaling) and mouse α-mouse GAPDH (1μg mL-1, Merck/Millipore), goat-α-mouse IRDye 680RD ...
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bioRxiv - Synthetic Biology 2021Quote: ... Seed cultures from glycerol stocks were inoculated 20 hours overnight in 5 mL Invitrogen Luria Broth Base LB medium (ThermoFischer, USA) and supplemented with Kanamycin sulfate (Merck, Germany) (50 μg/mL) ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2021Quote: ... Color was developed in the dark for 5-10 min before adding 50 µL of 2 M H2SO4 stop solution (Merck, Germany). Optical density was read at wavelength 450 nm using the microplate reader (SpectraMax ID3 ...
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bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Bioengineering 2021Quote: ... INS-1 cells in bioprinted grid scaffolds were stained with thiazolyl blue tetrazolium bromide (MTT) after 5 days in culture according to the manufacturer’s protocol (Merck, Darmstadt, Germany). Proliferation was determined using an ATP-based assay with luminescent readout (CellTiter-Glo® 3D Cell Viability Assay ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 15 minutes in 95% and 100% ethanol and 5 minutes in hexamethyldisilazane (HMDS, Sigma-Aldrich, Merck, St Louis, USA). Samples were left to air-dry overnight in a biosafety cabinet prior to mounting on electron microscope stubs covered with carbon tape ...
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bioRxiv - Microbiology 2021Quote: ... The diluted solution was concentrated to approximately 5 mL using 30 kDa molecular weight cutoff Amicon Ultra centrifugal filters (Merck KGaA), passed through a Millex-GP syringe filter with 0.22 μm pore size (Merck KGaA) ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Microbiology 2020Quote: ... column using a nanoUPLC (nanoLC Ultra 1D plus, Eksigent, USA) and eluted (400 nL/min) with acetonitrile gradient (5%-80%) (LiChrosolv®, Merck) with 0.1% formic acid ...
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bioRxiv - Immunology 2020Quote: ... The pseudo-particle-containing supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... the HA-GlmS line was transfected with the pHGBrHrBl-1/2 construct in order to episomally express cytosolic GFP then selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). To generate Pfcerli2SLI-TGD ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and SsuT3-oE-R (5′-AGTCAG GGATCC CTA CAC CAC CTT CAC TTT GGT ACC) with KOD Hot Start DNA polymerase (Merck Millipore) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore). HEK-293 cells stably expressing hACE2 were seeded into 0.1% gelatin-coated 96-well plates (Greiner ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation for 5 minutes at 18 000 g the DNA pellet was washed twice with one mL 70% Ethanol (Merck Millipore) followed by 5 minutes centrifugation at 18 000g ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Systems Biology 2020Quote: TGF-β/Activin/Nodal signal inhibitor (SB) stock (4–5 mM): SB 431542 hydrate (#S4317-5MG, Merck & Co., Inc., NJ, USA) diluted in DMSO (#D2650-5×5ML ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated and diafiltrated against 20 mM phosphate buffer containing 500 mM NaCl and 20 mM imidazole (pH 7.4) using a Labscale TFF system equipped with a 5 kDa cut-off PelliconTM XL device (Merck Millipore, PXC005C50). His-tagged trimer Spike and monomeric RBD were captured using a 5 mL HisTrap FF crude column (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... for 30 min at 37 °C and washed with 5% FBS (EmbryoMax® ES Cell Qualified FBS, ES-009-B, Merck) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... to remove larger organisms and was filtered on board first through 5 μm and then through 0.2 μm polycarbonate filters (47 mm, Merck, Darmstadt, Germany) using pressurized air ...
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bioRxiv - Microbiology 2021Quote: ... and the remaining sample was centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2021Quote: ... they were centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Immunology 2022Quote: ... The remaining volume was spun down at 500x g for 5 minutes and resuspended in 12 ml FACS buffer (PBS [Gibco]+ 0.5% BSA [Merck] + 2mM EDTA). The suspension was transferred over a 70 μm SmartStrainer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation 100 μL of 2X STOP buffer (NaCl 340 mM, EDTA 20 mM, EGTA 4 mM, 5% Digitonin, 100 μg/mL RNase A (Merck, R6513), 50 μg/mL Glycogen (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml min−1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was washed once in 5 mL of Honda buffer then purified on a Percoll density gradient as follows: 2 mL of 75% Percoll (Merck, P7828) in Honda buffer topped with 2 mL of 40% Percoll in Honda buffer topped with the nuclei pellet resuspended in Honda buffer in a 15 mL tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC system Shimadzu Prominence (Shimadzu, Kyoto, Japan) was used with Chromolith 100 (5 μm) RP-18 endcapped column (Merck, Darmstadt, Germany), mobile phase was 60% methanol ...