Labshake search
Citations for Merck :
1501 - 1550 of 1792 citations for Rat Insulin Like Growth Factor Binding Protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were combined and a buffer exchange and the protein were concentrated with a 30 K Amicon® Ultra Centrifugal Filter spin column (Merck KGaA) to the storage buffer (20 mM HEPES pH 7.4 ...
-
bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
-
bioRxiv - Cell Biology 2022Quote: ... Separated proteins were then transferred by wet tank-blotting (100 V for 60 min) onto a polyvinylidene difluoride (PVDF) membrane (Merck, Darmstadt, Germany). The membrane was blocked with Roti®-Block (2 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
-
bioRxiv - Immunology 2022Quote: ... at pH 2.0 with final protein concentration of 1.75 mg/mL (Sphβ preparation) or in 40% ethanol (v/v from ethanol absolute, Merck KGaA, Darmstadt, Germany), 0.25 M NaCl at pH 1.8 with final protein concentration of 5 mg/mL (Sphα preparation) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Total protein from tissue extracts or plasma were prepared in Laemmli or RIPA buffers (both from Merck Life Science UK Ltd, UK), separated by polyacrylamide gel electrophoresis and then transferred to either nitrocellulose or a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
-
bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
-
bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... diluting 3 μl of protein sample (20 nM) in 15 μl PBS buffer loaded on a gasket (Grace Bio-Labs reusable CultureWell gaskets (GBL103250, Merck)) mounted on a glass microscope coverslip (0107222 ...
-
bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
-
bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
-
bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
-
bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Polyvinylidine difluoride membrane and ECL kit (WBKLS0500) were purchased from Merck Millipore (Billerica ...
-
bioRxiv - Genetics 2020Quote: ... Chromatin immunoprecipitation was performed using Agarose ChIP Assay Kit (Merck Millipore). Briefly,107 cells were lysed by incubation on ice with 130 µl lysis buffer for 15 minutes ...
-
bioRxiv - Cancer Biology 2020Quote: Chromatin immunoprecipitation assay was performed using a commercial kit (Merck-Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunoreactive bands were detected using ECL detection reagent kit (Merck-Millipore) and the FusionSL chemiluminescence documentation system (Vilber-Lourmat) ...
-
bioRxiv - Cell Biology 2022Quote: ... H&E staining was carried out using a kit (Merck, Germany). Muscle fiber cross sectional area was measured with the ImageJ 1.48 software (National Institutes of Health ...
-
bioRxiv - Cell Biology 2022Quote: Following cytospin of leukocytes the Hemacolor® staining kit (Merck, Germany) that works based on eosin (red)/azur (blue ...
-
bioRxiv - Neuroscience 2021Quote: ... and antibodies were diluted with SignalBoost™Immunoreaction Enhancer Kit (Merck). Primary antibodies included phospho-TDP-43 (Ser409/410 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Collagen was detected with the Van Gieson kit (Merck, Darmstadt, Germany), and reticulin ...
-
bioRxiv - Biochemistry 2020Quote: The PKH26 Fluorescent Cell Linker Mini Kit was purchased from Merck-Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... and stained using the AldeRed ALDH detection assay kit (Merck SRC150) according to manufacturer specifications.
-
bioRxiv - Genomics 2022Quote: ... Keratanase (G6920-5UN)) and a glycoprotein deglycosylation kit (Merck catalog #362280). We then used Water-18O (97% atom ...
-
bioRxiv - Neuroscience 2023Quote: ... Congo Red staining was performed using Congo Red Staining Kit (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: BACE-1 activity was assessed using a fluorescent assay kit (Merck), while plasma glycerol and plasma HDL-C were determined using colorimetric kits (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: ... and the bound IgG was eluted using Ab Buffer Kit (Merck), and then the eluate was dialyzed overnight at 4°C in PBS using a 10 kDa dialysis membrane (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
-
bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2022Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (Merck S7165) was used ...
-
bioRxiv - Cell Biology 2022Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
-
bioRxiv - Genomics 2023Quote: ... The Sigma Spectrum plant total RNA kit (Merck KGaA, Darmstadt, Germany) was used for RNA extraction that followed the manufacturer procedure ...
-
bioRxiv - Genetics 2023Quote: ... using the Spectrum Plant Total RNA kit (Merck KGaA, Darmstadt, Germany). Gene expression was determined by RT-qPCR as previously described (Zenoni et al. ...
-
bioRxiv - Physiology 2023Quote: ... glucose levels were determined using a Glucose (GO) assay kit (Merck). Blood insulin concentrations were measured by Rat Insulin ELISA kit (Mercodia) ...
-
bioRxiv - Cell Biology 2022Quote: ... Aliquots were retained for direct Western blot analysis (Input) and otherwise subjected to immunoprecipitation (IP) with protein A-coupled Sepharose beads (Merck, 17-5280-02) pre-incubated with 2 μg anti-RbBP5 antibody or IgG at 4°C for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... Measurements were run in triplicates diluting the protein sample at 50 nM concentration in NMR buffer loaded on a gasket (Grace Bio-Labs reusable CultureWellTM gaskets, Merck (#GBL103250)) mounted on a glass microscope coverslip (Marienfeld (#0107222)) ...
-
bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
-
bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
-
bioRxiv - Neuroscience 2022Quote: Equal amounts of total protein (50 μg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal amounts of total protein (50 µg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
-
bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
-
bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
-
bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...