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Citations for Merck :
1601 - 1650 of 1938 citations for Methyl 4 2 methoxynicotinoyl benzoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Microbiology 2023Quote: ... Residues in solution were pelleted by centrifugation at 12000Xg for 30 minutes at 4℃ and soluble fraction was filtered through 0.22-micron filters (Merck Millipore Ltd, Cork, IRL). Supernatant was loaded to a 5 ml Nickel-Sepharose HisTrap Chelating column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were placed in a humid chamber and cells were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse monoclonal anti-FLAG clone M2 (Merck F1804; 1:1000), rabbit monoclonal anti-KTN1 clone D5F7J (Cell Signaling Technology #13243 ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2.5 × 105 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 µg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Bioengineering 2020Quote: ... except that 2-monoacylglycerols were separated by thin layer chromatography (Silica gel 60, 20 × 20 cm; Sigma-Aldrich/Merck) using hexane:diethylether:acetic acid (35:70:1.5 ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μL of each sample was injected into a Chromolith FastGradient RP 18-e column (50 x 3 x 2 mm; monolithic silica with bimodal pore structure, macropores with 1.6mm diameter; Merck) protected by a precolumn (Gemini NX RP18 ...
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bioRxiv - Immunology 2021Quote: ... Biochemical studies of blood serum samples were carried out on VITALAB SELECTRA 2 in an automatic analyzer (Merck, Germany) using commercial kits (DIASYS Diagnostic Systems GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... The floated sections were rinsed briefly with 100 mM maleate buffer (pH 7.5) and blocked for 30 minutes against non-specific antibody binding with 2% blocking reagent (Merck) in maleate buffer ...
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bioRxiv - Biophysics 2022Quote: ... the sample was placed on ice and the proteinase activity was quenched with 2 mM phenylmethylsulfonyl fluoride (Merck, P7626) in DMSO.
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bioRxiv - Immunology 2022Quote: NPC cellular phenotypes were induced in RAW 264.7 and J774.A MΦ by treating with 2 μg/ml U18666A (Merck) for 30h or 72h respectively ...
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bioRxiv - Cell Biology 2022Quote: ... the nuclei were visualized with Hoechst 33258 (Merck, B2883, 2 µg/ml in H2O at RT for 5 min).
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bioRxiv - Cell Biology 2022Quote: ... resolubilized in 25 µL of 0.05% trifluoroacetic acid in 98:2 H2O/acetonitrile (v/v) and filtrated through a 0.2 µm spin filter (Merck Millipore Ultrafree®-MC ...
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Microbiology 2020Quote: The TLC was performed by spotting 2 μL of enzymatic reaction on a silica gel 60 F454 plate (Merck), the separation was carried out in butanol ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v) ACN (Merck, USA) containing 0.1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... the obtained 2-cell stage embryos were transferred to a fresh drop of KSOM (Merck, cat. # MR-107-D). The IVF with b6d2F1 oocytes and b6casF1 has on average a 2-cell development (fertilization ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck, https://www.merckmillipore.com] containing 50 mM N-ethylmaleimide (NEM ...
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bioRxiv - Plant Biology 2023Quote: ... dried and transferred in a grid pattern onto square plates containing 50 ml of MS/2 medium (0.5× Murashige and Skoog salts [Merck Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Molecular Biology 2022Quote: ... The Lipid II extract was assessed by spotting 1-2 μL on a HPTLC silica gel 60F254 plate (Merck). The TLC plate was developed in a mixture of chloroform:methanol:water:ammonia (88:48:10:1 ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Plant Biology 2022Quote: ... the CDS of wild type (WT) PpGS1b.1 and PpGS1b.2 were subcloned into pET30a vector (Merck, Darmstadt, Germany) including a N-terminal 6xHis-tag by PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were cleaned with a solid phase extraction method using 2 layers of C18 Empore™ SPE Disks (Merck) followed by 2 layers of SCX strong cation exchange Empore™ SPE Disks (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in xylene for at least 2 min and then covered with mounting solution Entellan ® (Merck). Images were acquired at a S360 Hamamatsu slide scanner using a 40x objective ...
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bioRxiv - Neuroscience 2024Quote: ... concentrated using 100-kDa molecular weight cut-off (MWCO) filters Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at - 80°C ...
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bioRxiv - Plant Biology 2024Quote: A pTYB2-based vector for the expression of PSM polypeptides and a pCDF-based vector that contained heme oxygenase 1 and phytochromobilin synthase genes for the synthesis of PΦB (Muramoto et al., 1999; Kohchi et al., 2001) were transformed into the Escherichia coli Rosetta 2 strain (Merck). E ...
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bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
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bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected as control and the beads were thoroughly washed 3 x with TBS/Tween 0.2% after which bound proteins were eluted by adding hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck) and boiling for 5 min at 99 °C ...
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bioRxiv - Cell Biology 2024Quote: ... medium—composed of 6.7 g Yeast Nitrogen Base without amino acids (Difco 291940) and 0.8 g complete supplement mixture drop-out (Formedium DCS0019) in 1 L—supplemented with 2% glucose (Merck). Cells from glycerol stocks or agar plates were inoculated into YPD and incubated at 30°C on an orbital shaker at 250 rpm (in an Innova 4230 incubator ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...
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bioRxiv - Biophysics 2023Quote: ... in digestion buffer (800 mM guanidine HCl, 2 mM CaCl2, 0.5 % Triton X-100, in 50 mM TRIS, #8382J008706, Merck), overnight at 50°C ...
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bioRxiv - Cell Biology 2023Quote: ... To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml, Merck, Cat.N. 3072) for the duration of the experiment ...
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bioRxiv - Immunology 2023Quote: ... Afterward, the cells were washed twice in R10 media (RPMI-1640 with 10% FBS, 2 mM glutamine (G7513, Merck), 1% penicillin-streptomycin (P0781 ...
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bioRxiv - Immunology 2023Quote: ... the mice were intranasally sensitized using 1 μg of 2′3′-cyclic GMP-AMP (cGAMP, 531889; Merck Darmstadt, Germany) and 1 μg of (HDM ...
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bioRxiv - Genomics 2023Quote: ... a stock solution of 2 mg/mL colistin sulfate (Sigma-Aldrich Merck KGaA, Germany; 100 mg, 15,000 U/mg) was prepared and aliquots were stored at −80 °C ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed trice for 10 min with 0.05% Tergitol in TBS (Tris-Buffered Saline) and blocked for 1 hour with 2% BSA (Merck) in TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... They were further incubated with a 2× volume of 0.25× diluted TES buffer with a trace amount of benzonase nuclease (Merck) at 4°C for 45 min.