Labshake search
Citations for Merck :
1501 - 1550 of 1830 citations for 6 Amino 2 4 difluorobenzotrifluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... the cells were washed three times in 0.1 M sodium cacodylate buffer pH 7.2 ± 0.1 containing 0.2 M sucrose and 2 mM MgCl2 (Merck Millipore), and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co ...
-
bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore ...
-
bioRxiv - Biophysics 2023Quote: ... exposure was evaluated by a MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test in a 96-well plate according to the manufacturer’s (Merck) protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... the mice were inoculated with 100,000 SB28-GFP cells suspended in 2 µl of DMEM (Merck, Darmstadt, Germany) (glioblastoma mice ...
-
bioRxiv - Molecular Biology 2023Quote: Cells of 2-3 days growth with approximately 70-90% confluency have been treated with Bortezomib (Merck, #504314) at 100 nM for 42 h ...
-
bioRxiv - Microbiology 2023Quote: ... auris cells (CHRIST Alpha 1-2 LD plus lyophilizer, Osterode, Germany) using Tri Reagent (Merck Ltd. Budapest, Hungary). The quality of RNA was determined using the Eukaryotic Total RNA Nano kit (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
-
bioRxiv - Microbiology 2023Quote: ... A fraction of the inoculum was directly transferred into 2 ml tube containing TRI reagent (Sigma-Aldrich, Merck) and silica beads (0.1 mm diameter ...
-
bioRxiv - Biophysics 2023Quote: ... 5 and 10 μl of the lipid extract 2 cm from the bottom on aluminum-backed silica gel 60 TLC plates (cat#: 1.05553.0001; Merck) together with DOPG ...
-
bioRxiv - Molecular Biology 2022Quote: ... eggs rafts were hatched in trays containing 2 L of Milli-Q water (Synergy® UV, Merck, Germany). Larvae were fed continuously until the pupae stage with TetraMin® baby fish food (Tetra ...
-
bioRxiv - Molecular Biology 2023Quote: ... exponentially growing RPE-1 cells were pulse-labeled with 1 μM CldU (5-Chloro-2’-deoxyuridine, Merck, C6891) and 250 μM IdU (5-Iodo-2’-deoxyuridine ...
-
bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped with 2 µl of 0.5 M EDTA and separated using thin layer chromatography plates (Merck) with 0.3 M LiCl and 0.3 M formic acid as the mobile phase ...
-
bioRxiv - Biochemistry 2023Quote: The reaction mixtures (0.5, 1 or 2 µL) were spotted onto TLC Silica Gel 60 F254 plates (Merck). As for analysis of cyclization activity ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human HepaRG hepatoma cells (1.5-2×105 cells/cm2, Biopredic International, Rennes, France) were cultured in William’s E medium (Merck) supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Zoology 2024Quote: ... The sample was then placed in 2 ml petroleum ether (boiling range 40-60°C, Merck, Darmstadt, Germany) at room temperature for two days ...
-
bioRxiv - Molecular Biology 2024Quote: ... The bound complexes were eluted in lysis buffer 2 containing 25 mM glutathione or 5 mM biotin (Merck), respectively ...
-
bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... L-glutamine and antibiotic/antimycotics and decidualized with 1µM PGE2 or 1µM relaxin-2 (accession # P04090) (both Biotechne Ltd, Abbington, U.K.) in combination with 1µM medroxyprogesterone acetate (MPA) (Merck) for up to 4 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... A total of 2 × 104 cells were cultured in an 8-well chamber slide (Merck Millipore, Massachusetts, USA) and then fixed with 4% paraformaldehyde in PBS buffer for 15 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
-
bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Plant Biology 2020Quote: About 8,000 T4 seeds from the W29-1-4 reporter line were mutagenized by treatment with 0.3% (v/v) ethylmethanesulfonate (Merck KGaA, Frankfurter, Darmstadt, Germany) for 15 h at 25°C ...
-
bioRxiv - Physiology 2021Quote: ... All incubations with primary antibodies were performed for 16 hours at 4°C. Blots were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
-
bioRxiv - Plant Biology 2020Quote: ... Conidia for either seeding liquid cultures or for use in pathogenicity assessments were collected by washing 4-week-old MM cultures with sterile water (SW, Milli-Q Integral Water Purification System for Ultrapure water, Merck KGaA, MA, USA), then filtered through glass wool to remove mycelial debris ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells and supernatants were harvested and purified using chloroform and were condensed using Amicon Ultra-4 Centrifugal Filter Devices (100K MWCO; Merck Millipore, Billerica, MA).
-
bioRxiv - Cell Biology 2022Quote: ... centrifuged at 3,000 × g at 4°C and filtered under sterile conditions using a 0.22 μm filter (Millex-GV, PVDF; Merck/Millipore Watford, Hertfordshire, UK), and is referred to as CM-OAT1.
-
bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
-
bioRxiv - Plant Biology 2022Quote: ... followed by a 20 min centrifugation at 4°C and 4000 rpm using 10-kD Amicon®Ultra centrifuge filter (Merck, Darmstadt, Germany) to remove free (proteinun bound ...
-
bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck, Buchs Switzerland). Memofl/fl littermates without Cre but treated with tamoxifen served as controls ...
-
bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
-
bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
-
DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
-
bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
-
bioRxiv - Microbiology 2022Quote: Non-infected and MCMV-infected mice were orally gavaged with 150 μl of 100 mg/ml 4 kDa FITC-dextran-labeled dextran beads (Sigma-Aldrich/Merck, Darmstadt, Germany) in PBS 4 hours prior to sacrifice ...
-
bioRxiv - Molecular Biology 2023Quote: ... lysates were clarified by centrifugation at 12000g and incubated 2h at 4°C with pre-equilibrated α-FLAG M2 affinity gel beads (Merck-Sigma, A2220). Beads were washed three times with lysis buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
-
bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
-
bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
-
bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
-
bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
-
bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
-
bioRxiv - Microbiology 2023Quote: ... Residues in solution were pelleted by centrifugation at 12000Xg for 30 minutes at 4℃ and soluble fraction was filtered through 0.22-micron filters (Merck Millipore Ltd, Cork, IRL). Supernatant was loaded to a 5 ml Nickel-Sepharose HisTrap Chelating column (GE Healthcare ...
-
bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
-
bioRxiv - Biophysics 2019Quote: ... Fractions containing Rab8-His6 were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and injected into a Superdex 75 16/60 (GE Healthcare ...
-
bioRxiv - Cell Biology 2021Quote: ... 1-2.5 × 105 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 µg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM ...
-
bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...