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Citations for Merck :
1701 - 1750 of 2136 citations for 6 Chloro N ethyl 2 methylthio 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the cells at a density of 5×105 /ml were supplemented with 2% dimethyl sulfoxide (DMSO; Merck) and the culturing was continued for another 24 to 72 hr ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... dried tissue samples (in liquid nitrogen) were dissolved in 3:2:1 mixture of HNO3 (Merck, Germany), H2SO4 (Merck ...
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bioRxiv - Bioengineering 2022Quote: ... The nanobody was reduced using 10 mM Tris(2-carboxyethyl)phosphin -hydrochlorid (TCEP, #51805-45-9, Merck) on ice for 1h ...
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bioRxiv - Immunology 2022Quote: ... The loading pump delivered a solution of 0.1% formic acid in 98% water / 2% acetonitrile (Merck, Germany) at 10 µL/min ...
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bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Bioengineering 2022Quote: ... Five μL of the sample were injected into a C18 column (Merck Spherisorb ODS-2 (5 μm), 250×4 mm ...
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bioRxiv - Cell Biology 2023Quote: ... Lipids were dissolved in chloroform:methanol (2:1, v:v) and separated by TLC on silica gel 60 (Merck) along with a standard ...
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bioRxiv - Molecular Biology 2024Quote: ... coding sequences of AtCFI25a and AtCFI59 were cloned into pGEX5X-2 (GST) (Merck/GE Healthcare, Darmstadt, Germany) or pET30c (HIS ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 heads per sample were homogenised in 100 μL 2% SDS buffer (Cat No. 428018, Merck) containing 1X RIPA buffer (Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... in phosphate-buffered saline (PBS) and subsequently blocked in a solution containing 2% normal goat serum (Merck) within the same permeabilization solution ...
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bioRxiv - Immunology 2023Quote: ... were premixed at a 1:9 molar ratio or a 2:98 molar ratio in chloroform (Merck) to arrive at PLBs harboring Ni-DOGS-NTA at 10% or 2% ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM EE-analog in 150 µL of CH3OH were incubated with 2 mg/mL NaBH4 (Merck) in 150 µL of CH3OH at 37 °C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and 0.43 mM MgCl2) with the addition of 0.2 mM 1-phenyl-2-thiourea (PTU, Merck, Germany) to block pigmentation ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ml EDTA tubes were prepared with 100μl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany) before blood collection ...
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bioRxiv - Cancer Biology 2023Quote: ... formazan was solubilized by adding 2-propanol 0.1 M HCl (Sigma-Aldrich, Merck Life Science, Milan, Italy). The absorbance was measured at wavelength 570 nm (660 nm background wavelength ...
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bioRxiv - Plant Biology 2023Quote: ... pericarp sections were incubated for 2 h in a 5% (v/v) Normal Donkey serum (NDS; Merck) blocking solution in MTSB ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Cell Biology 2023Quote: ... and fertilized eggs were cultured in EmbryoMax KSOM Medium (1X) w/ 1/2 Amino Acids (Merck Millipore) at 37°C and 5% CO2 ...
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bioRxiv - Plant Biology 2024Quote: ... patens grown on PpNH4 were homogenized in 2 mL tubes using 3-mm zirconium glass beads (Merck) in presence of 500 µL of cold TEN buffer (Tris-HCl 100 mM ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was moved to 2 ml tubes and then 40% polysucrose 400 (P7798-100 g, Merck) in DPBS with calcium and magnesium was added in equal volumes for a final concentration of 20% ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... 100 μL of the faeces mixture was added to 10 mL of Selenite Broth (19 g/L selenite broth base, Merck, UK, 70153-500G and 4 g/L sodium hydrogen selenite, Merck 1.06340-50G) and incubated overnight at 37°C with shaking at 220 rpm ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Biochemistry 2022Quote: Collected samples from the first main peak were concentrated by ultrafiltration on 10K Amicon® Ultra-4 Centrifugal Filters Units (Merck Millipore) in a swinging-bucket rotor 4 000 g ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with Ringer’s solution followed by paraformaldehyde (PFA, 4 %, Merck Millipore, Darmstadt, Germany) in 125 mM phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2022Quote: ... Sonicated sample was diluted with 3.5 ml PBS and concentrated to 500 μl by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 KDa cutoff (Merck UFC803024) to remove traces of sarkosyl ...
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bioRxiv - Neuroscience 2022Quote: ... and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024) to remove the potentially unincorporated dye ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...