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Citations for Merck :
1651 - 1700 of 2756 citations for 6 methyl 3 oxo 2 3 dihydropyridazine 4 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2 ml CaCl2 (2.5 M; Merck; Catalog Cat.nr. 1023820500) and 12 ml of ddH2O were slowly added (5 ml/15 cm plate ...
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bioRxiv - Genetics 2024Quote: ... fixed for 20 min 2% paraformaldehyde (Merck Life Science) and for 30 min in 75% ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM Glutamax (gibco) and 10 mM nicotinamide (Merck). After 24 days ...
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bioRxiv - Immunology 2024Quote: ... Each construct was transformed into BL21 Rosetta 2 (Merck) and grown in LB at 30 °C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: RNA for each biological repeat was extracted from 110 mg of rosette leaves number 5-6 (from at least six plants) with TRI Reagent (Merck) and rounds of phenol-chloroform and chloroform extractions followed by isopropanol precipitation ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 million cells were plated per well in a 6-well plate and cultured for 48h in the presence or absence of 200 nM smoothened agonist (Merck). RNA was extracted and cDNA was generated with Superscript II kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... were assessed by analytical gel filtration using an Akta FPLC UPC-900 equipped with a Superose 6 Increase 10/300 GL column (Merck). Typically ...
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Microbiology 2021Quote: ... NHS-ester staining: the gel was placed in a 6 well plate and incubated in 594 NHS-ester (Merck, 08741) diluted at 10 μg/mL in 3 ml PBS for 1 hour and 30 minutes at room temperature on a rocking platform ...
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bioRxiv - Immunology 2020Quote: ... The thorax and the skin incision were closed with 6/0 silk sutures (Lorca Marín) and buprenorphine (0.01 mg/kg, Buprex, Merck & Co. Inc) was given for pain relief ...
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bioRxiv - Immunology 2023Quote: ... WT mice were 7-8 week-old Mice (C57BL/6 WT or Nr4a3-Timer-GS) were injected with 10 mg/kg azoxymethane (Merck) via i.p ...
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bioRxiv - Microbiology 2023Quote: R47 and R32 wild type strains were inoculated and grown in 6-well plates as described in the RNA extraction section except that 1 ml 0.38 M NaOH (Merck, Germany) was added to the middle two wells to capture HCN in the surrounding ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants (35 mL) were removed at day 6 and day 10 and filtered through a Miracloth (Merck Milipore, Carrigtwohill, Ireland) into a clean tube ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Neuroscience 2024Quote: ... the myelinated slices were incubated 16 hours at 6 DIV in 0.5 mg/ml L-α-Lysophosphatidylcholine (LPC, 830071P, Avanti, Merck) added to fresh culture medium.
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bioRxiv - Genomics 2019Quote: ... heads of 10 ISO-1 females were chopped in 500 µL of ice-cold Otto 1 Buffer (0.1 mol/L citric acid, 0.5% Tween20 (Merck KGaA, Darmstadt, Germany)) ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Molecular Biology 2019Quote: To evaluate microscopic features of the captured cells such as morphological types and possible alterations we then used another collection membrane directly stained with hematoxylin and eosin after fixation for 10 min in a fixation solution (100 mL of 70% ethanol, 5 mL of glacial acetic acid and 5 mL of 37% formaldehyde solution – all solutions are from Merck Millipore). Samples were hydrated with distilled water for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs of mouse BRME1 (amino acid residues: 540-578 and 540-574) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6 tag ...
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bioRxiv - Systems Biology 2024Quote: ... The solvent system comprised solvent A consisting of 0.1% formic acid in Milli-Q water (both HPLC grade, Merck, Darmstadt, Germany) and solvent B being methanol (UHPLC grade ...
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bioRxiv - Microbiology 2023Quote: ... Samples were passed through a 0.2 µm nylon membrane prior to analysis with liquid chromatography as described previously 50. Separation for organic acid quantification (i.e. SCFAs and intermediate metabolites) was conducted on a LaChrom HPLC-System (Merck-Hitachi, Japan) using a SecurityGuard Cartridges Carbo-H (4 × 3.0 mm ...
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... Heads including both eyes were fixed in 4% w/v paraformaldehyde (PFA, Merck) in 0.1M phosphate buffer overnight at 4°C and the remaining tissue were kept for genotyping by conventional PCR ...
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bioRxiv - Microbiology 2021Quote: ... concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck), and stored at -80°C in a storage buffer [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... Tissues were embedded in 4% low gelling temperature agarose (Merck, Cat#A 0169) and sectioned by vibratome (Leica VT1200 S ...
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bioRxiv - Neuroscience 2020Quote: ... and mounted on glass slides with Mowiol 4-88 (Merck Millipore, Burlington, USA). Confocal images were obtained with a confocal laser scanning microscope (LSM780 ...
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bioRxiv - Molecular Biology 2022Quote: ... The homogenized solutions were filtered (Amicon Ultra-4, 50k, Merck KGaA, Darmstadt, Germany) to remove particle residuals and proteins from clogging the HPLC column ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed in PBS and fixed with 4% paraformaldehyde (Merck KGaA) in PBS for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sections were stained with 4 % uranyl acetate (Serva) and Reynold’s lead citrate (Merck). Micrographs were taken with a Zeiss EM 906 transmission electron microscope at 80 kV acceleration (Carl Zeiss ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 4% PFA diluted in PBS (37% Formaldehyde solution, Merck) and stained with 2% crystal violet solution (HT90132-1 L ...
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bioRxiv - Neuroscience 2020Quote: ... blocked again and incubated overnight at 4°C with DAPI (1:2000) (Merck) and anti-rabbit AlexaFluor-488 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was stained with 4 ug/ml Hoechst 33342 (Merck KGaA Cat# B2261).
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bioRxiv - Molecular Biology 2019Quote: ... 40 times by centrifugal filtration (3K Amicon Ultra, Merck Millipore, 14’000g, 4°C) and buffer was exchanged to 4M Guanidine hydrochloride in 50mM HEPES (pH 7.8) ...
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bioRxiv - Microbiology 2020Quote: ... concentrated at 4°C using a 10 kDa spin concentrator (Merck Millipore, USA) and centrifuged for 1 h at 15,000 g at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were pre-bound with 4-5 micrograms of antibodies (H3K4me3 – Merck 07-473 ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments with Poly(sodium) 4-styrenesulfate (PssNa) (243051; Sigma-Aldrich/Merck group, USA), a polyelectrolyte able to scavenge charged molecule 39 ...
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bioRxiv - Cell Biology 2022Quote: ... Bacteria were then lysed by adding BugBuster (Merck Millipore, cat. no. 70584-4) and a small spoonful of lysozyme (Thermo Scientific ...
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bioRxiv - Genetics 2022Quote: Fat bodies were fixed in 4% Formaldehyde (Science Services) and 2,5% glutaraldehyde (Merck) in 0.1M Cacodylate buffer (AppliChem ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with DAPI and mounted in Mowiol 4-88 (Merck Chemicals). For the detection of Aβ ...
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bioRxiv - Developmental Biology 2022Quote: Samples were washed twice with PBS and fixed using 4% formaldehyde (1.04005.1000, Merck) in 0.1 M Na+/K+-phosphate buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... adjacent samples were fixed for fifteen min in 4% PFA (Merck, Darmstadt, Germany) diluted in PBS (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... pH ∼6.9) immediately followed by circulation of 4% freshly depolymerised paraformaldehyde (PFA; Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Biophysics 2023Quote: ... concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024), and applied to a Superdex 200 increase 10/300 GL column (GE Healthcare #28-9909-44 ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 was purified with Protein G Sepharose 4 Fast Flow beads (Merck), washed with PBS ...