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Citations for Merck :
1551 - 1600 of 2632 citations for Mouse Anti Hepatitis C Virus E2 Antibody 1876 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... protoplasts were overlaid with ½-strength PDA cooled-down to ~50°C and supplemented with 50 mg/ml hygromycin B (Merck). Transformants appearing on the PDA surface ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Microbiology 2021Quote: ... The entire 20 l volume was then filtered through a GF/C filter (Whatman, Maidstone, United Kingdom) and 0.45 µm PVDF filter (Durapore, Merck Millipore) to eliminate cells and cellular debris.
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bioRxiv - Microbiology 2022Quote: ... “treated” cultures were grown with the addition of 0.25 µg ml-1 mitomycin C (MMC) (Merck Life Sciences UK Ltd). Mycelial pellets from untreated and treated cultures were washed in 1x PBS before lysis and RNA purification using the RNEasy Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Cecum content was collected from freshly sacrificed mice and stored at -70 °C in anaerobic Brain Heart Infusion (BHI) broth (Merck) containing 20% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... Nuclei were pelleted by centrifugation at 3000 rpm for 5 min at 4°C and were resuspended in hypotonic buffer containing 1U/µl of benzonase (Merck). Extracts were incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... and CM was concentrated to 1 ml at 4 °C using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
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bioRxiv - Biochemistry 2019Quote: ... A single colony of freshly transformed cells was cultured at 37 °C in 500 mL 2xYT medium supplemented with 50 µg/mL kanamycin (Merck) and 25 µg/mL chloramphenicol (Sigma Aldrich) ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... and the plates were incubated at 33 °C in a 5% CO2 atmosphere in fresh medium with 0.25% Hoechst (Merck-Millipore) to stain the DNA ...
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bioRxiv - Immunology 2021Quote: Culturing medium was changed 24 h post-transfection and vector supernatants were collected 48 h after transfection and processed by centrifugation at 1000 g for 10 mins at 4°C to remove cellular debris followed by microfiltration using Millex-HV 0.45 μm syringe filter units (Merck – SLHV033RB). Viral supernatants were either kept on ice for further use or frozen down at −80°C for storage.
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bioRxiv - Molecular Biology 2022Quote: Conditioned medium was centrifuged at 3,200xg for 10 min at 4°C to remove debris and transferred to 10 kDa spin columns (Amicon Ultra, Merck). Proteins that may influence lactate and pyruvate level were removed from the <10 kDa flow-through containing metabolites by centrifugation at 14,000xg for 25 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
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bioRxiv - Molecular Biology 2022Quote: Cells were centrifuged at 3041 × g for 30 minutes at 4°C and supernatant was applied to a 500 mL Stericup-HV sterile vacuum filtration system (Merck) with a pore size of 0.22 µm ...
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bioRxiv - Microbiology 2023Quote: ... Clarification was then performed by centrifugation for 1 hour at 12,000g and 4°C and vacuum filtration using 45um nylon filter systems (SteriFlip - Merck Millipore). Prior to purification ...
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bioRxiv - Biochemistry 2022Quote: ... as described by the manufacturer and dried down in a Speed-Vac concentrator (Thermo-Scientific) resuspended in 20 μl L/C water containing 3% acetonitrile (MeCN) (Merck) and 0.1% FA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 2 ml 4 °C PBS for 3 times and afterwards fixed in 1 ml of −20 °C cold methanol (Gradient grade for liquid chromatography, Merck) for 5 minutes at −20 °C ...
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bioRxiv - Microbiology 2022Quote: ... through six dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with 10 kDa molecular weight cutoff (Merck). For experiments studying the IgG-binding properties of Fc-III and Scr ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS for ten minutes at 4 °C and then washed in 0.1% Tween20 in PBS (PBST) (Merck, Darmstadt, Germany) and blocked with 5% goat sera (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... digestion of roots was performed at 90°C during 4 h in 400 µL of 65%(w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.65% (w/v ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated for 10 minutes at 37°C with Liberase (250 μg/ml) and DNAse (50μg/ml Merck®). After incubation ...
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bioRxiv - Neuroscience 2024Quote: Tissues were dissected out and homogenized at 4°C in RIPA buffer containing protease inhibitors cocktail (Merck Millipore, Milano, Italy) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... We stored them at -80 °C in phosphate buffered saline (PBS) with 20 % (v/v) glycerol (anhydrous; Merck, Darmstadt, Germany) and cultivated the isolates on Columbia blood agar plates containing 5 % sheep blood (Mast Diagnostica ...
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... The next day the cleaved protein was collected and concentrated by spinning the sample at 3500 RPM at 4°C using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and boiled for 5 min at 99°C in hot reducing SDS-buffer containing 2-mercaptoethanol (Merck).
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bioRxiv - Cell Biology 2023Quote: ... Epiphyses were embedded in low melting agarose and tibias were placed for 30 min at 37°C in digestion medium composed of PBS with 3mg/ml of Collagenase B (C6885, Merck), 4mg/ml of Dispase (D4693 ...
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bioRxiv - Biophysics 2024Quote: ... Germany) for 4 h at 4°C followed by a washing in 0.1 M Soerensen’s Phosphate Buffer (Merck, Darmstadt, Germany). The dehydrating step was performed in the ascending alcohol series with 10 minutes duration each ...
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bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in T cell medium for 30 min at 37°C without any metabolic inhibitors or in presence of 1 μM oligomycin (Merck), 100 mM 2DG (Merck) ...
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bioRxiv - Immunology 2023Quote: ... isolated cells from WAT were counted using cell counting chambers (one-way Neubauer counting chambers, C-Chip, Merck, Darmstadt, Germany). Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit ...
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bioRxiv - Neuroscience 2023Quote: ... heated to 37°C with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin79 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Immunology 2023Quote: ... for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest) and 10% DMSO (Merck) using a cell freezing container (CoolCell LX ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 40 µL was incubated (1h at 70°C on a heating block) by addition of 20 µL of the trimethylsilylating (TMSi) reagent (chlortrimethylsilane [Merck KGaA ...
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bioRxiv - Microbiology 2023Quote: ... JKD6004 and the JKD6009/JKD6008 (VSSA/VISA) pair were routinely cultured at 37°C on solid or in liquid brain heart infusion (BHI, Merck) or Mueller-Hinton (MH ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were harvested after at least 24 h of incubation at 32°C and filtered on a 0.45 µm filter (Merck #SE2M230I04).
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bioRxiv - Biophysics 2024Quote: ... HeLa cells were cultivated at 37 °C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 2.5 µg mL-1 α-factor grown at 25°C (Cat. No. T6901, Merck). After 90 min ...
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bioRxiv - Biophysics 2024Quote: ... stored at −80 °C and diluted to the final concentration in the intracellular recording solution.Halothane 99 % (Sigma-Aldrich/Merck, Germany), isoflurane (Baxter ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation (20.000xg, 15 min, 4°C) and protein concentration was determined by Direct Detect® spectrometer (Merck). The proteasome activity was measured using a 96-well plate ...
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bioRxiv - Microbiology 2024Quote: ... foam formation was reduced with a few drops of six times diluted antifoam C 391 emulsion (Merck Life Science NV). For supernatant analysis ...