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Citations for Merck :
1701 - 1750 of 2128 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Equal amounts of total protein (50 μg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of total protein (50 µg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Immunology 2023Quote: ... The eluted proteins were buffer exchanged into PBS and then concentrated using Amicon® 30 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9030).
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bioRxiv - Biophysics 2022Quote: ... equal amounts of GFP and SNAP-tagged recombinant WT-FUS protein (yielding a total protein concentration of 6 μM) were gently mixed into an aqueous solution of 20 % PEG-35 (Merck KGaA, Darmstadt, Germany) and 500 mM KCl (Merck KGaA ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were concentrated using an AmiconTm Ultra (molecular weight cut-off 30 kDa and 50 kDa) centrifugal filter device (Merck Millipore, Darmstadt, Germany). Fractions from all purification steps were collected and analyzed by SDS- PAGE.
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... to calculate the volume equivalent of 100 µg of protein before loading onto a 3 kDa molecular weight cutoff (MWCO) filter (Merck Millipore, Bayswater, Australia). Tryptic peptides were prepared using the FASP protocol as previously described (75) ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of proteins per sample were subjected to SDS-PAGE and transferred to Immobilon-PSQ PVDF transfer membrane (Merck Millipore, Darmstadt, Germany) with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... were supplemented with or without S100A8 or S100A8/9 recombinant protein and plated to 24 corning corstar ultra-low attachment multiple well plates (Merck, Cat. #CLS3473, Germany). Tumorsphere were imaged using IncuCyte S3 Live-Cell Imaging Analysis System (Ann Arbor ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Microbiology 2021Quote: ... with prior 1h incubation of the biotinylated oligonucleotide with the anti-G4 antibody BG4 (Merck Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... RNA:DNA hybrids were immunoprecipitated from total nucleic acids using 5µg of S9.6 antibody (MABE1095, Merck Millipore) in binding buffer (10mM Na2HPO4 pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution together with DAPI (0.01 mg/mL) (Merck Life Science) and incubated for 20 minutes at room temperature in a dark ...
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bioRxiv - Cell Biology 2021Quote: ... hybridized riboprobes were detected with alkaline phosphatase coupled rabbit anti-digoxigenin antibody (1:500; Merck 11093274910) for two hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and the appropriate primary antibodies from: goat anti-Choline Acetyltransferase (ChAT) (1:100) (Merck UK, ab144), chicken anti-GFAP (glial fibrillary acidic protein ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was captured on the chip by a previously immobilized anti-FLAG antibody (Merck Life Science S.r.l). S protein (Euprotein) ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody incubation was followed by 0.1% TBST wash and developed using an ECL kit (Merck). Densitometry analysis was used for quantitation of protein expression levels using Image J ...
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bioRxiv - Molecular Biology 2021Quote: The following antibodies and dilutions were used: CD9 (1:2000 dilution, clone #MM2/57, Merck Millipore), CD63 (1:500 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... the primary antibody (2.0µg/mL; anti-DNA:RNA hybrid, clone S9.6; monoclonal, mouse, IgG2ak, Merck Millipore, Germany) and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL ...
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bioRxiv - Genetics 2020Quote: ... and incubated with 1:3500 anti-FLAG antibody (monoclonal anti-FLAG M2; Merck KGaA, Darmstadt, Germany).
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with directly conjugated fluorescent antibodies in PBS/1% (w/v) BSA (Merck)/0.05% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-integrin β1 antibody (clone HUTS-4) was obtained from Chemicon (Merck Millipore, Billerica, MA). Rabbit polyclonal antibodies to fibronectin and fibrinogen were from Abcam (Cambridge ...
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bioRxiv - Neuroscience 2021Quote: ... 400 μl cell lysate were incubated with 4 μl HTT MAB2166 rabbit primary antibody (Merck, #MAB2166) for 2 hours at 4° C on a rotating device ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA; 17 hrs at 4°C). Similar immunolabeling steps were followed for the subsequent sequential staining ...
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bioRxiv - Bioengineering 2021Quote: ... Intracellular staining then performed by indirect immunofluorescence method with an adapted dilution of Nestin antibody (Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Modified and non-modified PCNA was detected using ANTI-FLAG® M2-Peroxidase (HRP) antibody (Merck).
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bioRxiv - Cell Biology 2021Quote: ... Acetylated histone 3 was detected using a polyclonal rabbit antibody (Merck, 06-599, 3260200, 1:500) and visualized using a donkey anti-rabbit Alexa Fluor 488 secondary antibody (Molecular Probes ...
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bioRxiv - Cell Biology 2023Quote: DNA-RNA Immunoprecipitation was performed using Anti-DNA-RNA Hybrid Antibody S9.6 (Merck Cat No MABE1095) as per Sanz and Chédin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Primary antibodies were diluted in the corresponding blocking solution anti-HA for ZnT9 (H3663, Sigma-Merck), and anti-GAPDH (ab8245 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The sections were incubated overnight by polyclonal anti-ChAT goat antibody (AB144P, Merck millipore, MA, USA), followed by a 3-h incubation with polyclonal Donkey anti-Goat Alexa flour 488 antibody (AB 2340428 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 μL magnetic beads were incubated with 5 μg of Ago-1 antibody (Merck, Millipore, Germany) or IgG antibody (Merck ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used: CD9 (1:2000 dilution, clone #MM2/57, Merck Millipore, Darmstadt, Germany), CD63 (1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies and dilutions were used: anti-GLUT1 (1:1000, Merck Millipore, 07-1401), anti-UCP1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then first incubated with mouse anti-PSD95 antibodies (1:100; Merck-Millipore, Cat. #MAB1596) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Bound antibodies were detected with the horseradish peroxidase (HRP) substrate o-phenylenediamine (Merck KGaA, Darmstadt, Germany). The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit polyclonal antibodies against ARHGEF2 phosphorylated at Thr-103 was produced against phosphopeptide C+LLRNNpTALQSVS (Merck) as previously described57 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Alexa Fluor 488-conjugated goat anti-mouse antibody (diluted at 1:200, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Genomics 2023Quote: ... incubated in the secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Merck 12-348) (1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used in immunofluorescence were anti-rabbit IgG–Rhodamine goat polyclonal (Merck, #SAB3700846, 1:500), anti-mouse IgG– Rhodamine rabbit polyclonal (Merck ...
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bioRxiv - Cell Biology 2022Quote: For western blot and immunofluorescence analysis following primary antibodies were used: Anti-Actin (mouse, Merck Millipore); Anti-ARP3 (mouse ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), Collagen I (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: anti-Ki-67 (rabbit, 1:300, Merck, Cat. no. AB9260), anti-SOX2 (goat ...
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bioRxiv - Microbiology 2022Quote: ... The anti-Coxsackievirus B3 monoclonal antibody was obtained from Merck (clone 280-5F-4E-5E, MAB948). The human codon-optimized T7 polymerase plasmid was obtained from Addgene (#65974 ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were stained with different antibodies: mouse anti– rhodopsin 1D4 (1:1500, 1D4, Merck), rabbit anti-M-opsin (1:300 ...