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Citations for Merck :
1701 - 1750 of 4431 citations for 6 Bromo 3 4 dihydro 2H 1 benzopyran 3 methanamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rats were anesthetized and underwent transcardial perfusion with 100 mL of saline followed by 100 mL of 4% paraformaldehyde (Merck, Germany) at 72 h following ICH induction ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Remaining chromatin extracts were incubated overnight at 4°C with 50 µL of prewashed anti-Flag M2 magnetic beads suspension (Millipore, Merck M8823) per sample ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Biophysics 2024Quote: ... The isolated protein was concentrated using Amicon® Ultra centrifugal filter with a molecular cutoff 10 kDa (Amicon Ultra-4 Centrifugal filter unit 10K, Merck). The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... 6xHis:SidK:3xFLAG or 6xHis:SidKF62A:3xFLAG protein were added to 1 ml of filtered extract to a final concentration of ∼2.5 μM and incubated in a rotatory mixer at 4°C with 25 μl of Anti-FLAG® M2 Magnetic Beads (Merck). 30 μl of the mixture was taken as input for Western blot analysis before adding magnetic beads ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized in 1% DMSO/1% Triton X-100 and stained with anti-phospho H3 antibody (1:250, Merck Millipore) followed by anti-rabbit Alexa 488 secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL lysis buffer (15 mM NaHEPES pH 7.3, 30 mM NaCl, 1 mM MgCl2, 1% Brij35, 1 U/µL benzonase (Merck), 1X complete protease inhibitor (EDTA-free ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
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bioRxiv - Microbiology 2021Quote: ... The suspension was treated with DNase I (1 μg ml-1) and RNase A (100 μg ml-1, Merck, India) to degrade nucleic acid residues of the host bacteria ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in 8 mL lysis buffer (5 mM imidazole in 100 mM potassium phosphate buffer pH 8.0) supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-PSD-95 (Merck Millipore, cat 04-1066, 1:200; 1:1,000); anti-vGlut (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Penstrep and 1 ng/ml TGFβ (Cat nb. T7039, Merck, Sigma) or 20 ng/ml PDGF-AA (human PDGF-AA ...
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bioRxiv - Biophysics 2022Quote: ... cells were diluted in in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine to 50,000 cells/ml and plated at a density of 5000 cells per well (100 µl ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 1 mM PMSF and 1 X cOmplete protease inhibitors (Merck) and lysed with a French press under 1200 PSI ...
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bioRxiv - Immunology 2019Quote: ... The sections were fixed in a 1:1 mixture of methanol:acetone (Merck) and blocked with PBS containing 5% goat serum and 5% mouse serum (blocking solution) ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... and goat anti-Mouse IgG-HRP (1:1000-1:3000, Merck Millipore). The kinase reactions using recombinant McIdas were performed as follows ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... and goat anti-Mouse IgG-HRP (1:1000-1:3000, Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL (1:500 dilution) of biotinylated anti-phosphotyrosine 4G10 (Merck) antibody and incubated for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).
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bioRxiv - Biochemistry 2024Quote: ... 1:1’000) and mouse anti-EF1a (Merck Millipore, dilution WB 1:10’000). Antibodies previously produced in our laboratory are ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 mM MgCl2 1 mM DTT and 1× cOmplete protease inhibitor (Merck)] were added and incubated on the rotating wheel for 2 h at 4ºC ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:1 mixture of magnetic hydrophobic and hydrophilic Seramag speedbeads (MERCK) were added at a 2:1 (w/w ...
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bioRxiv - Genetics 2024Quote: Cells were lysed in 1% SDS plus 1:200 Benzonase (Merck, #E1014) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Microbiology 2019Quote: ... 100 μL of the faeces mixture was added to 10 mL of Selenite Broth (19 g/L selenite broth base, Merck, UK, 70153-500G and 4 g/L sodium hydrogen selenite, Merck 1.06340-50G) and incubated overnight at 37°C with shaking at 220 rpm ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
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bioRxiv - Biophysics 2020Quote: Upon deposition the samples were washed 2 times 15 minutes with PBS prior to overnight fixation with 4% paraformaldehyde (PFA, Merck, Darmstadt, Germany). Washing steps and incubation took place under agitation at 4 °C unless stated otherwise ...
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bioRxiv - Biochemistry 2022Quote: Collected samples from the first main peak were concentrated by ultrafiltration on 10K Amicon® Ultra-4 Centrifugal Filters Units (Merck Millipore) in a swinging-bucket rotor 4 000 g ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with Ringer’s solution followed by paraformaldehyde (PFA, 4 %, Merck Millipore, Darmstadt, Germany) in 125 mM phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2022Quote: ... Sonicated sample was diluted with 3.5 ml PBS and concentrated to 500 μl by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 KDa cutoff (Merck UFC803024) to remove traces of sarkosyl ...
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bioRxiv - Neuroscience 2022Quote: ... and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024) to remove the potentially unincorporated dye ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
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bioRxiv - Microbiology 2024Quote: ... the same dilutions were also asdded to Eosin Methylene Blue Agar and MacConkey Agar both supplemented with cefotaxime (4 mg/L) (Merck, Darmstadt, Germany) for the Extended-spectrum beta-lactamase phenotype (37) ...
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bioRxiv - Plant Biology 2024Quote: The following chemicals were used in the DEEP-Clear (Pende et al., 2020) adapted version to plant tissues: PFA (paraformaldehyde) (CAS no. 30525-89-4, Merck, http://www.merck.com/), xylitol (CAS no ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...